A Microfluidic Detection Chip Based on Droplet Experiment
A detection chip and microfluidic technology, applied in the measurement/inspection of microorganisms, enzymology/microbiology devices, bioreactors/fermenters for specific purposes, etc., can solve problems such as unfavorable fluorescent signal detection, and achieve improved detection The effect of precision
Active Publication Date: 2017-07-25
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Problems solved by technology
[0007] Even though the droplet technology has many advantages, it still faces some technical difficulties in the process of high-throughput detection. Adjustment
High packing density is conducive to batch thermal cycling of droplets, but it is not conducive to the detection of fluorescence signals of independent droplets. Therefore, it is necessary to design a detection chip to separate independent droplets after the droplet PCR reaction. , to improve detection accuracy
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[0019] Below in conjunction with accompanying drawing and embodiment the present invention will be further described:
[0020] like figure 1 As shown, an optical detection system includes a droplet channel network, a stray light detector 6 and a fluorescence detector 7 . An optical device is placed between the micro-droplet 5 to be detected and the stray light detector 6 to play the role of concentrating light.
[0021] When the radiation source 8 encounters the droplet 5, it will partially scatter. The stray light detector 6 is used to detect the properties of the droplet 5, such as the surface morphology of the droplet 5, etc. This information is used to activate the fluorescence detector 7. When no droplet is detected , the fluorescent detector 7 is in an inactive state; even if the fluorescent detector 7 is always in an activated state, the information of the droplet surface morphology also has other functions, such as recording the number of droplets passing through the ...
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Abstract
The invention discloses a microfluidic detection chip based on droplet experiments, which comprises a droplet channel network. The microfluidic detection chip is characterized in that the droplet channel network comprises an outlet channel, a carrier liquid channel arranged on the outlet channel, an inlet channel arranged at the end of the outlet channel, and a detection channel arranged on the other end of the outlet channel, wherein the diameter of the inlet channel is greater than that of the droplet; the inlet channel is a conical and tapers towards the outlet channel end; the diameter of the smallest end of the inlet channel is 0.9-1 time of that of the droplet; the diameter of the outlet channel is less than or equal to that of the droplet; and the diameter of the smallest end of the inlet channel is the minimum diameter among the inlet channel, outlet channel and detection channel.
Description
technical field [0001] The invention relates to a microfluidic detection chip based on a droplet experiment. Background technique [0002] Many biomedical experiments require high-throughput experiments on sample reagents. For example, in biological research and clinical applications, genetic testing can provide high-throughput for biomarker discovery and clinical diagnosis by using high-throughput target-specific reagents. quality information. [0003] Small-volume, multi-type sample detection is a development trend. However, the generation and mixing of small-volume reagents requires more complex equipment, which increases the cost of the experiment accordingly. Therefore, emerging technologies are urgently needed to realize small-volume, multi-type sample detection. samples for rapid testing. [0004] Microdroplet technology offers great prospects for high-throughput experiments. Emulsion technology can generate hundreds of millions of small-volume droplets. These mutua...
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IPC IPC(8): C12M1/34C12M1/00
CPCC12Q1/6844C12Q2565/629
Inventor 黎海文贾朋飞刘聪周武平张涛蒋克明张志强
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI