A kind of primer pair and application thereof for identifying giant centipede

Abstract

The invention discloses a primer pair for identifying Scolopendra subspinipes multilans(L.)Koch and an application of the primer pair. The primer pair disclosed by the invention for identifying or assistant-identifying Scolopendra subspinipes multilans(L.)Koch is specifically the primer pair of two single-chain DNA molecules shown in sequences 1 and 2 in a sequence table. Experiments verify that by adopting the primers disclosed by the invention, Scolopendra subspinipes multilans(L.)Koch and Scolopendra multidens Newport are quickly and accurately identified by virtue of a quick PCR method and a fluorescent staining method and a fluorescent dye method is introduced to detect a true and false identification result, so that a technical support for field utilization on medicinal material molecular identification is realized.

Description

Technical field

[0001] The invention belongs to the field of molecular biology, and relates to a primer pair for identifying the giant centipede and its application. Background technique

[0002] According to a record in the Chinese Pharmacopoeia (2010), the origin of centipedes is the dried body of the centipede Scolopendra subspinipes mutilans L. Koch. Centipede is a valuable traditional Chinese medicinal material in my country. It has the effects of extinguishing wind, relieving spasm, dredging collaterals and relieving pain, attacking toxins and dispelling stagnation. Headaches, sores, scrofula, snake and insect bites, etc., mainly produced in Hunan, Hubei, Guangxi, Yunnan and other places in my country (Wang Keqin, Fang Hong, Ye Maoxiang, etc. Centipede drug source investigation and product identification, Chinese herbal medicine, 1997, 20(9):450).

[0003] In recent years, centipede breeding has gradually emerged in my country. However, due to the uneven quality of most of th...

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