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Molecular target Gab2 and application thereof in preparing medicines and health care food for preventing and treating fatty liver and liver cancer

A technology of health food and molecular target, which is applied in the function, application and food preparation of food ingredients

Inactive Publication Date: 2015-04-29
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of Gab2 in lipid metabolism and metabolic diseases remains to be further studied.

Method used

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  • Molecular target Gab2 and application thereof in preparing medicines and health care food for preventing and treating fatty liver and liver cancer
  • Molecular target Gab2 and application thereof in preparing medicines and health care food for preventing and treating fatty liver and liver cancer
  • Molecular target Gab2 and application thereof in preparing medicines and health care food for preventing and treating fatty liver and liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Gab2 is highly expressed in the liver of mice fed with high-fat diet or alcohol, and more than 60% of human liver cancer cases can be used as a marker for the diagnosis of liver cancer.

[0050] (1). Mouse model of non-alcoholic fatty liver

[0051] Gab2 (full knockout / half knockout) mice were screened for non-alcoholic fatty liver modeling experiments. In the experiment, 12 8-week-old Gab2(- / -) male mice and 12 8-week-old Gab2(+ / -) male mice were selected, and each mouse was randomly divided into two groups, 6 in each group, respectively group and high-fat group, the control group was fed with normal mouse basal feed, and the high-fat group was fed with high-fat feed (basic feed 77.9%+ lard 10%, cholesterol 2%, egg yolk powder 10%, bile salt 0.1%). The body weight of each group was measured regularly after the modeling started to draw the growth curve, and the mice were sacrificed when the weight of the mice no longer increased significantly at 6 months aft...

Embodiment 2

[0060] Example 2: Gab2 gene knockout blocks obesity and liver fat accumulation in mice induced by high-fat diet.

[0061] Weighed the body weight regularly after the start of NAFLD modeling, and killed the mice after 6 months of modeling no longer significant weight gain, took pictures, weighed body weight, liver weight, and epididymal fat weight, and collected blood to detect serum glutamate Transaminase (ALT), triglyceride (TG), total cholesterol (TC), the whole liver was removed for pathological observation after HE staining.

[0062] 1. Knockout of Gab2 gene effectively reduces high-fat-induced obesity in mice

[0063] After 24 weeks of modeling, the mice were weighed. Gab2 (knockout / heterozygous) mice in the high-fat group were obese compared with their respective controls, and their body weight increased significantly, while the high-fat mice in the Gab2 knockout group were obese. Lighter, body weight than heterozygous mice also had a significant decrease (eg Figure 4...

Embodiment 3

[0069] Example 3: Gab2 gene knockout mice can resist acute or chronic liver injury and fat production caused by alcohol.

[0070] In addition, it was also found that acute or chronic liver injury and fat production in alcohol-induced mice were also inhibited by knockout of the Gab2 gene. Firstly, acute and chronic alcoholic fatty liver models were constructed. The acute model adopts the experimental method of force-feeding alcohol (6g / kg body weight) and maltodextrin with the same volume and heat as alcohol. The adult mice are randomly divided into four groups according to the genotype, and the alcohol and the control liquid are respectively force-fed. After 12 hours, Plasma and liver were collected. The results showed that alcohol-induced lipid droplet formation was significantly improved in Gab2-deficient mice fed alcohol (e.g. Figure 9 ).

[0071] The construction of chronic alcoholic fatty liver model According to the method of reference, the adult mice were randomly d...

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Abstract

The invention discloses molecular target Gab2 and application thereof in preparing medicines and health care food for preventing and treating fatty liver and liver cancer, and relates to a molecular target. The molecular target Gab2 is cytoplasm signal scaffolding protein mediating pathogenic factor-induced fatty liver and liver cancer; multiple disease animal models prove that in fatty liver and related diseases, the expression of the Gab2 can be induced by pathogenic factor; by collecting and integrating various signals by virtue of the Gab2, a comprehensive effect is generated, so that the development of diseases is promoted. The molecular target Gab2 can be applied to prepare medicines for preventing and treating fatty liver and liver cancer, and the molecular target Gab2 can be also used for preparing health care food for preventing and treating fatty liver and liver cancer.

Description

technical field [0001] The present invention relates to a molecular target, in particular to the molecular target Gab2 and its application in the preparation of medicines and health food for preventing and treating fatty liver and liver cancer. Background technique [0002] Non-alcoholic and alcoholic fatty liver disease is one of the major diseases that threaten human health in today's society, especially in my country. Especially in recent years, with the continuous improvement of people's living standards, the diet structure and lifestyle have changed greatly, and the incidence of "rich diseases" such as fatty liver is increasing. Both alcoholic fatty liver and non-alcoholic fatty liver involve a complex mechanism, and they are all pathological effects produced by a variety of regulatory signals. This complex regulation and diverse regulatory signals have brought great obstacles to the treatment of diseases, resulting in the treatment of a single or a small number of sig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47G01N33/68A23L1/29A23L33/00
CPCC07K14/47A23L33/17A23V2002/00G01N33/68A23V2200/308A23V2250/542
Inventor 吕忠显陈帅康宇佳李艳丽陶进程江红
Owner XIAMEN UNIV
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