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Method for enhancing worm grass fermentation biomass

A technology of fermenting organisms and Cordyceps, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low added value, environmental pollution, etc., and achieve the effects of low cost, improved immune function, and fast growth of animals

Inactive Publication Date: 2015-04-29
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, my country has become one of the largest snail breeding and consumption countries in the world. While the snail industry is developing rapidly, a large number of snail shells and viscera (accounting for about 70%) will be produced in the process of snail production and processing. These agricultural by-products are generally Directly processed into animal feed or discarded as waste, not only has relatively low added value, but also causes environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for improving the fermentation biomass of Cordyceps, comprising the following steps,

[0027] A. Cordyceps strains were inoculated on PDA slant medium, cultivated at 20°C for 7 days, inoculated in PDA liquid medium, and cultured with shaking at 20°C for 7 days to obtain liquid fermentation mycelia;

[0028] B. Get 90ml PDA liquid culture medium, liquid fermented mycelium is ground into mud with mash machine, collect mycelium, wash the mycelium that collects with sterilized water and seepage buffer respectively; Get 0.2g mycelium with Add the enzyme solution with a concentration of 0.02g / ml at a ratio of 1:3, let stand at 30°C for 3 hours, filter the supernatant, and centrifuge the supernatant for 8 minutes to obtain the protoplast solution;

[0029] C. Take 0.5ml of protoplast solution, and carry out radiation mutagenesis treatment under the condition of ice bath, and the treatment dose is 30Gy respectively; spread the mutagenized bacteria solution on a plate, ...

Embodiment 2

[0033] A method for improving the fermentation biomass of Cordyceps, comprising the following steps,

[0034] A. Inoculate the Cordyceps fungus on the PDA slant medium, cultivate it at 28°C for 7 days, inoculate it in the PDA liquid medium, and cultivate it with shaking at 28°C for 7 days to obtain liquid fermentation mycelia;

[0035]B. Get 110ml PDA liquid culture medium, liquid fermented mycelium is ground into mud with mash machine, collect mycelium, wash the mycelium that collects with sterilized water and osmosis buffer respectively; Get 0.8g mycelium with Add the enzyme solution with a concentration of 0.02g / ml at a ratio of 1:5, let it stand at 30°C for 3 hours, filter the supernatant, and centrifuge the supernatant for 15 minutes to obtain the protoplast solution;

[0036] C. Take 0.7ml of protoplast solution, and carry out radiation mutagenesis treatment under the condition of ice bath, and the treatment dose is 50Gy respectively; spread the mutagenized bacteria solu...

Embodiment 3

[0040] A method for improving the fermentation biomass of Cordyceps, comprising the following steps,

[0041] A. Inoculate the Cordyceps fungus on the PDA slant medium, cultivate it at 25°C for 7 days, inoculate it in the PDA liquid medium, and cultivate it with shaking at 25°C for 7 days to obtain liquid fermentation mycelia;

[0042] B. Take 100ml of PDA liquid medium, grind the liquid fermented mycelium into mud with a masher, collect the mycelium, wash the collected mycelium with sterilized water and 0.6M magnesium sulfate respectively; take 0.5g of mycelium Add the enzyme solution with a concentration of 0.02g / ml at a ratio of 1:4, the enzyme solution includes 0.2g of lysozyme, 10ml of isotonic solution; stand at 30°C for 3 hours, filter the supernatant, and centrifuge the supernatant 10min made the protoplast solution;

[0043] C. Take 0.6ml of protoplast solution and put it in a 1ml centrifuge tube, and carry out radiation mutagenesis treatment under the condition of i...

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Abstract

The invention relates to the technical fields of microbial genetic breeding and biological active substance production, and discloses a method for enhancing worm grass fermentation biomass. The method comprises the following steps: (1) preparing a worm grass protoplast; (2) carrying out mutagenesis on the worm grass protoplast, and regenerating the protoplast; (3) selecting the bacterial colony, and inoculating a PDA (potato dextrose agar) culture medium; (4) inoculating a liquid fermentation culture medium; and (5) obtaining the active product containing worm grass biomass, polysaccharide, cordycepin and the like. The worm grass strain with high hypha growth speed and high biomass is obtained by mutagenesis for the first time; by using white jade snail viscera and shells as the main raw material and adding proper glucose, K2HPO4, MgSO4 and other nutrient substances, the mutagenesis worm grass strain is inoculated and subjected to submerged fermentation to produce the worm grass mycelia. The method is simple to operate, sufficiently utilizes the waste resources and enhances the worm grass fermentation biomass. The fermentation product can be used in an animal feed additive to become a green food, thereby avoiding adding antibiotics in the animal feed.

Description

technical field [0001] The invention relates to the technical field of microbial genetic breeding and production of biologically active substances, in particular to a method for increasing the fermentation biomass of Cordyceps sinensis. Background technique [0002] my country's snail breeding industry is a new industry rising from the 1980s with a rapid development speed. Now it has become a hot spot in my country's specialty agricultural development and export-earning projects. In recent years, the artificial breeding of white jade snails has become mature and commercialized, and has achieved success in deep processing and comprehensive utilization, and has gradually entered the table of the general public. The vigorous development of the snail industry has greatly enriched the food baskets of urban and rural people and provided a new source of high-quality protein for the people of our country. At present, snail products have been in short supply in domestic and foreign ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12N1/14C12R1/645
Inventor 张作法吕国英石小锋金群力苗立祥范雷法
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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