Method for enhancing worm grass fermentation biomass

Abstract

The invention relates to the technical fields of microbial genetic breeding and biological active substance production, and discloses a method for enhancing worm grass fermentation biomass. The method comprises the following steps: (1) preparing a worm grass protoplast; (2) carrying out mutagenesis on the worm grass protoplast, and regenerating the protoplast; (3) selecting the bacterial colony, and inoculating a PDA (potato dextrose agar) culture medium; (4) inoculating a liquid fermentation culture medium; and (5) obtaining the active product containing worm grass biomass, polysaccharide, cordycepin and the like. The worm grass strain with high hypha growth speed and high biomass is obtained by mutagenesis for the first time; by using white jade snail viscera and shells as the main raw material and adding proper glucose, K2HPO4, MgSO4 and other nutrient substances, the mutagenesis worm grass strain is inoculated and subjected to submerged fermentation to produce the worm grass mycelia. The method is simple to operate, sufficiently utilizes the waste resources and enhances the worm grass fermentation biomass. The fermentation product can be used in an animal feed additive to become a green food, thereby avoiding adding antibiotics in the animal feed.

Description

technical field

[0001] The invention relates to the technical field of microbial genetic breeding and production of biologically active substances, in particular to a method for increasing the fermentation biomass of Cordyceps sinensis. Background technique

[0002] my country's snail breeding industry is a new industry rising from the 1980s with a rapid development speed. Now it has become a hot spot in my country's specialty agricultural development and export-earning projects. In recent years, the artificial breeding of white jade snails has become mature and commercialized, and has achieved success in deep processing and comprehensive utilization, and has gradually entered the table of the general public. The vigorous development of the snail industry has greatly enriched the food baskets of urban and rural people and provided a new source of high-quality protein for the people of our country. At present, snail products have been in short supply in domestic and foreign ...