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Rapid and sensitive detection method for plasmodium vivax

A detection method and technology for Plasmodium, applied in the field of in vitro diagnostic reagents, can solve problems such as complex operation, and achieve the effects of improving detection capability, reducing time and complexity, and rapidly and accurately detecting

Active Publication Date: 2015-04-29
SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The current gene chip detection for malaria parasite detection has been used in some researches, but the probe spotting of these gene chips mostly adopts manual mode, which is complicated to operate and increases the chance of many false positives

Method used

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  • Rapid and sensitive detection method for plasmodium vivax
  • Rapid and sensitive detection method for plasmodium vivax
  • Rapid and sensitive detection method for plasmodium vivax

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The specificity and sensitivity of the detection of embodiment 1 Plasmodium vivax

[0044] 1. Experimental steps

[0045] 1. Collection of blood samples

[0046] Take 1.0ml of whole blood sample from the patient at the port for testing.

[0047] 2. Preliminary Identification of Blood Samples

[0048] The colloidal gold method was used to preliminarily determine whether the patient was infected with Plasmodium vivax.

[0049] 3. Extraction of Genomic DNA

[0050] Plasmodium vivax DNA was extracted using a blood sample genomic DNA extraction kit (spin column type, Qiagen). Take 50uL whole blood sample, and extract DNA according to the extraction steps in the kit manual.

[0051] 4. Determination of DNA concentration

[0052] The concentration of Plasmodium DNA was quantified by real-time quantitative PCR using ABI's real-time fluorescence detector.

[0053] 5. Amplification of target genes

[0054] 5.1 Design of primers and probes

[0055] Aiming at the specific ge...

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Abstract

The invention relates to a rapid and sensitive detection method for plasmodium vivax. The detection method integrates two powerful molecular biology techniques, namely, polymerase chain reaction (PCR) and microarray, a PCR-hybridized probe is directly fixed to hybridization cabins of the microarray and the probe and a PCR reaction chamber are located at the same chip. The detection method comprises the following steps of carrying out bacteria-proliferating treatment on a sample, extracting a DNA liquid, carrying out PCR amplification, hybridizing, cleaning and interpreting the results. By the detection method, plasmodium vivax can be rapidly and sensitively detected, the detection efficiency of frontline inspection and quarantine personnel of import and export ports can be greatly improved, the workload can be reduced, the problem of possibly missed positive inspections in the traditional detection methods can be furthest solved and thus the malaria parasite epidemic situation is furthest prevented.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a rapid and sensitive detection method for Plasmodium vivax. Background technique [0002] According to WHO statistics, 107 countries and regions around the world have the prevalence and spread of malaria, 3.2 billion people are threatened by malaria, 300-500 million people get sick every year, and the death toll reaches more than 1 million. Malaria is one of the five major parasites that my country focuses on prevention and control. Malaria is a serious parasitic disease caused by Plasmodium infection. There are four species of Plasmodium that infect humans: Plasmodium falciparum (P.f), Plasmodium vivax (P.v), Plasmodium malariae (P.m) and Plasmodium ovale (P.o ). [0003] The pathogens that cause malaria in my country are mainly Plasmodium vivax and Plasmodium falciparum. Therefore, effective detection of Plasmodium is an essential means for medical dia...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12R1/90
CPCC12Q1/6837C12Q1/6893C12Q2531/113C12Q2565/501Y02A50/30
Inventor 田桢干张琳岳巧云张子龙王俐李美汤林华邱德义
Owner SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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