Identification method of chicken salmonella enteritidis inflection methylation regulatory gene

A technology of Salmonella enteritidis and Salmonella, which is applied in the field of biogenetic engineering, can solve problems such as irrelevant identification

Inactive Publication Date: 2015-04-29
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] At present, some people have compared the methylation differences between the growth rates of different chicken species, or used MEDIP technology to analyze the relationship between methylation changes and expression in the whole genome of chicken spleen after infection with patho

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  • Identification method of chicken salmonella enteritidis inflection methylation regulatory gene
  • Identification method of chicken salmonella enteritidis inflection methylation regulatory gene
  • Identification method of chicken salmonella enteritidis inflection methylation regulatory gene

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[0019] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0020] Embodiments of the present invention provide a method for identifying methylation-regulated genes infected by Salmonella chicken enteritidis, such as figure 1 As shown, the method includes the following steps:

[0021] S1. Select 2-day-old Salmonella-negative Shouguang chickens and divide them into a test group and a control group; the test group is inoculated with 0.3mL Salmonella Enteritidis bacteria solution 2-4×108cfu / bird by oral gavage, and the di...

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Abstract

The invention provides an identification method of a chicken salmonella enteritidis inflection methylation regulatory gene, and relates to the technical field of bio-genetic engineering, capable of identifying a gene taking an effect of methylation regulation in chicken salmonella enteritidis inflection. The method comprises the following steps: processing a chicken spleen tissue sample which serves as a test material, enriching methylation DNA, verifying, amplifying and purifying, and then sequencing; and identifying the gene taking an effect of methylation regulation in chicken salmonella enteritidis inflection by identifying gene methylation difference and analyzing gene functions and signal paths in accordance with change in a gene methylation difference multiple.

Description

technical field [0001] The invention relates to the technical field of biogenetic engineering, in particular to a method for identifying methylation regulation genes infected by Salmonella chicken enteritidis. Background technique [0002] Salmonella enteritidis (Salmonella enteritidis) is a gram-negative bacterium and a foodborne pathogen with great harm. After infecting poultry, it will bring serious economic losses and hidden dangers to poultry production and human health. [0003] DNA methylation is the main epigenetic modification in eukaryotes, and is an important part of epigenetics. It plays an important role in maintaining normal cell functions, genetic imprinting, embryonic development, and the occurrence of human tumors and related diseases. The occurrence of abnormal methylation is a key factor leading to diseases. The hypermethylation of CpG island (CGI) in the promoter region leads to the inactivation of immune gene expression is a hot topic in current disease...

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Application Information

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IPC IPC(8): C12Q1/68C12R1/42
CPCC12Q1/686C12Q2537/164
Inventor 李显耀王莎莎刘丽英吴桂贤
Owner SHANDONG AGRICULTURAL UNIVERSITY
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