ELISA kit for detecting syphilis specific IgM antibody and preparation method of ELISA kit
A specific and kit-based technology, applied in the field of immunoassay medical diagnosis, can solve problems such as the unavailability of syphilis IgM antibody detection kits, the monopoly of core technologies, and the difficulty in breaking through syphilis recombinant antigens.
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[0036] The present invention will be further described below in conjunction with embodiment. The following examples will help those of ordinary skill in the art to further understand the present invention, but do not limit the present invention in any form.
[0037] The methods used in the following examples are conventional methods unless otherwise specified.
[0038] The technical characteristics of the present invention are described in detail through the following examples.
[0039] The preparation of embodiment syphilis-specific IgM antibody ELISA detection kit
[0040] 1) Preparation of microplates
[0041] with carbonate buffer (recipe NaCO 3 1.6g / L, NaHCO 3 ·H 2 O3.2g / L, pH9.6) dilute goat anti-human IgM antibody, determine the optimal coating concentration of 5μg / ml by square array method, take a detachable 96-well microtiter plate, add 100μl to each well, and coat at 4°C 24 hours, then use PBST (formula KH2PO40.27g / L, Na 2 HPO 4 1.42g / L, NaCl8g / L, KCl0.2g / L, Tw...
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