ELISA kit for detecting syphilis specific IgM antibody and preparation method of ELISA kit

A specific and kit-based technology, applied in the field of immunoassay medical diagnosis, can solve problems such as the unavailability of syphilis IgM antibody detection kits, the monopoly of core technologies, and the difficulty in breaking through syphilis recombinant antigens.

Inactive Publication Date: 2015-04-29
GUANGZHOU KANGRUN BIOTECHNOLOGY CO LTD
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Problems solved by technology

However, Chinese scholars have carried out less research on the clinical detection of syphilis IgM antibody. The main reason is that imported kits are expensive, while domestic syphilis IgM antibody detection kits have not yet come out.
The main reason for the relative lag in the development of kits is that the preparation technology of syphilis recombinant antigen is backward, and the core technology is monopolized by large foreign reagent companies
Although recombinant antigen preparation technology has been successfully used in the development of TP-IgG detection kits in China, it is difficult to break through the syphilis recombinant antigen suitable for syphilis IgM detection

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  • ELISA kit for detecting syphilis specific IgM antibody and preparation method of ELISA kit

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Embodiment Construction

[0036] The present invention will be further described below in conjunction with embodiment. The following examples will help those of ordinary skill in the art to further understand the present invention, but do not limit the present invention in any form.

[0037] The methods used in the following examples are conventional methods unless otherwise specified.

[0038] The technical characteristics of the present invention are described in detail through the following examples.

[0039] The preparation of embodiment syphilis-specific IgM antibody ELISA detection kit

[0040] 1) Preparation of microplates

[0041] with carbonate buffer (recipe NaCO 3 1.6g / L, NaHCO 3 ·H 2 O3.2g / L, pH9.6) dilute goat anti-human IgM antibody, determine the optimal coating concentration of 5μg / ml by square array method, take a detachable 96-well microtiter plate, add 100μl to each well, and coat at 4°C 24 hours, then use PBST (formula KH2PO40.27g / L, Na 2 HPO 4 1.42g / L, NaCl8g / L, KCl0.2g / L, Tw...

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Abstract

The invention relates to an ELISA kit for detecting syphilis specific IgM antibody and a preparation method of the ELISA kit and belongs to the technical field of immunoassay medical diagnosis. The kit is researched by a capturing method ELISA principle and comprises an ELISA pre-coated micro-pore plate strip, a positive quality control, a negative quality control, a critical quality control, an enzyme bonder 100*, a sample diluting solution, a concentrated washing solution 20*, a substrate developing solution and a stopping solution. According to the kit, an enzyme-labeled antigen is used for replacing an enzyme-labeled antibody, and the enzyme-labeled antigen is not combined with a non-specific antibody adsorbed on an ELISA plate.

Description

technical field [0001] The invention relates to an ELISA kit for detecting syphilis-specific IgM antibody and a preparation method thereof, belonging to the technical field of immunoanalysis medical diagnosis. Background technique [0002] Syphilis is a venereal disease caused by Treponema pallidum (TP), which has extremely complex clinical manifestations and can almost involve all organs of the body. In 1999, a total of 80,406 cases of syphilis were reported nationwide, and in 2009, 327,433 cases were reported, with an annual incidence rate of 24.66 / 100,000 and an average annual increase of 14.3%; the number of reported cases of congenital syphilis increased from 109 in 1997 to 10,757 in 2009 cases, with an average annual increase of 49.2%. Guangdong Province is the peak province of syphilis, accounting for more than 10% of the total number of national reports. In 2010, 45,399 cases of syphilis were reported in the province, with a reported incidence rate of 47.10 / 100,000...

Claims

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Application Information

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IPC IPC(8): G01N33/571
CPCG01N33/571G01N33/531
Inventor 黄文喜章平谢文锋梁李新叶晶龙
Owner GUANGZHOU KANGRUN BIOTECHNOLOGY CO LTD
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