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Method for degumming magnetic immobilized phospholipase A1 in liquid-solid two-phase fluidized bed

An immobilized phospholipase, immobilized enzyme technology, applied in the direction of producing fat, fat oil/fat refining, etc., can solve the problems of high cost, difficult separation of enzyme and degummed oil, difficult separation and recovery, etc., to solve difficult separation and improve activity. and stability effects

Inactive Publication Date: 2015-05-06
NORTHEAST AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] The present invention aims at the problems that the free enzyme is not only difficult to separate and recover in the reaction system, but also generally difficult to produce, and the cost is high, and it is difficult to use repeatedly or continuously. Phospholipase A 1 method for degumming
Since the fluidized bed can realize continuous production, the present invention realizes continuous dynamic degumming, the reaction process does not require stirring, the magnetic enzyme and the liquid mobile phase can be continuously separated, and the problem of difficult separation of enzyme and degummed oil is solved

Method used

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specific Embodiment approach 1

[0006] Specific embodiment one: a magnetically immobilized phospholipase A in a liquid-solid two-phase fluidized bed 1 The method of degumming is realized through the following steps: One, magnetically immobilized phospholipase A 1 Preparation: First, lg of magnetic Fe 3 o 4 / SiOx-g-P (GMA) composite particles were placed in 50mL of phosphate buffer (0.1M, pH 7.0) and soaked for 24h. After magnetic separation, the soaked carrier was added to a certain amount of phosphoric acid containing 0.2% phospholipase buffer (0.1M, pH 7.0), while stirring the reaction at 45°C for a period of time, and finally magnetically separated and washed with phosphate buffer (0.1M, pH 7.0) to obtain the immobilized enzyme, and the obtained immobilized enzyme was placed Store in the refrigerator at 4°C for later use; 2. Magnetically immobilize phospholipase A in a liquid-solid two-phase fluidized bed 1 Degumming: Pass soybean crude oil into a magnetic fluidized bed reactor, and immobilize phosphol...

specific Embodiment approach 2

[0007] Specific embodiment two: The difference between this embodiment and specific embodiment one is that in step two, the amount of boric acid added is 0.8~1.2mL / 100g, the magnetic field strength is 0.018~0.026T, and the liquid phase velocity is 0.0020~0.0030m / s , under the condition of reaction temperature 40~65℃ and reaction time 2.8~3.8h, the degumming of magnetically immobilized phospholipase A1 in liquid-solid two-phase fluidized bed is carried out, and the phosphorus content of the degummed oil obtained under this condition is below 13mg / kg . Other steps are the same as in the first embodiment.

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Abstract

The invention provides a method for degumming magnetic immobilized phospholipase A1 (PLA1) in a liquid-solid two-phase fluidized bed. Physical refining can be realized by adopting phospholipase A1 to degum, and a nutritive value of the phospholipid also can be kept to the greatest extent. Free enzyme is not prone to separate and recycle in a reaction system, high in cost, difficult to recycle or continuously use, and therefore, the invention discloses the method for degumming magnetic immobilized phospholipase A1 in the liquid-solid two-phase fluidized bed. The magnetic immobilized phospholipase has the effects of being repeatedly used and easily recycled, so that the activity and the stability of the enzyme in the reaction system are improved. The fluidized bed can realize continuous production, and thus, the method realizes continuous dynamic degumming, is free of stirring in a reaction process, can continuously separate the magnetic enzyme from a liquid-state mobile phone, and solves a problem that the enzyme and the degummed oil are difficult to separate.

Description

technical field [0001] The invention relates to a magnetically immobilized phospholipase A in a liquid-solid two-phase fluidized bed 1 The method of degumming. Background technique [0002] In the oil processing industry, oil degumming has always been one of the focuses of people's attention. Degumming is the removal of peptized impurities in oil, and the main component is phospholipid. Phospholipids are mainly divided into hydratable phospholipids and non-hydratable phospholipids. Hydrated phospholipids can be removed by traditional hydration degumming methods, but non-hydratable phospholipids have significant hydrophobicity and are difficult to remove. The traditional degumming methods mainly include hydration degumming, adsorption degumming, acid refining degumming, enzymatic degumming, etc. to treat crude oil and reduce the content of peptized impurities in crude oil. Generally speaking, each method has its own characteristics and scope of application. According to dif...

Claims

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Application Information

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IPC IPC(8): C11B3/00
CPCC11B3/003
Inventor 刘天一于殿宇张欢江连洲王立琦刘丹怡葛洪如赵清霞
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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