Enzyme-loaded filler and preparation method and application thereof

A spraying method and sol technology, applied in the field of enzyme catalysis, can solve the problems of insufficient activity and poor stability of enzyme-loaded fillers, and solve the problems of insufficient stability and poor activity of enzyme-loaded fillers, and achieve easy industrial application, activity and stability Enhanced performance and simple preparation process

Pending Publication Date: 2019-06-04
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, the packing inside the tower body is first washed with sodium hydroxide solution to wash off the inactivated biological enzymes; then new sol enzymes are coated on the packing by bubbling from the bottom of the tower; The replacement method of the enzyme packing provides convenience, but does not give a solution to the insufficient stability and poor activity of the enzyme

Method used

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  • Enzyme-loaded filler and preparation method and application thereof
  • Enzyme-loaded filler and preparation method and application thereof
  • Enzyme-loaded filler and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0059] The schematic diagram of the preparation method of the enzyme-carrying filler of the present invention is as follows figure 1 As shown, it can be seen from the figure that the preparation method of the enzyme-carrying filler includes the following steps:

[0060] (1) Fix the packing on the mechanical rotor with wire mesh, and adjust the speed to make it rotate at a uniform speed;

[0061] (2) Use a pneumatic spray gun to spray the enzyme-containing sol on the surface of the filler on one side of the mechanical rotator, and use a hair dryer to blow dry on the other side to obtain the enzyme-carrying filler.

Example Embodiment

[0063] Example 1

[0064] The preparation method of the enzyme-carrying filler of this embodiment includes the following steps:

[0065] (1) Use metal wire mesh to fix the metal Θ ring packing on the mechanical rotor, and adjust the speed to make it rotate at a uniform speed;

[0066] (2) Use a spray gun to spray the enzyme-containing sol on the surface of the filler. The spraying process adopts zigzag spraying. In the spraying process, a blower is used to dry the surface of the metal Θ ring filler to obtain the enzyme-carrying filler. The air pressure is 0.15 MPa, the spraying flow rate is 1.82 kg / h, and the distance between the spray gun and the filler surface during the spraying is 15 cm.

[0067] The specification of the metal Θ ring packing used in this embodiment is 3 cm×3 cm, and the following examples and comparative examples all use this specification of the metal Θ ring packing.

[0068] The preparation method of the enzyme-containing sol of this embodiment includes the follo...

Example Embodiment

[0073] Example 2

[0074] The difference between this embodiment and embodiment 1 is that the spraying air pressure is replaced with 0.2 MPa, the spraying flow is replaced with 2.05 kg / h, the distance between the spray gun and the filler surface is replaced with 20 cm, and the other conditions are exactly the same as those of the example 1. .

[0075] The enzyme-containing sol used in this example is the same as in Example 1.

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Abstract

The invention relates to an enzyme-loaded filler and a preparation method and application thereof. The preparation method of the enzyme-loaded filler lies in that enzyme-containing sol is sprayed to the surface of the filler, so that the enzyme-loaded filler is obtained. Compared with the enzymic activity and the stability of an enzyme-loaded filler prepared by an impregnation method, the enzymicactivity and the stability of the enzyme-loaded filler prepared by the preparation method disclosed by the invention are obviously improved; after the enzyme-loaded filler is stored at room temperature for 50 days, the enzymic activity loss is smaller than 5%, and the enzymic activity for catalyzing the rectification process of a transesterification reaction of ethyl acetate and n-butanol is 599-621U/g.

Description

technical field [0001] The invention relates to the field of enzyme catalysis, in particular to an enzyme-carrying filler and its preparation method and application. Background technique [0002] Immobilized enzymes reduce the mobility of enzymes by physical or chemical methods. After the enzyme is immobilized, the general stability increases, it is easy to separate from the reaction system, and it is easy to control and can be used repeatedly. It is convenient for transportation and storage, and is conducive to automatic production, but the activity is reduced and the scope of use is reduced. [0003] CN105176962A discloses a method for immobilizing biological enzymes, specifically a method for immobilizing biological enzymes in ceramic fillers. The method includes: step 1, the process of treating biological enzymes; step 2, the process of treating ceramic fillers; step 3, the process of mixing the treated biological enzymes and ceramic fillers to prepare bioceramic fille...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12P7/62B05D1/02B05D3/04
Inventor 王洪海卢一凡刘文静许玉超陈二轩刘洪丽段宝轩尹依
Owner HEBEI UNIV OF TECH
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