Rana japonica oil antioxidant polypeptide

Abstract

The invention relates to a rana japonica oil antioxidant polypeptide. The rana japonica oil contains abundant nutrient substance, however in the existing using method of rana japonica oil, the nutrient substance is not likely to be absorbed by a human body due to too big molecular weight of proteins, the polypeptide with even molecular weight still cannot be obtained after rana japonica oil is subjected to enzymolysis by a conventional method, the efficient utilization of nutrient substances cannot be realized. The invention provides a rana japonica oil polypeptide with small molecular weight, which is prepared by the method specifically comprising the following steps: adjusting the pH (potential of hydrogen) of a rana japonica oil solution to 1.6-1.8, adding 0.1-0.3g / ml pepsase to carry out enzymolysis completely; adjusting the pH of the solution to 6.0-7.0, adding 0.2-0.4g / ml neutral protease to carry out enzymolysis completely; adjusting the pH of the solution to 7.0-8.0, adding bromelain to carry out enzymolysis completely; heating to 70-75 DEG C, inactivating the enzyme to obtain enzymolysis solution; filtering with a 0.3-0.4mu m filter film, and then filtering with a ultra-filtration film with molecular weight cut-off of 4-5kD, adding activated carbon, stirring, filtering, concentrating and drying, so as to obtain rana japonica oil polypeptide; the polypeptide can be easily absorbed and has an excellent antioxidant effect.

Description

technical field

[0001] The invention relates to the field of protein polypeptides, in particular to a wood frog oil antioxidant polypeptide. Background technique

[0002] Rana oil, commonly known as toad oil or snow clam ointment, "Compendium of Materia Medica" records: Hashi toad, which is called "Shanha" in "Compendium of Materia Medica", aliased as Hashi toad and snow clam. Now it is named as Chinese Rana. It mainly grows in the mountains of northeast China, including the Changbai Mountains, most of the Xiaoxing’an Mountains, and the hinterland of Zhangguangcai Mountain. It is a pure wild animal, also known as Hashma (Hashi toad). It is a unique frog species in the Northeast. It is called "snow clam oil", which has the effects of tonifying deficiency, nourishing the lungs, and strengthening the body. Since ancient times, it has been hailed as a good nourishing product handed down as famous as the three treasures of the Northeast. It has been widely used by people for th...