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Rana japonica oil antioxidant polypeptide

The technology of antioxidant polypeptide and Rana oleifera oil is applied in the field of protein polypeptide, which can solve the problems of inability to achieve efficient utilization of nutrients, uneven molecular weight of polypeptides, and low content of polypeptides, and achieve good antioxidant effect.

Inactive Publication Date: 2015-05-06
AESTHETIC TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, skin care products processed from wood frog oil are not easily absorbed by the human body due to the large molecular weight of the protein
After using the conventional one-step enzymatic hydrolysis method, the molecular weight of the obtained peptides is not uniform, and the content of small molecular weight peptides is small, and it is still impossible to achieve efficient utilization of nutrients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Prepare the antioxidant polypeptide of Rana oil according to the following steps:

[0029] 1) Take 200ml of wood frog oil, remove the fascia, add 1600ml of water, soak at 3°C ​​for 10 hours, and get wood frog oil solution;

[0030] 2) Adjusting the pH value of the Rana oil solution obtained in step 1) to 1.7, adding 0.2 g / ml pepsin with an enzyme specific activity of 4000 U / g, and enzymatically hydrolyzing it at 38° C. for 4.5 hours to obtain a pepsin hydrolyzate;

[0031] 3) Adjust the pH value of the pepsin hydrolyzate obtained in step 2) to 6.5, add 0.3 g / ml neutral protease with an enzyme specific activity of 60000 U / g, and perform enzymatic hydrolysis at 50° C. for 3.5 hours to obtain neutral proteolysis liquid;

[0032] 4) Adjust the pH value of the neutral protease hydrolyzate obtained in step 3) to 7.5, add 0.3 g / ml bromelain with an enzyme specific activity of 800,000 U / g, and enzymolyze it at 58° C. for 2.5 hours to obtain a pineapple hydrolyzate ;

[0033]...

Embodiment 2

[0036] Prepare the antioxidant polypeptide of Rana oil according to the following steps:

[0037] 1) Take 200ml of wood frog oil, remove the fascia, add 400ml of water, soak at 2°C for 8 hours, and get wood frog oil solution;

[0038] 2) Adjust the pH value of the obtained Rana oil solution in step 1) to 1.6, add 0.1 g / ml pepsin with an enzyme specific activity of 3000 U / g, and perform enzymatic hydrolysis at 35° C. for 4 hours to obtain a pepsin hydrolyzate;

[0039] 3) Adjust the pH value of the pepsin hydrolyzate obtained in step 2) to 6.0, add 0.2 g / ml neutral protease with an enzyme specific activity of 40,000 U / g, and perform enzymatic hydrolysis at 45°C for 3 hours to obtain a neutral protein hydrolyzate ;

[0040] 4) adjusting the pH value of the neutral protease hydrolyzate obtained in step 3) to 7.0, adding 0.2 g / ml bromelain with an enzyme specific activity of 600,000 U / g, and enzymatically hydrolyzing it at 55° C. for 2 hours to obtain a pineapple hydrolyzate;

...

Embodiment 3

[0044] Prepare the antioxidant polypeptide of Rana oil according to the following steps:

[0045] 1) Take 200ml of wood frog oil, remove the fascia, add 2000ml of water, soak at 4°C for 12 hours, and get wood frog oil solution;

[0046] 2) Adjust the pH value of the Rana oil solution obtained in step 1) to 1.8, add 0.3 g / ml pepsin with an enzyme specific activity of 5000 U / g, and perform enzymatic hydrolysis at 40° C. for 5 hours to obtain a pepsin hydrolyzate;

[0047] 3) Adjust the pH value of the pepsin hydrolyzate obtained in step 2) to 7.0, add 0.4 g / ml neutral protease with an enzyme specific activity of 70,000 U / g, and enzymolyze it at 55°C for 4 hours to obtain a neutral protein hydrolyzate ;

[0048] 4) adjusting the pH value of the neutral protease hydrolyzate obtained in step 3) to 8.0, adding 0.4 g / ml bromelain with an enzyme specific activity of 900,000 U / g, and enzymatically hydrolyzing it at 60° C. for 3 hours to obtain a pineapple hydrolyzate;

[0049] 5) Heat ...

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Abstract

The invention relates to a rana japonica oil antioxidant polypeptide. The rana japonica oil contains abundant nutrient substance, however in the existing using method of rana japonica oil, the nutrient substance is not likely to be absorbed by a human body due to too big molecular weight of proteins, the polypeptide with even molecular weight still cannot be obtained after rana japonica oil is subjected to enzymolysis by a conventional method, the efficient utilization of nutrient substances cannot be realized. The invention provides a rana japonica oil polypeptide with small molecular weight, which is prepared by the method specifically comprising the following steps: adjusting the pH (potential of hydrogen) of a rana japonica oil solution to 1.6-1.8, adding 0.1-0.3g / ml pepsase to carry out enzymolysis completely; adjusting the pH of the solution to 6.0-7.0, adding 0.2-0.4g / ml neutral protease to carry out enzymolysis completely; adjusting the pH of the solution to 7.0-8.0, adding bromelain to carry out enzymolysis completely; heating to 70-75 DEG C, inactivating the enzyme to obtain enzymolysis solution; filtering with a 0.3-0.4mu m filter film, and then filtering with a ultra-filtration film with molecular weight cut-off of 4-5kD, adding activated carbon, stirring, filtering, concentrating and drying, so as to obtain rana japonica oil polypeptide; the polypeptide can be easily absorbed and has an excellent antioxidant effect.

Description

technical field [0001] The invention relates to the field of protein polypeptides, in particular to a wood frog oil antioxidant polypeptide. Background technique [0002] Rana oil, commonly known as toad oil or snow clam ointment, "Compendium of Materia Medica" records: Hashi toad, which is called "Shanha" in "Compendium of Materia Medica", aliased as Hashi toad and snow clam. Now it is named as Chinese Rana. It mainly grows in the mountains of northeast China, including the Changbai Mountains, most of the Xiaoxing’an Mountains, and the hinterland of Zhangguangcai Mountain. It is a pure wild animal, also known as Hashma (Hashi toad). It is a unique frog species in the Northeast. It is called "snow clam oil", which has the effects of tonifying deficiency, nourishing the lungs, and strengthening the body. Since ancient times, it has been hailed as a good nourishing product handed down as famous as the three treasures of the Northeast. It has been widely used by people for th...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 陈光赵阳赵云霞
Owner AESTHETIC TECH BEIJING
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