Method for screening saline-alkaline resistant/tolerant cotton

A saline-alkali, cotton-resistant technology, applied in the field of plant physiology, can solve the problem of lack of a good evaluation method, and achieve the effects of stable identification results, strong practicability and operability, and short duration

Inactive Publication Date: 2015-05-13
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is still no good evaluation method for the target traits of new cotton materi...

Method used

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  • Method for screening saline-alkaline resistant/tolerant cotton

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example 1

[0021] 1. Experimental method:

[0022] (1) Prepare cotton culture solution according to the 1 / 2 Hoagland complete nutrient solution formula, and adjust the pH value of the nutrient solution between 6.5-7.0;

[0023] (2) Take the culture medium of step (1) as a solution, prepare respectively NaCl solutions of 0.0% (CK), 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6% and 0.8%, and pack into In a transparent glass bottle with a height of 60mm;

[0024] (3) Adopt "sand soil germination method", when the cotton seeds germinate and grow until the cotyledons are fully expanded and become green and no fibrous roots appear, the roots of the cotton seedlings are rinsed with clean water in the drain basin, and the individual plants are moved into the step (2) ) is configured and packed into glass bottles of different NaCl solutions, and the cotton cotyledon stays outside the bottle mouth;

[0025] (4) Wrap a layer of tight tin foil paper or light-shielding material on the outside of the glass bo...

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Abstract

The invention relates to the field of plant physiology, in particular to a method for screening saline-alkaline resistant/tolerant cotton. The method comprises the steps that (1), a cotton culture solution is prepared, and the pH value of the cotton culture solution is adjusted between 6.5 and 7.0; (2), the culture solution serves as a solution to prepare NaCl solutions with the concentration being 0.0 wt%, 0.1 wt%, 0.2 wt%, 0.3 wt%, 0.4 wt%, 0.5 wt%, 0.6 wt% and 0.8 wt%; (3), a sand sprout method is adopted, when cotton seeds sprout and grow until all cotyledons are spread and turn into green and no root is generated, the cotton seeds are moved in the NaCl solutions; (4), cultivation is carried out in a shading condition; (5), in the ninth to tenth day, seedlings are taken out for surveying the root hair number of each cotton seedling, and the saline-alkaline resistant/tolerant degree of cotton is judged according to the number of the root hairs. The method excludes the interference of the factors that salinity gradient is uneven and rainfall wets salt in the field identification process, the duration is short, the identification result is stable and reliable, and method is high in practicability and maneuverability.

Description

technical field [0001] The invention relates to the field of plant physiology, in particular to a screening method for cotton resistance / salt-alkali tolerance. Background technique [0002] my country has a large area of ​​saline-alkali land. With the continuous deterioration of the ecological environment, including industrial modernization, the expansion of irrigated land and protected land cultivation area, the area of ​​secondary salinization of soil is increasing year by year. Soil salinization and shortage of fresh water resources have become a worldwide problem. Cotton is an important economic crop in my country and a pioneer crop planted in saline-alkali land. The development of inland and coastal saline-alkali land to plant cotton is in line with the development principle of "not competing with grain and cotton for land" ,with broadly application foreground. [0003] With the rapid development of my country's agricultural transgenic technology, with the support of my...

Claims

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Application Information

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IPC IPC(8): A01G7/00A01G7/06
CPCA01G7/06A01G22/00
Inventor 雒珺瑜崔金杰张帅吕丽敏王春义朱香镇李春花
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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