Method for screening saline-alkaline resistant/tolerant cotton

Abstract

The invention relates to the field of plant physiology, in particular to a method for screening saline-alkaline resistant / tolerant cotton. The method comprises the steps that (1), a cotton culture solution is prepared, and the pH value of the cotton culture solution is adjusted between 6.5 and 7.0; (2), the culture solution serves as a solution to prepare NaCl solutions with the concentration being 0.0 wt%, 0.1 wt%, 0.2 wt%, 0.3 wt%, 0.4 wt%, 0.5 wt%, 0.6 wt% and 0.8 wt%; (3), a sand sprout method is adopted, when cotton seeds sprout and grow until all cotyledons are spread and turn into green and no root is generated, the cotton seeds are moved in the NaCl solutions; (4), cultivation is carried out in a shading condition; (5), in the ninth to tenth day, seedlings are taken out for surveying the root hair number of each cotton seedling, and the saline-alkaline resistant / tolerant degree of cotton is judged according to the number of the root hairs. The method excludes the interference of the factors that salinity gradient is uneven and rainfall wets salt in the field identification process, the duration is short, the identification result is stable and reliable, and method is high in practicability and maneuverability.

Description

technical field [0001] The invention relates to the field of plant physiology, in particular to a screening method for cotton resistance / salt-alkali tolerance. Background technique [0002] my country has a large area of ​​saline-alkali land. With the continuous deterioration of the ecological environment, including industrial modernization, the expansion of irrigated land and protected land cultivation area, the area of ​​secondary salinization of soil is increasing year by year. Soil salinization and shortage of fresh water resources have become a worldwide problem. Cotton is an important economic crop in my country and a pioneer crop planted in saline-alkali land. The development of inland and coastal saline-alkali land to plant cotton is in line with the development principle of "not competing with grain and cotton for land" ,with broadly application foreground. [0003] With the rapid development of my country's agricultural transgenic technology, with the support of my...

AI Technical Summary

Problems solved by technology

There is still no good evaluation method for the target traits of new cotton materials that have emerged, such as the performance and degree of resistance to salt and alkali

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