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Variants having glucoamylase activity

A technology of glucoamylase and variant, applied in the direction of glycosylase, enzyme, hydrolase, etc.

Inactive Publication Date: 2015-05-13
DUPONT NUTRITION BIOSCIENCES APS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason this method is not commonly used is that active enzyme can then be present in the final product, which is undesirable as stated above

Method used

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  • Variants having glucoamylase activity
  • Variants having glucoamylase activity
  • Variants having glucoamylase activity

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0075] Glucoamylases are commercially important enzymes in a wide range of applications requiring the hydrolysis of starch. Disclosed herein are glucoamylase variants with reduced thermostability for hydrolyzing starch. These glucoamylase variants contain amino acid substitutions in the catalytic domain and / or the starch binding domain. Said variants exhibit altered properties, such as altered thermostability and / or altered specific activity.

[0076] Furthermore, it is described herein that certain subsets of glucoamylase variants are very useful to add to fermentation vessels, for example, during beer fermentation, since the appropriate thermolabile nature of the enzyme allows inactivation by pasteurization become possible.

[0077] Pasteurization experiments have been performed on beer at laboratory scale, pilot scale and full scale to assess the ability to inactivate the variants described herein during brewing. Lab-scale pasteurization was demonstrated on bottled beer ...

Embodiment 1

[0451] Example 1: A glucoamylase variant comprising an interfacial amino acid group consisting of residues 29, 43, 48, 116 and 502 of SEQ ID NO: 2 or equivalent positions in the parent glucoamylase and in the set of catalytic core amino acid residues consisting of residues 97, 98, 147, 175, 483 and 484 of SEQ ID NO: 2 or equivalent positions in the parent glucoamylase One, two or three amino acid substitutions.

Embodiment 2

[0452] Embodiment 2: A glucoamylase variant comprising

[0453] a) Amino acid substitution at the residue corresponding to position 502 of SEQ ID NO: 2 or the equivalent position in the parent glucoamylase, and optionally, residues 29, 43 selected from SEQ ID NO: 2 , 48 and 116, or the amino acid substitution of the interface amino acid group consisting of equivalent positions in the parent glucoamylase;

[0454] b) amino acid substitutions at the residue corresponding to position 98 of SEQ ID NO: 2 or the equivalent position in the parent glucoamylase, and optionally, residues 97, 147 selected from SEQ ID NO: 2 , 175, 483, and 484, or one or two amino acid substitutions in the catalytic core amino acid residue set consisting of equivalent positions in the parent glucoamylase;

[0455] The glucoamylase variant has at least one amino acid substitution selected from the group of interface amino acid residues or the group of catalytic core amino acid residues;

[0456] wherein ...

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Abstract

The present invention relates to variants having glucoamylase activity with improved properties and to compositions comprising these variants suitable for use for example in the production of a food, beverage (e.g. beer), feed, biochemical, or biofuel. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells. Furthermore, different methods and uses related to glucoamylases according to the invention are disclosed, such as in a brewing process.

Description

[0001] References to Sequence Listings [0002] Attached is a Sequence Listing comprising SEQ ID NO: 1-31, which is hereby incorporated by reference in its entirety. technical field [0003] The present invention relates to variants having glucoamylase activity with improved properties and to compositions comprising these variants suitable eg for the production of food, beverages (eg beer), feed, biochemicals or biofuels. The invention also discloses a DNA construct encoding the variant and a method for producing the glucoamylase variant in a host cell. Furthermore, different methods and uses are disclosed in relation to the glucoamylases according to the invention, eg in brewing processes. Background technique [0004] Glucoamylases (glucan 1,4-alpha-glucohydrolase, EC 3.2.1.3) are starch hydrolytic exocarbases that catalyze the removal of non-reducing ends from starch or related oligo- and polysaccharide molecules. In addition to consecutive glucose units. Glucoamylases...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/34
CPCC12N9/2428C12Y302/01003C12C7/04C12C11/00Y02E10/30
Inventor J.F.克拉梅P.E.德格恩I.尼科拉伊V.阿勒克塞耶夫C.W.维罗伊曼D.托雷斯帕兹米诺N.M.菲什
Owner DUPONT NUTRITION BIOSCIENCES APS
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