A kind of aspergillus niger seed cultivation method

A culture method, the technology of Aspergillus niger spores, which is applied in the field of biological fermentation, can solve the problems that the growth stage cannot be guaranteed, the fermentation result is affected, and it is difficult to truly reflect the growth state of Aspergillus niger, and the timeliness is good, the consumption is low, and the measurement is simple. convenient effect

Active Publication Date: 2020-10-02
JIANGSU GUOXIN UNION ENERGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is difficult to truly reflect the growth state of Aspergillus niger, and due to the differences between batches, the cultivation time is used to determine the transplantation, resulting in different growth stages of seeds between different batches, and it cannot guarantee that they are in a state with high vigor. Growth stage, thus affecting the subsequent fermentation results

Method used

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  • A kind of aspergillus niger seed cultivation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] After the corn is crushed, pass through a 80-mesh sieve; the obtained corn flour and tap water are uniformly mixed according to a material-to-water ratio of 1:3, the pH of the slurry is adjusted to 5.8, and high-temperature α-amylase is added in an amount of 20 U / g corn flour; The obtained powder slurry is sprayed twice, and the iodine test turns light brown to obtain a qualified liquefied liquid.

[0034] The liquefied solution was diluted with tap water, and ammonium sulfate was added to prepare a seed medium with 10% total sugar and 0.2% total nitrogen. After sterilizing at 121°C for 20 minutes and cooling down to 37°C, the spore suspension of Aspergillus niger CICC 40021 (strain source: CICC preserved strain) was inoculated, so that the concentration of spores after inoculation was 300,000 / ml.

[0035] The seed culture conditions are: temperature 37°C, dissolved oxygen concentration and stirring speed coupling feedback control, and the dissolved oxygen solubility is...

Embodiment 2

[0038] After the corn is crushed, pass through a 80-mesh sieve; the obtained corn flour and tap water are uniformly mixed according to a material-to-water ratio of 1:3, the pH of the slurry is adjusted to 5.8, and high-temperature α-amylase is added in an amount of 20 U / g corn flour; The obtained powder slurry is sprayed twice, and the iodine test turns light brown to obtain a qualified liquefied liquid.

[0039] The liquefied solution was diluted with tap water, and ammonium sulfate was added to prepare a seed medium with 10% total sugar and 0.2% total nitrogen. After sterilizing at 121°C for 20 minutes and cooling down to 37°C, the spore suspension of Aspergillus niger CICC 40021 (strain source: CICC preserved strain) was inoculated, so that the concentration of spores after inoculation was 300,000 / ml.

[0040] The seed culture conditions are: temperature 37°C, dissolved oxygen concentration and air volume coupling feedback control, and the dissolved oxygen solubility is con...

Embodiment 3

[0043] After the corn is crushed, pass through a 80-mesh sieve; the obtained corn flour and tap water are uniformly mixed according to a material-to-water ratio of 1:3, the pH of the slurry is adjusted to 5.8, and high-temperature α-amylase is added in an amount of 20 U / g corn flour; The obtained powder slurry is sprayed twice, and the iodine test turns light brown to obtain a qualified liquefied liquid.

[0044] The liquefied solution was diluted with tap water, and ammonium sulfate was added to prepare a seed medium with 12% total sugar and 0.4% total nitrogen. After sterilizing at 121°C for 20 minutes and cooling down to 38°C, inoculate the spore suspension of Aspergillus niger CICC 40021 (strain source: CICC preserved strain), so that the concentration of spores after inoculation is 400,000 / ml.

[0045] The seed culture conditions are: temperature 38°C, dissolved oxygen concentration and stirring speed coupling feedback control, and the dissolved oxygen solubility is contr...

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Abstract

The invention provides a culture method of Aspergillus niger seeds. The transplantation opportunity is selected by monitoring the acid production speed, the glucose consumption speed or the glucoamylase activity in the culture process. The invention specifically discloses a method for determining the acid production speed, the glucose consumption speed or the glucoamylase activity and discloses influence of the concentration of dissolved oxygen on the culture process. The defect that the growth state of the seeds is judged mainly by observing the sizes and shapes of Aspergillus niger balls in a conventional culture method is overcome. The standard deviation of fermentation results of the seeds cultured with the method is smaller, the seed fermentation in adjacent batches is more stable, the fermentation conversion rate is increased, and the fermentation period is remarkably shortened.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for cultivating Aspergillus niger seeds. Background technique [0002] Citric acid is currently the organic acid with the largest output and consumption, and is widely used in industries such as beverage, food and medicine. my country is a major producer of citric acid, with a total production capacity of more than 1 million tons. Domestic citric acid production is based on Aspergillus niger as the fungus and submerged liquid fermentation with corn, cassava and other starchy materials as raw materials, generally using secondary fermentation. Before carrying out fermentation to prepare citric acid, primary seed culture is usually carried out to obtain a large number of Aspergillus niger mycelia with high vigor and strong acid production ability. [0003] During the growth process of microorganisms, the cell viability is usually higher in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12Q3/00C12P7/48C12R1/685
CPCC12N1/14C12P7/48C12Q3/00
Inventor 石贵阳陈坚胡志杰李江华蒋小东彭艳红金赛孙福新王宝石王莉
Owner JIANGSU GUOXIN UNION ENERGY CO LTD
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