Bifidobacterium bifidum strain TMC3115 with fat suppression cells and application therepof
A technology of Bifidobacterium bifidum and macrophages, which can be used in bacteria, applications, dairy products, etc., and can solve problems such as complex interactions
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Embodiment 1
[0040] Embodiment 1, Bifidobacterium bifidum milk drink
[0041] Skim milk 10%-15% fortified with fresh milk or concentrated skim milk or skim milk powder. Sugar solution: sweetener (sugar or glucose or other commonly used sweeteners) 22%-30%, citric acid 0.05%-0.1%, stabilizer (any stabilizer commonly used in the field for dairy products) 0.1%- 0.4%. The ratio of fermentation broth, sugar solution and water is 1:2:1.
[0042] Mix fresh milk or concentrated skim milk or skim milk powder fortified with 40-50°C deionized water, fully dissolve, preheat to 60-65°C, homogenize under 20MPa pressure, and heat at 90-95°C Heat and sterilize under low temperature for 5-30 minutes, cool to 42°C; then add 0.003%-0.01% of starter and 0.002%-0.004% of Bifidobacterium bifidum TMC3115 bacteria powder, and cultivate at 42°C to make the pH value 4.2-4.5, the number of viable bacteria reaches l0 8 cfu / mL or more.
[0043] In addition, the sweeteners, stabilizers, pigments, etc. used as auxi...
Embodiment 2
[0044] Embodiment 2, Bifidobacterium bifidum yogurt
[0045] Heat the fresh milk to above 50°C, add 6.5-8% white sugar and stir until completely dissolved, preheat to 60-65°C, homogenize with 20Mpa pressure, sterilize at about 95°C for 5 minutes, cool to 42-45°C, inoculate and ferment Agent 0.003%-0.01% and Bifidobacterium bifidum 0.002%-0.004% of the present invention. Ferment at 40-42°C to pH 4.2-4.5, cool and refrigerate to make set or stirred yogurt.
Embodiment 3
[0046] Embodiment 3, Bifidobacterium bifidum ice cream
[0047] Prepare 1000kg ice cream, use white granulated sugar 75kg, whole milk powder 66kg, butter 32kg, coconut oil 23kg, syrup 26.5kg, stabilizer 6kg, Bifidobacterium bifidum bacterium powder 0.002%-0.004% of the present invention, will remove bacteria powder Mix other materials, preheat to 60-65°C, homogenize at 20Mpa, sterilize at 70-85°C for 10-30 minutes, cool to 40-50°C, add Bifidobacterium bifidum bacteria powder of the present invention, and sterilize at 20-30Mpa Homogenize, place at 2-4°C for 6 hours, freeze and harden to make ice cream.
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