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Bifidobacterium microcapsule and preparing method thereof

The technology of bifidobacteria and microcapsules is applied in the field of preparation of probiotic products, which can solve the problems of intolerance to gastric acid and the decrease of viable bacteria content, and achieve the effects of short storage period, simple preparation method and good storage stability.

Active Publication Date: 2011-10-12
SHAANXI GIANT BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a bifidobacterium microcapsule and its preparation method which can effectively prolong the storage period of bifidobacteria, which solves the problems that the content of viable bacteria in the liquid bifidobacterium preparation is greatly reduced and the problem of gastric acid intolerance

Method used

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  • Bifidobacterium microcapsule and preparing method thereof
  • Bifidobacterium microcapsule and preparing method thereof
  • Bifidobacterium microcapsule and preparing method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Step 1: Under the temperature condition of 37°C, anaerobically culture the activated bifidobacterium in the liquid medium for 24 hours, collect the bacteria by centrifugation, wash with 0.85% normal saline to prepare the bacteria suspension;

[0047] Step 2: Prepare a protective agent, add 1.23 g of animal-derived collagen, 11.50 g of trehalose, and 4.65 mL of glycerin to each 1000 mL of protective agent, and the rest is distilled water;

[0048] Step 3: Mix the sterilized protective agent and bacterial suspension evenly at a volume ratio of 2:1, let stand for 30 minutes, pre-freeze at -20°C for 2 hours, and then store at -50°C under vacuum The temperature is 50 millitorr and dried in a vacuum freeze-drying oven for 48 hours to prepare freeze-dried bacteria powder;

[0049]Step 4: Add 0.1 g of freeze-dried bacteria powder to the sodium alginate solution with a mass fraction of 1%, the mass ratio of freeze-dried bacteria powder to sodium alginate is 1:3, and mix well to ...

Embodiment 2

[0053] Step 1: Under the temperature condition of 37°C, anaerobically culture the activated bifidobacterium in the liquid medium for 24 hours, collect the bacteria by centrifugation, wash with 0.85% normal saline to prepare the bacteria suspension;

[0054] Step 2: prepare a protective agent, add 1.23 g of human-like collagen, 11.50 g of trehalose, and 4.65 mL of glycerin per 1000 mL of protective agent, and the rest is distilled water;

[0055] Step 3: Mix the sterilized protective agent and bacterial suspension evenly at a volume ratio of 2:1, let stand for 30 minutes, pre-freeze at -20°C for 2 hours, and then store at -50°C under vacuum The temperature is 75 millitorr and dried in a vacuum freeze-drying oven for 48 hours to prepare freeze-dried bacteria powder;

[0056] Step 4: Add 0.1 g of freeze-dried bacteria powder to the sodium alginate solution with a mass fraction of 1.5%, the mass ratio of freeze-dried bacteria powder to sodium alginate is 1:4, and mix well to obtai...

Embodiment 3

[0060] Step 1: Under the temperature condition of 37°C, anaerobically culture the activated bifidobacterium in the liquid medium for 24 hours, collect the bacteria by centrifugation, wash with 0.85% normal saline to prepare the bacteria suspension;

[0061] Step 2: prepare a protective agent, add 1.23 g of human-like collagen, 11.50 g of sucrose, and 4.65 mL of glycerin per 1000 mL of protective agent, and the rest is distilled water;

[0062] Step 3: Mix the sterilized protective agent and bacterial suspension evenly at a volume ratio of 2:1, let stand for 30 minutes, pre-freeze at -20°C for 2 hours, and then store at -50°C under vacuum The temperature is 100 millitorr and dried in a vacuum freeze-drying oven for 48 hours to prepare freeze-dried bacteria powder;

[0063] Step 4: Add 0.1 g of freeze-dried bacteria powder to the sodium alginate solution with a mass fraction of 2%, the mass ratio of freeze-dried bacteria powder to sodium alginate is 1:5, and mix well to obtain t...

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Abstract

The invention specifically relates to a bifidobacterium microcapsule and a preparing method thereof. The bifidobacterium is obligatorily anaerobic and very sensitive to oxygen, PH, temperature, humidity, and other adverse external environment, thereby being very hard to remain activity during production, storage and transport; besides, if taken orally in a form of dry bifidobacterium powder, the bifidobacterium cannot tolerate low-pH value gastric acid, bile salt, and other environments, so a purpose that massive survived bifidobacteria arrive at an intestinal tract and colonise on the intestinal mucosa is hard to be guaranteed. The preparing method disclosed by the invention comprises the following steps of: adding freeze-dried bifidobacterium powder into a sodium alginate solution and then mixing in soybean oil, emulsifying the mixed solution and standing; centrifugally collecting micro-capsules; and drying the micro-capsules by using a vacuum freeze-drying technology. The bifidobacterium microcapsule disclosed by the invention has the advantages of gastric acid resistance, bile salt resistance and entericsolubility, high survival rate of bifidobacterium, simple preparation method, strong practicality, convenience for industrial production, and excellent storage stability and solves the problem of short storage period of probiotics preparations.

Description

technical field [0001] The invention belongs to the technical field of preparation of probiotic products, and in particular relates to a bifidobacterium microcapsule and a preparation method thereof. Background technique [0002] Bifidobacteria are important probiotics in the intestinal tract of humans and animals. They have the properties of regulating the balance of intestinal flora, providing nutrition, lowering cholesterol and anti-cancer. However, it is obligately anaerobic and is extremely sensitive to adverse external environments such as oxygen, pH, temperature, and humidity. It is difficult to maintain activity during production, storage, and transportation; and when the dried bacteria powder is taken orally, it cannot tolerate low pH values. It is difficult to ensure that a large number of surviving bacteria reach the intestinal tract and colonize the intestinal mucosa. Contents of the invention [0003] The object of the present invention is to provide a bifido...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K35/74A61P1/00A61P3/06A61P35/00A61K35/745
Inventor 范代娣朱晓丽段志广马晓轩严建亚
Owner SHAANXI GIANT BIOTECHNOLOGY CO LTD
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