Composite premix for pig
A compound premix, premix technology, applied in the application, animal feed, animal feed and other directions, can solve the problem that the yeast C. Stability and other issues to ensure intestinal health, reduce morbidity, and improve feed conversion rate
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Embodiment 1
[0013] Stress resistance and biological performance measurement of embodiment 1 3 bacterial strains
[0014] 3 strains: Enterococcus faecalis CGMCC No.2386, Bacillus licheniformis CGMCC No.2383, Saccharomyces spp. CGMCC No.2387 were isolated and bred by the applicant from the intestinal tract or feces of healthy animals, and preserved in 4°C refrigerator.
[0015] 1) Preparation of the culture of Bacillus licheniformis CGMCC No.2383: Inoculate the slant strains stored in the refrigerator into BPY seed medium for activation, and cultivate at 37° C. and 200 rpm for 18 hours to obtain a culture with a spore rate of more than 95%;
[0016] 2) Preparation of Enterococcus faecalis CGMCC No.2386 culture: inoculate the slant bacteria stored in the refrigerator into the MRS seed medium for activation, and culture at 35°C and 100rpm for 16h to obtain the product;
[0017] 3) Preparation of the culture of the yeast CGMCC No.2387: inoculate the slant strain stored in the refrigerator int...
Embodiment 2
[0046] Embodiment 2, the preparation of compound probiotics
[0047] 1. Preparation of Bacillus licheniformis CGMCC No.2383 bacterial powder
[0048] 1) Plate culture rejuvenation: Inoculate the Bacillus licheniformis strain on the BPY plate medium, culture at 37°C for 24 hours, rejuvenate the Bacillus licheniformis, and form a single colony, pick a single colony and inoculate it on the slant medium, Incubate at 37°C for 36 hours;
[0049] 2) Preparation of first-class seeds: transfer the Bacillus licheniformis cultured in step 1) to eggplant bottle slant medium, and cultivate them at 37° C. for 12 hours to make them in the late logarithmic period to obtain first-class seeds;
[0050] Described slant medium is BPY solid medium;
[0051] 3) Preparation of secondary seeds: make bacterial suspension from the primary seeds prepared in step 2) with sterile water, and inoculate into 1.6M 3 2M of BPY seed medium 3 In the seed tank, the temperature is 37°C, the rotation speed is 2...
Embodiment 3
[0073] The preparation of embodiment 3 premix
[0074] 1. Preparation of multivitamin premix
[0075] The formula of multivitamin premix is as follows: vitamin A 3g, vitamin D3 0.8g, vitamin E 15g, vitamin K3 0.8g, vitamin B1 0.8g, vitamin B2 1.8g, vitamin B6 0.7g, vitamin B12 0.7g, niacin 8g , D-calcium pantothenate 4g, biotin 0.4g, antioxidant 0.2g, rice bran (carrier) 63.8g, mixed to make multivitamin premix;
[0076] 2. Preparation of compound trace element premix
[0077] The formula of the composite trace element premix is as follows: copper sulfate 100g, ferrous sulfate 80g, zinc sulfate 60g, manganese sulfate 40g, potassium iodide premix 10g, sodium selenite premix 4g, bentonite 106g, mixed to make a composite trace element Elemental premixes;
[0078] 3. Preparation of premix
[0079] The premix formula is as follows: 100g of the compound vitamin premix prepared above, 400g of the compound trace element premix, 200g of the compound probiotics prepared in Examp...
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