Isolation medium for radix zanthoxyli endophytic fungi

A technology for separating culture medium and endophytic fungi, which is applied in the direction of fungi, etc., can solve the problems that there are no public literature reports on the application of endophytic fungi in Liangmianzhen, and achieve the effect of long-term preservation and good stability of the bacteria

Active Publication Date: 2015-06-03
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no public literature report on the research and appl

Method used

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  • Isolation medium for radix zanthoxyli endophytic fungi
  • Isolation medium for radix zanthoxyli endophytic fungi

Examples

Experimental program
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Example Embodiment

[0015] Example 1

[0016] 16g of needle powder, 60g of corn flour, 11g of glucose, 1g of yeast extract, 0.8g of inorganic salt, 20g of agar powder, and 1L of distilled water.

[0017] Add 1 L of distilled water to the powder of needles and corn flour, and boil for 0.5 hours. Add the specified amount of glucose, yeast extract, inorganic salt, and agar powder to the filtered filtrate in turn, and finally make up 1 L with distilled water, and place in a high-pressure steam sterilizer. Under the condition of 121°C (pressure 0.1MPa), sterilize for 30 minutes and take it out for use.

Example Embodiment

[0018] Example 2

[0019] 58g of needle leaf powder, 15g of corn flour, 19g of glucose, 2g of yeast extract, 2g of inorganic salt, 20g of agar powder, and 1L of distilled water.

[0020] Add 1 L of distilled water to the powder of needles and corn flour, and boil for 0.5 hours. Add the specified amount of glucose, yeast extract, inorganic salt, and agar powder to the filtered filtrate in turn, and finally make up 1 L with distilled water, and place in a high-pressure steam sterilizer. Under the condition of 121°C (pressure 0.1MPa), sterilize for 30 minutes and take it out for use.

Example Embodiment

[0021] Example 3

[0022] 30g of needle powder, 30g of corn flour, 15g of glucose, 1.2g of yeast extract, 1.4g of inorganic salt, 20g of agar powder, and 1L of distilled water.

[0023] Add 1 L of distilled water to the powder of needles and corn flour, and boil for 0.5 hours. Add the specified amount of glucose, yeast extract, inorganic salt, and agar powder to the filtered filtrate in turn, and finally make up 1 L with distilled water, and place in a high-pressure steam sterilizer. Under the condition of 121°C (pressure 0.1MPa), sterilize for 30 minutes and take it out for use.

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Abstract

The invention discloses an isolation medium for radix zanthoxyli endophytic fungi. The isolation medium consists of radix zanthoxyli branch leaf powder, corn flour, glucose, potassium nitrate, inorganic salt, agar powder and water. A preparation method comprises the following steps: adding the radix zanthoxyli branch leaf powder and the corn flour into 1L of distilled water, and boiling for 0.5h; sequentially adding filter liquor into a specified amount of glucose, a specified amount of a yeast extract, a specified amount of inorganic salt and a specified amount of agar powder; finally supplementing the distilled water till the content is 1L, and then performing sterilization for later use. The isolation medium remarkably increases the quantity of the radix zanthoxyli endophytic fungi which are obtained by isolation and has the advantage of long strain storage time.

Description

technical field [0001] The invention relates to the technical field of microorganism culture, in particular to a culture medium for separating endophytic fungi of Liangmianzhen. Background technique [0002] Plant endophytic fungi refer to fungi that part or all of their life cycle exist in the tissues and organs of plants, which can be proved by the method of isolating from strictly surface-sterilized plant tissues or amplifying DNA from plant tissues , and the infected host plants show no outward symptoms. In 1993, American scientists isolated an endophytic fungus producing paclitaxel from the medicinal yew tissue, which aroused a research boom in the development of plant endophytic fungi and their active substances. With the deepening of research, people also found that In addition to producing the same active ingredients as the host plant, the endophytic fungi of some plants can also produce different active ingredients from the host plant. In addition, the endophytic f...

Claims

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Application Information

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IPC IPC(8): C12N1/14
CPCC12N1/14
Inventor 朱华梁子宁陈龙梁丹黄小燕
Owner GUANGXI UNIV OF CHINESE MEDICINE
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