A method for the simultaneous determination of four aflatoxins in tobacco and tobacco products based on liquid chromatography-tandem mass spectrometry

A technique of aflatoxin and tandem mass spectrometry, which is applied in the field of determination of tobacco and tobacco products, can solve the problems of simultaneous detection of multiple aflatoxins, high purity requirements of the components to be tested, complicated method operations, etc., and achieve high extraction Purification effect, increase the recovery rate of standard addition, and realize the effect of extraction and separation
CN104678039BActive Publication Date: 2016-08-24CHINA TOBACCO HUNAN INDAL CORP +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA TOBACCO HUNAN INDAL CORP
Publication Date
2016-08-24

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Abstract

The invention discloses a method for simultaneously measuring the contents of four aflatoxins in tobaccos and tobacco products by liquid chromatography-tandem mass spectrometry combination. The method comprises the steps of firstly, carrying out pretreatment such as drying and crushing on tobaccos or a cut tobacco sample, and then extracting the aflatoxins B1, B2, G1 and G2 in the tobaccos or the cut tobacco sample; purifying extract by adopting an immunoaffinity column; establishing a quantitative analysis standard curve, and detecting by using a liquid chromatography-tandem mass spectrometry combination method. After the method is used, the four aflatoxins B1, B2, G1 and G2 can be measured at the same time; furthermore, the method has the advantages of being high in sensitivity, low in limit of quantitation, high in recovery rate, good in repeatability, simple and convenient to operate, etc.
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Description

technical field

[0001] The invention relates to a method for simultaneously determining the contents of four aflatoxins in tobacco and tobacco products based on liquid chromatography-tandem mass spectrometry, and belongs to the technical field of tobacco and tobacco product determination. Background technique

[0002] In the process of tobacco primary processing, storage and transportation, tobacco factory production and tobacco product sales, due to improper storage and storage, mildew may occur in tobacco or tobacco products, which not only causes major economic losses, but also endangers the safety of cigarette products sex. Therefore, the tobacco industry attaches great importance to the control of mildew in tobacco and tobacco products.

[0003] The metabolites produced by tobacco mildew mainly include mycotoxins such as aflatoxins. There are more than 20 kinds of aflatoxins, among which aflatoxins B1, B2, G1 and G2 are the four most important.

[0004] At present, m...

Claims

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