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Tissue culture method for Eucommia ulmoides Oliv.

A tissue culture and culture medium technology, applied in the field of plant tissue culture, can solve the problems of inability to meet large-scale production of Eucommia seedlings, low germination rate of seeds, and unfavorable promotion of fine Eucommia varieties.

Inactive Publication Date: 2015-06-10
杨业云
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Eucommia seedlings are mainly propagated by seed sowing method, which is far from meeting the needs of large-scale production for Eucommia seedlings due to the low germination rate of the seeds.
Moreover, there are differences in the effective ingredients and the content of eucommia gum among the offspring of seeds, which is not conducive to the promotion of excellent varieties of eucommia.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Bud induction culture: take the stem section of Eucommia ulmoides No. 5 with buds as explants, cut off the leaves, cut into 3.0-5.0 long segments with 2-3 axillary buds, and soak them in washing powder solution for 5 minutes , and then gently brush the surface of the explant with a soft brush to remove the dust and some bacteria on the surface, then rinse it with tap water for 1 hour, then place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 5 seconds, and then wash it with sterile water for 3 seconds Then sterilize with 0.1% mercuric chloride solution for 10 minutes, rinse with sterile water for 4 times, then dry the water drops on the surface with sterile filter paper, and then inoculate into bud induction medium for bud induction. Cultivate in dark for 3 days, then place it under light for 10 hours every day, and cultivate for 30 days under the condition of 1500lx light intensity, count its bud induction rate and growth situation, and the ...

Embodiment 2

[0021] (1) Bud induction culture: take the stem section of Eucommia ulmoides No. 5 with buds as explants, cut off the leaves, cut into 3.0-5.0 long segments with 2-3 axillary buds and soak them in washing powder solution for 10 minutes , and then gently scrub the surface of the explant with a soft brush to remove the dust and some bacteria on the surface, then rinse it with tap water for 2 hours, then place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 15 seconds, and then wash it with sterile water for 5 seconds. sterilized with 0.1% mercuric chloride solution for 15 minutes, rinsed with sterile water for 4 times, and then dried the water droplets on the surface with sterile filter paper, and then inoculated into bud induction medium for bud induction. Cultivate in dark for 3 days, then place it under light for 10 hours every day, and cultivate for 30 days under the condition of 1500lx light intensity, count its bud induction rate and growth situation...

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PUM

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Abstract

The invention discloses a tissue culture method for Eucommia ulmoides Oliv., and relates to a method for quickly obtaining a large number of good-variety Eucommia ulmoides Oliv. nursery-grown plants within a short time through a plant tissue culture technology of Eucommia ulmoides Oliv.. According to the tissue culture method disclosed by the invention, stems with buds of the Eucommia ulmoides Oliv. are taken as explants, in-vitro regeneration of the Eucommia ulmoides Oliv. is realized through processes of bud induction, bud propagation culture, rooting culture, seedling hardening, transplanting, and the like, and technical support is provided for large-scale breeding, rapid propagation and new variety popularization.

Description

technical field [0001] The invention relates to a plant tissue culture method in plant biotechnology, in particular to a eucommia tissue culture method. Background technique [0002] Eucommia ( Eucommia ulmoides Oliv.) is a perennial deciduous tree of the genus Eucommia in the family Eucommia. It is a unique plant in my country and is mainly distributed in Yunnan, Sichuan, Guangxi and the middle and lower reaches of the Yangtze River. Eucommia ulmoides is a rare and valuable medicinal plant with various pharmacological effects, and it is also the most ideal Chinese medicinal material for human anti-aging and health care. Its dry bark can be used as medicine. It has the functions of nourishing the kidney, strengthening the waist, strengthening the tendons, strengthening the bones, anti-pregnancy, and lowering blood pressure. It is also known as the top grade of traditional Chinese medicine. In addition, Eucommia is also the raw material for extracting Eucommia gum, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨业云
Owner 杨业云