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Tissue culture rapid propagation technique of moringa oleifera Lam.

A technology of tissue culture rapid propagation and Moringa oleifera, which is applied in the field of plant tissue culture, can solve problems such as no reports, and achieve the effect of solving the problem of supplying high-quality Moringa oleifera seedlings

Inactive Publication Date: 2015-06-10
陈桂容
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, complete Moringa oleifera tissue culture rapid propagation technology has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Collection and processing of explants: Select Moringa oleifera oleifera plants with plump shoots of the year, soak in washing powder water for 10 minutes after removing leaves, rinse with tap water for 1 hour after brushing, and cut into 1-3cm long branches with axillary buds Stem sections were first sterilized with 75% alcohol for 5 seconds, then washed 3 times with sterile water, then sterilized with 0.1% mercuric chloride solution for 10 minutes, washed 5 times with sterile water, and then used for later use.

[0018] (2) Bud induction: inoculate the stem section with axillary buds obtained from the treatment in step (1) on the bud induction medium, culture it in total darkness at 28°C for 3 days, and then place it in the light for 10 hours a day, with a light intensity of 1500~2000lx cultured for 18 days to form clustered buds, and the induction rate reached 87.4%. The bud induction medium is: MS+6-BA 4mg / L+sucrose 25g / L+agar 4g / L, pH is 5.8.

[0019] (3) Bud p...

Embodiment 2

[0023] (1) Collection and processing of explants: Select Moringa oleifera oleifera plants with plump shoots of the year, soak in washing powder water for 15 minutes after removing leaves, rinse with tap water for 3 hours after brushing, and cut into 1-3cm long branches with axillary buds Stem segments were first sterilized with 75% alcohol for 10 seconds, then washed 3 times with sterile water, then sterilized with 0.1% mercuric chloride solution for 15 minutes, washed 5 times with sterile water, and then set aside.

[0024] (2) Bud induction: inoculate the stem section with axillary buds obtained from the treatment in step (1) on the bud induction medium, and culture it in total darkness at 28°C for 3 days, and then put it in the light for 11 hours a day, and the light intensity is 1500~2000lx cultured for 21 days to form clustered buds, and the induction rate reached 94.1%. The bud induction medium is: MS+6-BA7mg / L+sucrose 30g / L+agar 5g / L, pH is 5.8.

[0025] (3) Bud p...

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PUM

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Abstract

The invention discloses a tissue culture rapid propagation technique of moringa oleifera Lam. and relates to a seedling growing method for obtaining strain property stable moringa oleifera Lam. seedlings from the moringa oleifera Lam. by use of a vegetative rapid propagation technique. According to the method, the axillary buds of the moringa oleifera Lam. are taken as the explants, and the rapid propagation of the moringa oleifera Lam. seedlings is realized by virtue of the processes of bud induction, bud proliferation, rooting culture, acclimatization and transplanting and the like; the tissue culture rapid propagation technique has important practical significance on acceleration of the introduction and the artificial cultivation of the moringa oleifera Lam. and culturing of high-quality moringa oleifera Lam. seedlings.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a technique for tissue culture and rapid propagation of Moringa oleifera. Background technique [0002] Moringa ( Moringa oleifera Lam . ) is a plant of the genus Moringa in the Moringaceae family, which is a single genus plant in a single family. Moringa is native to the Himalayas in northern India. In 1960, Moringa was introduced from India as a greening tree to Southwest China. Currently, it is mainly cultivated in Fujian, Yunnan, Guangxi, Guangdong, Hainan and other places. Moringa is super rich in nutrients, including vitamins A, B, C, protein, calcium, iron, potassium and other mineral elements, so it is called a vegetable tree. Moringa at the seedling stage can be cooked as a whole. Fresh immature or mature fruit pods, immature germs, leaves, flowers, and roots are all edible, but at present Moringa consumers are mainly concentra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈桂容
Owner 陈桂容