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Composite microecological feed additive capable of increasing freshwater fish growth rate and application of composite microecological feed additive

A feed additive and compound micro-ecological technology, which is applied in the field of feed additives and compound micro-ecological feed additives, achieves the effect of high enzyme activity, rich enzyme system and reduction of anti-nutritional factors

Inactive Publication Date: 2015-06-10
QINGDAO GENYUAN BIOLOGICAL TECH GRP
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the situation that freshwater fish have poor digestibility of feed plant raw materials, the present invention provides a compound micro-ecological feed additive for improving the growth rate of freshwater fish and its application. The feed additive of the present invention can significantly improve the growth of freshwater fish Rate

Method used

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  • Composite microecological feed additive capable of increasing freshwater fish growth rate and application of composite microecological feed additive
  • Composite microecological feed additive capable of increasing freshwater fish growth rate and application of composite microecological feed additive
  • Composite microecological feed additive capable of increasing freshwater fish growth rate and application of composite microecological feed additive

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, the screening of dominant bacterial strain of the present invention

[0018] In this embodiment, 7 bacillus strains were selected for heat resistance and enzyme production performance comparison, wherein D-11, D-12, and D-14 were different substrains of Bacillus licheniformis, and K12 and K13 were Bacillus subtilis ( Different substrains of Bacillus subtilis) are commercial bacterial powders available on the market. Bacillus cereus is isolated from the bottom mud of the sea cucumber breeding area in Haiyangsuo Town, Rushan City, Weihai City, and Bacillus subtilis is a commercial bacterial powder.

[0019] First, use a high-temperature water bath to screen dominant strains, such as figure 1 As shown, after 20 minutes of water bath at 85°C, the survival rate was above 60%, among which Bacillus subtilis was the best, with a survival rate of 100%. Followed by strains D-12, D-11, D-14 were 95.23%, 93.88%, 89%. The survival rates of Bacillus subtilis, Bacillus...

Embodiment 2

[0026] The compound micro-ecological feed additive for improving the growth rate of freshwater fish of the present invention comprises the following components:

[0027]

[0028]

[0029] Among them, cellulase, xylanase, neutral protease and phytase are commercially available enzyme preparations, and commercially available bacterial powder is used for Bacillus subtilis, and the preservation number of Bacillus cereus is: CGMCC No.10052.

[0030] The Aspergillus niger solid fermentation compound enzyme is fermented by using bran and cotton meal to form a fermentation material, the mass ratio of the two is 7:3, and adding 4.0% (NH 4 ) 2 SO 4 , the ratio of material to water is 1:1.2, and the amount of bacteria used for the inoculation of Aspergillus niger is 2*10 9 seed solution per ml, the inoculum amount of the seed solution is 1L / ton of fermented material, the initial pH is 7.5, the culture temperature is 28°C, the culture time is 60h, and the material is turned twice ...

Embodiment 3

[0031] Embodiment 3, the influence of the present invention on the growth of carp fry

[0032] The experiment of this embodiment was carried out at the breeding base of Ocean University of China, Jimo National Oceanographic Research Center. The volume of the experimental aquarium was 45L, and the aquarium was continuously inflated during the breeding period. The experiment was divided into two groups, the experimental group added 5g / kg compound micro-ecological feed additive, and the control group did not add compound micro-ecological feed additive. Each group has 7 repetitions, one aquarium is one repetition, each aquarium is stocked with 8 carps, the size is 11.93g±0.52 / carp, the test period is 60d, and the weight is weighed once every 30d.

[0033] Table 2 Effects of compound microecological feed additives on the growth of carp fry

[0034]

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Abstract

The invention provides a composite microecological feed additive capable of increasing the freshwater fish growth rate and application of the composite microecological feed additive. The additive comprises the following components: aspergillus niger solid fermentation compound enzyme, bacillus subtilis, bacillus cereus, cellulose, xylanase, neutral protease and phytase. The composite microecological feed additive disclosed by the invention is added with the solid fermentation compound enzyme, so that the utilization rate of the freshwater fish to baits is effectively increased, the growth of fishes is promoted, the breeding of pathogenic bacteria including aeromonas hydrophila, vibrio alginolyticus and the like in a water body can be inhibited due to the addition of fungicides, the occurrence possibility of diseases of saprolegniasis, white skin diseases, bacillary enteritis and the like is reduced; compared with similar additives, the additive disclosed by the invention has the advantages of being safe, high in efficiency, special, free of residues, free of side effects and the like; the composite microecological feed additive can be used for improving and keeping the good growth state of the freshwater fish and effectively increasing the freshwater fish growth rate.

Description

technical field [0001] The invention belongs to the technical field of aquatic feed and relates to a feed additive, in particular to a compound micro-ecological feed additive for improving the growth rate of freshwater fish and an application thereof. Background technique [0002] The increasing shortage of animal raw materials has brought major challenges to the development of aquatic feed. At present, plant-based raw materials are being widely used in aquatic feed. However, freshwater fish have poor ability to digest and utilize plant raw materials. For example, freshwater fish lack phytase and cannot fully utilize phytate phosphorus. digestion and absorption. Studies have shown that cellulase, xylanase, phytase and other compound enzymes can be used for enzymolysis to stimulate the secretion of endogenous digestive enzymes, improve the digestion and absorption rate of plant protein and starch, and promote the growth of aquatic animals. Aspergillus niger solid fermentat...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/16A23K1/165A23K1/00
Inventor 谢金萍梁晶晶曲朋张明俊
Owner QINGDAO GENYUAN BIOLOGICAL TECH GRP
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