Method for observing DRG and spinal cord structure of animal, and recording spinal cord electrical activities of animal

A spinal cord and animal technology, applied in the field of animal DRG and spinal cord research, can solve the problems of inability to distinguish between motor and sensory axons, and inability to observe, achieve good braking effect, reduce pain and pressure, and promote healing or regression. changing effect

Inactive Publication Date: 2015-06-10
张金辉 +4
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  • Abstract
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Problems solved by technology

In the study of ganglia, it is still in the stage of in vitro experiments; in the study of spinal cord axons, it is limited to the observation of the dorsal side under anesthesia, and cannot be observed in the awake state. motor and sensory axons could not be differentiated or even controlled in the state

Method used

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Embodiment 1

[0032] The method for observing animal DRG, spinal cord structure and recording spinal cord electrical activity of the present invention comprises the following steps:

[0033] S100, YFP / GFP mouse DRG surgery

[0034] S101. Select adult YFP or GFP mice (YFP and GFP mice are transgenic fluorescent mice, and fluorescence observation can also be realized by fluorescent agent injection for ordinary animals) and inject 0.08 mg of 10 mg / mL pentobarbit in its abdominal cavity Sodium;

[0035] S102. After anesthesia, transfer the mouse to a place far away from the operation area for shaving of the corresponding part, and clean it with 70% alcohol, then apply an appropriate amount of depilatory cream to the corresponding shaving area, wait for 3 minutes to 5 minutes after applying it evenly, and then use it. Use the scraper to scrape off the depilatory cream and rat hair in the same direction; use a cotton ball dipped in normal saline to gently wipe off the residual depilatory agent a...

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Abstract

The invention relates to a method for observing the DRG and spinal cord structure of an animal, and recording spinal cord electrical activities of the animal. The method comprises the following steps: (1) a DRG operation; (2) a DRG virus injection; (3) an operation for simultaneous observing the spinal cord side axon and the DRG; (4) an operation for observing the dorsal axon; (5) a spinal operation for inserting potential difference monitoring electrodes; (6) imaging. The method is used for observing the comparison between the dorsal axon and the ventral axon of the animal as well as the spinal cord electrical activities, so that repeated operations during the imaging can be avoided, the pain and pressure suffered by a tested animal can be relieved, the spinal cord side of the animal can be observed, a comparative research on the motor neuron axon and the sensory neuron axon of the animal can be achieved, and the spinal cord dorsal side of the animal can be observed when the animal is sober; through the adoption of the method, all-directional connection of the whole nerve system can be achieved, researches on the structure and function operating mechanism of the whole nerve system can be promoted, and a feasible method for integrated researches and analysis of sleeping, learning and other animal behaviors can be provided.

Description

technical field [0001] The invention relates to the technical field of animal DRG and spinal cord research, in particular to a method for observing the animal DRG spinal cord structure and recording the electrical activity of the spinal cord. Background technique [0002] Because of the lack of cell-level in vivo imaging methods, and because of the lack of immobilization imaging methods in the awake state of the living body, our research on the dorsal root ganglia is still in the state of in vitro research, and we cannot observe the side of the spinal cord and the axis of motor and sensory neurons. In the control study, the dorsal side of the spinal cord cannot be observed in an awake state, and the spinal cord insertion electrode is easy to fall off, which needs to be solved. [0003] It is of unique value in neurophysiological and pathological research to observe the structure and function of animal cortex at a depth of 1mm under a nonlinear microscope in vivo or even in a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/00A61B5/04A61D1/00A61D7/00
CPCA61B5/004A61B5/0071A61D1/00A61D7/00A61B5/24
Inventor 张金辉
Owner 张金辉
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