Saffron endophytic fungus, crude polysaccharide and its preparation method and application

A technology of endophytic fungi and saffron, applied in biochemical equipment and methods, methods based on microorganisms, fungi, etc., to achieve the effect of inhibiting red blood cell hemolysis, scavenging free radical lipid peroxidation, and obvious dose-effect correlation

Active Publication Date: 2018-02-13
温露
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there are very few research reports on the endophytic fungi of saffron, and the development and application of the crude polysaccharides of the endophytic fungi of saffron need further exploration and research

Method used

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  • Saffron endophytic fungus, crude polysaccharide and its preparation method and application
  • Saffron endophytic fungus, crude polysaccharide and its preparation method and application
  • Saffron endophytic fungus, crude polysaccharide and its preparation method and application

Examples

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Effect test

Embodiment 1

[0040] This example is an example of the preparation method of the crude polysaccharide of the endophytic fungus of saffron.

[0041] 1. Preparation of medium:

[0042] PDA culture medium and PYG liquid culture medium described in the present invention are prepared by the following method.

[0043] (1) PDA medium (potato dextrose agar): 200g potatoes (peeled, cut into small pieces, boiled in appropriate deionized water for 20min, filtered through gauze, discarded mashed potatoes, and took the filtrate), 10g glucose, 15g agar, natural pH, Dilute to 1000mL with deionized water.

[0044] (2) PYG liquid medium: yeast extract 1g, peptone 2g, glucose 10g, sodium chloride 2g, pH 6.8-7.2, deionized water to 1000mL.

[0045] Before use, the culture medium was sterilized at a temperature of 121°C and a pressure of 0.1 MPa for 20 minutes.

[0046] 2. Activation of strains:

[0047]The endophytic fungus is isolated from healthy saffron bulbs through a strict surface disinfection metho...

Embodiment 2

[0059] This embodiment is a series of in vitro antioxidant tests carried out on the saffron endophytic fungus crude polysaccharide prepared in Example 1, and the obtained test results are as follows Figure 4 ~ Figure 8 shown.

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Abstract

The present invention provides a saffron endophytic fungus (Penicillium sp.) with a preservation number of CCTCC No. M2013472, and also provides a crude polysaccharide prepared by the saffron endophytic fungus, a preparation method and application thereof. The prepared saffron endophytic fungus crude polysaccharide has good antioxidant activity and can be used as a natural antioxidant in the fields of food, medicine, cosmetics and the like.

Description

technical field [0001] The invention relates to a biological polysaccharide and its preparation method and application, in particular to a saffron endophytic fungus, saffron endophytic fungus crude polysaccharide, its preparation method and application. Background technique [0002] Saffron (Crocussativus L.), also known as saffron and saffron, is a perennial herbaceous plant of the genus Crocus (Crocus L.) in the family Iridaceae. It is a kind of precious traditional Chinese medicinal material in our country. Its nature and taste are sweet and flat, and it has the effects of promoting blood circulation and removing blood stasis, relieving stagnation and calming nerves, enhancing immunity and lowering cholesterol. In recent years, with the rapid development of modern medicine, pharmacy and molecular biology technology, it has been found that saffron has good effects in anti-tumor, nervous system protection and anti-cardiovascular system diseases, with almost no toxic side e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/04A23L33/125A61K31/715A61K8/73A61P39/06C12R1/80
Inventor 温露陈钢韦啟球
Owner 温露
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