Optical fiber microsensor and detection system for in situ determination of biofilm density distribution

Abstract

The invention discloses an optical fiber microsensor and detection system for in-situ measurement of biofilm density distribution. The optical fiber microsensor includes a glass sleeve, a metal ferrule and a single-mode optical fiber; the optical fiber resin coating at the front end of the single-mode optical fiber has been peeled off, exposing a glass core tip about 1mm long. There is a metal ferrule 1cm above the tip of the optical fiber, and the single-mode optical fiber is fixed in the glass sleeve through the metal ferrule. A detection system for in-situ measurement of biofilm density distribution includes a light source, a transmission optical fiber, an optical fiber coupler, an incident signal detector and a backscattered signal detector, a signal amplifier, an optical fiber microsensor and a computer. Compared with traditional methods, the optical fiber microsensor of the present invention has the advantages of high resolution, short response time, and high sensitivity. And it does not require precise chemical analysis, so the measurement results are accurate, reproducible, and the system error is very small. More importantly, it enables in-situ online monitoring without damaging the biofilm structure.

Description

Technical field [0001] The invention relates to an optical fiber microsensor, in particular to an optical fiber microsensor and detection system for in-situ measurement of biofilm density distribution. technical background [0002] Biofilm plays a very important role in water environment restoration and water treatment and purification systems. Depending on the type of microorganisms, external shear force, matrix composition and age, biofilms have complex structures that vary greatly, which will affect the mass transfer and diffusion mechanisms of biofilms. Biofilm density is the most basic data that characterizes its structural differences and is also the most direct factor affecting mass transfer. The means of measuring biofilm density are very limited. It is generally obtained by measuring the dry weight of a known volume of biofilm and then calculating it. The testing process will destroy the biofilm and the result error will be large. More importantly, traditional met...

AI Technical Summary

Problems solved by technology

More importantly, traditional methods can only obtain the average density of the entire biofilm, which cannot accurately reflect the complex changes in its internal structure, let alone online continuous analysis of living biofilms.

Images