Optical fiber microsensor and detection system for in situ determination of biofilm density distribution

Abstract

The invention discloses an optical fiber micro-sensor and a detection system for measuring the density distribution of biological membrane in situ. The optical fiber micro-sensor includes a glass sleeve, a metal ferrule and a single-mode optical fiber; the optical fiber resin coating at the front end of the single-mode optical fiber has been stripped off, exposing a glass core tip with a length of about 1 mm. A metal ferrule is sheathed 1 cm above the tip of the optical fiber, and the single-mode optical fiber is fixed in the glass sleeve through the metal ferrule. A detection system for in-situ determination of biofilm density distribution includes a light source, a transmission optical fiber, an optical fiber coupler, an incident signal detector and a backscattered signal detector, a signal amplifier, an optical fiber microsensor and a computer. Compared with the traditional method, the present invention has the advantages of high resolution, short response time and high sensitivity. And precise chemical analysis is not required, so the measurement results are accurate, repeatability is good, and the system error is very small. More importantly, it enables in situ online monitoring without destroying the biofilm structure.

Description

technical field [0001] The invention relates to an optical fiber microsensor, in particular to an optical fiber microsensor and a detection system for measuring biofilm density distribution in situ. technical background [0002] Biofilm plays a very important role in water environment restoration and water treatment and purification systems. According to the different types of microorganisms, external shear force, matrix composition and age, biofilm has a complex structure with large differences, which will affect the mass transfer and diffusion mechanism of biofilm. Biofilm density is the most basic data to characterize its structural differences, and it is also the most direct factor affecting mass transfer. The means of measuring biofilm density are very limited. Generally, it is obtained by measuring the dry weight of a known volume of biofilm and then calculating it. The test process will destroy the biofilm, and the result error is large. More importantly, traditiona...

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Problems solved by technology

More importantly, traditional methods can only obtain the average density of the entire biofilm, which cannot accurately reflect the complex changes in its internal structure, let alone online continuous analysis of living biofilms.

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