Immune complex fluorescence detection method based on image self-adaption division

An immune complex and fluorescence detection technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of inaccuracy, background reflection interference, inconsistency, etc., to improve accuracy and repeatability, and eliminate the randomness of morphology and inconsistency, the effect of increasing speed

Inactive Publication Date: 2015-06-24
SHENZHEN KINGFOCUS BIOMDICAL ENG CO LTD
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Problems solved by technology

[0005] The purpose of the present invention is to provide a fluorescent detection method for immune complexes based on image adaptive segmentation, which solves the randomness, inconsistency, and interference caused by background reflections in complex background fluorescent complexes, and solves the problems caused by this. Photoelectric detection speed is slow, inaccurate technical problems

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  • Immune complex fluorescence detection method based on image self-adaption division
  • Immune complex fluorescence detection method based on image self-adaption division
  • Immune complex fluorescence detection method based on image self-adaption division

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Embodiment Construction

[0022] The present invention will be further elaborated below with reference to the accompanying drawings.

[0023] see figure 1 , which is a flowchart of an immune complex fluorescence detection method based on image adaptive segmentation of the present invention.

[0024] The first step S1 is to use the median filter for the fluorescent immune image, that is, to replace the value of the pixel with the gray value of the adjacent pixels of the pixel, and the output of the two-dimensional median filter is g(x,y)=med{ f(x-k,y-l), (k,l∈W)}.

[0025] Among them: f(x, y), g(x, y) are the original image and the processed image respectively. Set of coordinates for a rectangular subimage window of size m×n.

[0026] In the second step S2, the segmentation threshold is estimated according to the luminescence intensity curve of the immunofluorescence area, and then the image is binarized. The luminous intensity distribution curve of the fluorescent area is attached figure 2 shown. ...

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Abstract

The invention provides an immune complex fluorescence detection method based on image self-adaption division. The immune complex fluorescence detection method comprises the following steps: step 1, pre-processing an image, namely carrying out median filtering processing on a fluorescence immune image, reducing noise point interferences and keeping the edge steep; step 2, carrying out image binaryzation, namely estimating a division threshold value according to an immune fluorescence region light intensity curve and then carrying out the binaryzation on the image; step 3, positioning a target position, namely positioning the target position based on target positioning of narrow strip characteristics; step 4, calculating a target gray value, namely after the position of the target region is obtained, calculating a gray value and a background gray value of the target region; and step 5, outputting the gray value to obtain the content of a detected object. According to the light detection method provided by the invention, the randomness and the inconsistency of shapes of an immune fluorescence composite distribution region can be eliminated and the interferences caused by background region reflection are eliminated, and the precision and the repeatability of immune complex fluorescence detection are improved; and meanwhile, the detection processing speed is also increased.

Description

technical field [0001] The invention relates to the technical field of image detection, in particular to an immune complex fluorescence detection method based on image adaptive segmentation. Background technique [0002] Immunofluorescence is one of the earliest developed labeling immunotechniques. For a long time, some scholars have attempted to combine antibody molecules with some tracer substances, and use antigen-antibody reactions to locate antigenic substances in tissues or cells. Coons was equal to the first successful labeling with fluorescein in 1941. [0003] Immunofluorescence technology is to label the antibody with a fluorochrome that does not affect the activity of the antigen and antibody, bind to the corresponding antigen, and go through the chromatography process, respectively solidify in the detection area and the quality control area, and present a specific color under the fluorescence microscope. Sexual fluorescence response. Use fluorescently labeled ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 汪国有龚志文张二盈章国建
Owner SHENZHEN KINGFOCUS BIOMDICAL ENG CO LTD
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