Salmonella vaccine for cross protection

A technology of Salmonella and Salmonella enterica, applied in vaccines, veterinary vaccines, bacteria, etc., can solve the problem of not inhibiting the reactivity of monoclonal antibodies

Active Publication Date: 2018-11-06
INTERVET INT BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, reactivity with the monoclonal antibody was inhibited by preincubation of the corresponding antigen from serogroup C2-3 with polyclonal rabbit anti-serogroup C2-C3 antibody, but reactivity with this monoclonal antibody was inhibited by incubation of the antigen with antigen from serogroup C1. , or any other Salmonella serogroup antigen tested did not inhibit the reactivity of this monoclonal antibody [Duffey et al. J. Clin. Microbiol. 30(12):3050-3057(1992)]

Method used

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  • Salmonella vaccine for cross protection
  • Salmonella vaccine for cross protection
  • Salmonella vaccine for cross protection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Comparison of vaccines containing different Salmonella serotypes

[0078] Preparations containing Salmonella hadar alone or with inactivated ( S. Hadar; SH) and / or Salmonella infantis ( S. Infantis; SI) serotype combined, inactivated Salmonella enteritidis ( S. Enteritidis; SE) and Salmonella typhimurium ( S. Typhimurium; ST) (NB: S. Enteritidis and S. Typhimurium serotypes are the same as those in SALENVAC T) serotypes. All serotypes were grown in iron-limited media and formalin inactivated prior to use. The vaccine formulations used in the study are listed in Table 1.

[0079] Table 1: Vaccine Compositions

[0080]

[0081] SE / ST / SH / SI Quadrivalent Vaccine 1 (Quad 1) contains an additional amount of adjuvant, as previous studies with similar vaccines containing high cell numbers have shown that 25% aluminum hydroxide content is 9 cells / ml of vaccine may not be sufficient. SE / ST / SH / SI Quad 2 (Quad 2) contained 25% adjuvant to investigate adjuvant conce...

Embodiment 2

[0118] Passive protection in broiler chickens challenged with S. hadar or S. infantis at 4 days of age

[0119] Parental birds were vaccinated with the S. enteritidis + S. typhimurium + S. infantis (SE / ST / SI) combination vaccine with 25% Rehydrogel™, or were optionally not vaccinated as a control. Four-day-old broiler chickens that had hatched from eggs of vaccinated or non-vaccinated hens were challenged with S. hadar or S. infantis to test passive protection against these two different serotypes.

[0120] Recovery of challenge serotypes from study birds was high. No differences were seen between vaccinated and control groups with respect to challenge shedding, monitored by cloacal swabs or in cecal contents post mortem. However, when invasion of both liver and spleen was considered, fewer positive birds were present in the vaccinated group than in the control group. It was found that this was sampled 10 days after the attack and using 10 2 CFU Hadar Salmonella or 10 3...

Embodiment 3

[0139] Efficacy test of a trivalent vaccine against Salmonella hadar challenge in laying hens at 14 weeks of age

[0140] summarize

[0141] Six-week-old SPF origin laying chickens were immunized with a trivalent vaccine containing equal numbers of formalin-killed cells of S. grow under restricted conditions. The second vaccine dose was given at 10 weeks of age. Four weeks after the second vaccination, birds and unvaccinated cohorts were challenged with S. Hadar by the oral route, and shedding of the challenge strain was monitored by cloacal swab examination. Dissemination of challenge bacteria to guts was determined by postmortem examination of the birds at 10 and 14 days post-challenge.

[0142] When individual bird shedding levels were compared to controls, significantly fewer challenge organisms were shed by the vaccinated group (p=0.001).

[0143] The proportion of positive spleen samples from control birds was significantly higher than in vaccinated groups (p = 0.0...

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Abstract

The present invention discloses that Salmonella enterica serogroup C2-3 serotype cross-protects against Salmonella enterica serogroup C1 serotype and vice versa. Therefore, the present invention discloses the use of Salmonella enterica serogroup C2-3 serotype or Salmonella enterica serogroup C1 serotype for the preparation of a vaccine for administration to poultry to protect against diseases caused by Salmonella enterica serogroups Conditions of serotype C2‑3 and / or conditions resulting from Salmonella enterica serogroup C1 serotype.

Description

field of invention [0001] The present invention relates to Salmonella enterica ( Salmonella enterica ) serogroups C2-3 serotypes with Salmonella enterica serogroup C1 serotypes allows the preparation of vaccines containing serotypes from only one of these two different serogroups to protect against any A Salmonella enterica serogroup. Background technique [0002] salmonella( Salmonella ) is a major pathogen of poultry and its presence in poultry populations is a major public health concern for humans. Food poisoning incidents caused by Salmonella remain prevalent worldwide and are second only to Campylobacter ( Campylobacter ) of the most common causes of food poisoning due to bacteria. Not surprisingly, poultry products are a major source of Salmonella infection in humans, and this pathogen originates from the transfer of laying hens to eggs, or from broiler carcasses to meat products. Salmonella enteritidis (SE) is of particular importance in egg-related food poiso...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/02A61K39/112
CPCA61K39/0275A61K2039/522A61K2039/552A61P31/04Y02A50/30A61K39/02C12N1/20A61K39/39A61K2039/55505
Inventor C.普格
Owner INTERVET INT BV
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