A kind of production method of soft acid flavoring agent
A production method and flavoring agent technology, applied in the field of soft acidic flavoring agent production, can solve the problems of long fermentation cycle, miscellaneous flavors, difficulty in meeting market demand, etc., achieve mature production process and improve aroma coordination , the effect of convenient storage
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Embodiment 1
[0041] Embodiment 1: produce acid flavoring according to the following method
[0042] (1) Yeast strain cultivation: select 2 rings of cerevisiae yeast strain slant, inoculate it on the wort medium, cultivate it on a shaker at 28°C and 120r / min for 30h, and the number of bacteria is 10 6 more than 1 / mL, to obtain the first-grade seed liquid; adjust the pH of the wort culture liquid to 4.5, use a fermenter at 28°C, aerobic fermentation for 24 hours, and expand the culture to 10 6 ~10 7 Individual / mL or more, to obtain the secondary seed liquid of yeast;
[0043] (2) Cultivation of hexanoic acid functional strains: select 2 slants of cyclohexanoic acid functional strains, culture them on sodium acetate medium at 35°C for 5 days, and the number of bacteria is 10 7 More than / mL, the first-grade seed liquid is obtained; add 0.5% of its mass rice flour to the sodium acetate medium, adjust the pH value of the fermentation liquid to 6.8, press 20% of the inoculation amount, cultiva...
Embodiment 2
[0048] Embodiment 2: produce acid flavoring according to the following method
[0049] (1) Yeast strain cultivation: select 2 rings of cerevisiae yeast strain slant, inoculate it on the wort medium, cultivate it at 30°C, 100r / min shaker for 24h, and the number of bacteria is 10 6 more than 1 / mL, get the first-class seed solution; adjust the pH of the wort culture solution to 4.8, use a fermenter at 32°C, aerobic fermentation for 30 hours, and expand the culture to 10 6 ~10 7 Individual / mL or more, to obtain the secondary seed liquid of yeast;
[0050] (2) Cultivation of hexanoic acid functional strains: select 2 slants of cyclohexanoic acid functional strains, culture them on sodium acetate medium at 37°C for 4 days, and the number of bacteria is 10 7 More than / mL, the first-grade seed liquid is obtained; add 0.3% of its mass rice flour to the sodium acetate medium, adjust the pH value of the fermentation liquid to 7.0, and cultivate it at 37°C for 7 days according to 15% i...
Embodiment 3
[0055] Embodiment 3: produce acid flavoring according to the following method
[0056] (1) Yeast strain cultivation: select 2 rings of cerevisiae yeast strain slant, inoculate it on the wort medium, cultivate it on a shaker at 32°C and 150r / min for 28h, and the number of bacteria is 10 6 more than one per mL, to obtain the first-grade seed liquid; adjust the pH of the wort culture liquid to 5, and use a fermenter at 30°C for 26 hours of aerobic fermentation to obtain the second-grade seed liquid of yeast according to the inoculation amount of 8%.
[0057] (2) Cultivation of hexanoic acid functional strains: select 2 slants of cyclohexanoic acid functional strains, culture them on sodium acetate medium at a temperature of 37°C for 5 days, check under a microscope, and the number of bacteria per milliliter is maintained at 10 7 above, to obtain a first-grade seed liquid; add 0.5% of its mass rice flour to the sodium acetate medium, adjust the pH value of the fermentation liquid ...
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