Heavy metal strongly inducible promoter cd‑s4 and its application

A technology of heavy metals and promoters, applied in the fields of application, recombinant DNA technology, angiosperms/flowering plants, etc., can solve the problems of limited gene resources and few promoters, reduce environmental hazards, improve utilization rate, and improve phytoremediation effect of effect

Active Publication Date: 2017-10-13
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are not many promoters specifically induced by heavy metals such as cadmium and nickel, so when people want to study the interaction between plants and heavy metals and the research on heavy metal governance, the gene resources that can be selected are limited.

Method used

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  • Heavy metal strongly inducible promoter cd‑s4 and its application
  • Heavy metal strongly inducible promoter cd‑s4 and its application
  • Heavy metal strongly inducible promoter cd‑s4 and its application

Examples

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Embodiment Construction

[0040] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0041] The present invention utilizes mature Nipponbare seeds (preserved by the Biotechnology Laboratory of the Rice Institute of Anhui Academy of Agricultural Sciences) to isolate promoter fragments.

[0042] The following is a brief description of the main medicinal raw materials and reagent materials used in the examples. Unless otherwise specified, the raw materials and reagents are all commercially available products.

[0043] Sodium hypochlorite (NaClO, available chlorine concentration 4%), Tween20 was purchased from Sigma Company. Hygromycin B was purchased from Roche; PEASY-Tsimple and DNA marker-Trans2K were purchased from Transgen; restriction enzymes were purchased from NEB; KOD high-fidelity polymerase ...

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Abstract

The present invention provides a strong induction expression promoter of heavy metals such as cadmium and nickel, and the promoter can regulate the concentrated expression of target genes under the induction condition of heavy metals such as cadmium and nickel. The strong inducible expression promoter of heavy metals such as cadmium and nickel of the present invention comprises: the nucleotide sequence as described in SEQ ID No: 1 or 2 of the sequence listing or derivatives thereof. The invention also provides a recombinant expression vector, an expression cassette constructed by using the promoter and its application in plant cultivation. By linking the promoter of the present invention to the gene to be expressed and using the vector to transfer it into the plant, it can drive the expression of the foreign gene in various parts of the plant. With the development of molecular biology and developmental biology, people have an increasingly strong demand for the specific inducible expression promoter, and the promoter provided by the present invention just meets such demand. In particular, it has very huge potential commercial value for the cultivation and breeding of heavy metal-resistant plants.

Description

technical field [0001] The present invention relates to a promoter isolated from plants, in particular to heavy metals such as cadmium and nickel strongly inducible expression promoters isolated from rice, the present invention also relates to recombinant expression vectors containing the heavy metals such as cadmium and nickel strongly inducible expression promoters and The application in improving plant traits, cultivating new plant varieties, etc. belongs to the field of separation and application of plant tissue or organ-specific expression promoters. Background technique [0002] Soil not only provides support for the growth of plants, but also provides fertility elements such as water, fertilizer, and air to plants to promote their growth and development. In recent years, due to the astonishing growth rate of population, the rapid development of industry, and the excessive use of agricultural chemical fertilizers and pesticides, the area of ​​soil pollution has rapidly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/82C12N5/10A01H5/00
Inventor 李娟杨剑波秦瑞英邱春红李莉魏鹏程李浩张银萍
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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