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Stable 5 minute - ribonucleotide hydrolytic enzyme detection kit

A ribonucleotide and detection kit technology, which is applied in the field of 5'-ribonucleotide hydrolase detection kits, can solve problems such as waste, influence on reagent stability, unfavorable long-term storage of reagents, etc., so as to achieve enhanced stability. Effect

Inactive Publication Date: 2015-07-01
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Due to the existence of various enzymes in the 5'-ribonucleotide hydrolase detection kit (colorimetric method), the stability of the reagent will be affected, which is not conducive to the long-term storage of the reagent, resulting in adverse consequences of waste

Method used

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  • Stable 5 minute - ribonucleotide hydrolytic enzyme detection kit
  • Stable 5 minute - ribonucleotide hydrolytic enzyme detection kit
  • Stable 5 minute - ribonucleotide hydrolytic enzyme detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Described in this implementation case A conventional 5'-ribonucleotide hydrolase detection kit, which comprises reagent components is composed of reagent R1 and reagent R2, the component content is as follows:

[0038] Example 1:

[0039] Reagent R1 components:

[0040] Goods buffer (PH7.6) 100mmol / L

[0041] 4-AA 2.3mmol / L

[0042] PNP 0.2U / mL

[0043] XO 0.3U / mL

[0044] POD 0.5U / mL

[0045] Reagent R2 components:

[0046] Goods buffer (PH7.6) 100mmol / L

[0047] 5'-Inosine nucleotide 13mmol / L

[0048] EHSPT 4mmol / L

[0049] The method of using a conventional 5'-ribonucleotide hydrolase detection kit (colorimetric method) described in this example is to use an automatic biochemical analyzer with dual reagent functions, such as Toshiba 120 automatic analyzer, and the operation is as shown in the table 1.

[0050] Table 1 Method of using a 5'-ribonucleotide phosphohydrolase detection kit

[0051]

Embodiment 2

[0053] Described in this implementation case A stable 5'-ribonucleotide hydrolase detection kit, which includes reagent components consisting of reagent R1 and reagent R2, the components are as follows:

[0054] Reagent R1 components:

[0055] Goods buffer (PH7.6) 100mmol / L

[0056] 4-AA 2.3mmol / L

[0057] PNP 0.2U / mL

[0058] XOD 0.3U / mL

[0059] POD 0.5U / mL

[0060] 4-FPBA 0.01%

[0061] NaCl 5mmol / L

[0062] Mannitol 5g / L

[0063] Triton 100 1%

[0064] BSA 0.1g / L

[0065] Reagent R2 components:

[0066] Goods buffer (PH7.6) 100mmol / L

[0067] 5'-Inosine nucleotide 13mmol / L

[0068] EHSPT 4mmol / L.

[0069] The method of using a stable 5'-ribonucleotide phosphohydrolase detection kit (colorimetric method) in this example is the same as that in Example 1.

Embodiment 3

[0071] Described in this implementation case A stable 5'-ribonucleotide hydrolase detection kit, which includes reagent components consisting of reagent R1 and reagent R2, the components are as follows:

[0072] Reagent R1 components:

[0073] Goods buffer (PH7.6) 100mmol / L

[0074] 4-AA 2.3mmol / L

[0075] PNP 0.2U / mL

[0076] XOD 0.3U / mL

[0077] POD 0.5U / mL

[0078] 4-FPBA 0.01%

[0079] NaCl 5mmol / L

[0080] Mannitol 5g / L

[0081] Triton 100 1%

[0082] BSA 0.5g / L

[0083] Reagent R2 components:

[0084] Goods buffer (PH7.6) 100mmol / L

[0085] 5'-Inosine nucleotide 13mmol / L

[0086] EHSPT 4mmol / L.

[0087] The method of using a stable 5'-ribonucleotide phosphohydrolase detection kit (colorimetric method) in this example is the same as that in Example 1.

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Abstract

The invention discloses a 5minute - ribonucleotide hydrolytic enzyme colorimetric method detection kit, which belongs to the technical field of clinic extracorporeal detection reagents. The kit comprises a reagent R1 and a reagent R2. A compound stabilizer formed by five components of 4-FPBA, NaCl, mannitol, Triton100 and BSA is added in the reagent component 1, so that the difficult problem that enzyme cannot be stored stably for a long time is solved, the stabilization time of the enzyme can be increased during the test, the enzyme activity cannot be influenced, the stability of the kit is effectively enhanced, the accuracy of the reagent and the sensitivity for analysis cannot be influenced, and the reagent can be further favorably popularized in market.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a 5'-ribonucleotide hydrolase detection kit. Background technique [0002] The full name of 5'-nucleotidase is 5'-ribonucleotide phosphohydrolase (5'-Nucleoticlase, 5'-NU or 5'-NT), which is widely distributed in liver, gallbladder, pancreas, intestine, heart, brain, In organs and tissues such as the lung, kidney, pituitary, thyroid, prostate, and testis, it is located on the cell membrane, and in the liver, it mainly exists in the bile duct and sinusoidal space. [0003] The reference value range of 5'-ribonucleotide phosphohydrolase in normal human serum is 0-10U / L, which plays an important catalytic role in the catabolism of 5'-inosine nucleotides and can catalyze 5' - Inosine nucleotides are degraded into inosine nucleosides, which are then catalyzed by nucleotide phosphorylases to generate hypoxanthine, and finally oxidized to the metabolite uri...

Claims

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Application Information

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IPC IPC(8): C12Q1/42C12Q1/28C12Q1/26
Inventor 甘宜梧董雯谭柏清王琦王春艳肖慧
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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