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Microorganism bacterium agent for sludge reduction onsite as well as preparation method and application of microorganism bacterium agent

A technology of sludge in-situ reduction and microbial agent, which is applied in the field of environmental protection, can solve the problems of reducing sludge production, achieve low cost, improve effect, and simple operation

Active Publication Date: 2015-07-22
ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Microorganisms aggregate together through extracellular polymer (EPS) to form a large amount of sludge. In order to solve the problem of a large amount of sludge treatment, the present invention provides a microbial agent for in-situ reduction of sludge and its preparation method and Application, using the biolysis method to degrade the macromolecular refractory substances in the sludge, adding the microbial agent of the present invention can significantly reduce the output of the sludge

Method used

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  • Microorganism bacterium agent for sludge reduction onsite as well as preparation method and application of microorganism bacterium agent
  • Microorganism bacterium agent for sludge reduction onsite as well as preparation method and application of microorganism bacterium agent
  • Microorganism bacterium agent for sludge reduction onsite as well as preparation method and application of microorganism bacterium agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Preparation of Bacillus amyloliquefaciens powder: slant seeds-shake flask seeds-seed tank-fermenter tank-freeze-drying.

[0045] (1) Slope seeds: peptone 10g / L, beef extract 5g / L, NaCl 5g / L, 2% agar, pH 7.0. Sterilize at 121°C for 20min. Streak inoculation on the culture medium and culture at 35°C for 24h.

[0046] (2) Shake flask seeds: peptone 10g / L, beef extract 5g / L, NaCl 5g / L, pH 7.0. Sterilize at 121°C for 20min. Pick a small amount of bacteria from the activated slant seeds and inoculate them in the liquid seed culture medium, culture at 180 rpm, 35° C. for 20 h.

[0047] (3) Seed tank: culture medium components peptone 10g / L, beef extract 5g / L, NaCl 5g / L, pH 7.0, add 30L seed medium into 50L seed tank, sterilize with high pressure and damp heat at 121°C, cool to 33°C 10% (V / V) inoculum was inoculated into the seed tank by the shake flask bacterial classification, cultivated to the logarithmic growth phase at 35°C, the stirring speed was 180 rpm, and the ste...

Embodiment 2

[0062] The laboratory uses a 1L beaker, filled with 1L of sludge, and the sludge concentration SV 30 28%, set a blank sample without bacterial agent, a control group heated and stirred without bacterial agent, and an experimental group controlled by a magnetic heating stirrer, control the speed at 150rpm, 30°C, and add 0.1‰ of bacterial agent. Table 1 shows the results of the small test after 2, 4, and 6 days of treatment.

[0063] Small-scale test of table 1 microbial agent on sludge reduction

[0064]

[0065] From the test results, after 2, 4, and 6 days of treatment with bacterial agents, the treatment efficiency is not high but tends to decline in a short time. The treatment effect of Bacillus amyloliquefaciens and S.

Embodiment 3

[0067] In an urban sewage treatment plant, the in-situ sludge reduction experiment was carried out by adding bacterial agents. The sewage treatment plant has a daily water treatment capacity of 5,000 tons. There are two parallel processes, both of which are anoxic / anaerobic / aerobic (A2O) processes. One group is used as the control group, and the other group is used as the experimental group. Bacteria are added to the aerobic pool of the experimental group. First add 5 times of tap water to the microbial agent prepared in Example 1 to aerate and activate it for 24 hours, then add it to the water inlet of the aerobic pool with a peristaltic pump, and the flow rate is 0.1‰ of the treated water. When the sludge properties of the experimental group are significantly improved , the amount of sludge produced began to decrease, and at this time, the flow rate of the bacterial agent was changed to 0.01‰. During the process of adding the bacterial agent, the sludge concentration in the ...

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Abstract

The invention discloses a microorganism bacterium agent for sludge reduction onsite as well as a preparation method and application of the microorganism bacterium agent. The microorganism bacterium agent is prepared from bacillus amyloliquefaciens CGMCC No 1.769 and saccharomyces cerevisiae CGMCC No 2.3853. Fermentation liquor of the bacillus amyloliquefaciens and fermentation liquor of the saccharomyces cerevisiaeare respectively frozen and dried into powder; the two types of powder are mixed to form the microorganism bacterium agent. The microorganism preparation disclosed by the invention is used for domestic sludge reduction onsite and the microorganism bacterium agent is added into an aerobic tank of a municipal sewage treatment plant. The addition quantity of the microorganism bacterium agent is 0.01 permillage to 0.1 permillage of the quantity of processed water in the aerobic tank. According to the invention, a biological cell lysis method is utilized to degrade macro molecular hard-degraded substances in sludge and addition of the microorganism bacterium agent can obviously reduce yield of the sludge. Compared with the prior art, the microorganism bacterium agent has the characteristics of high efficiency, low cost and high simplicity for operation and is suitable for sludge reduction onsite of all municipal sewage treatment plants.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a microbial agent for in-situ reduction of sludge and its preparation method and application. Background technique [0002] With the great improvement of my country's urban sewage treatment capacity, the amount of excess sludge generated in the corresponding sewage treatment process is also increasing. Compared with sewage, sludge is more toxic. At present, 80% of sludge in my country has not been properly disposed of. However, its production is still increasing at a rate of 10-15% per year, and the sludge pollution is becoming more and more serious. The problem of sludge treatment has become a worldwide problem. [0003] Even though the current sewage treatment plant has concentrated the sludge, the water content of the wet sludge is still as high as 80%. Moreover, these sludges contain mercury, cadmium, pathogenic bacteria, parasites and other subst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F11/02C12R1/07C12R1/865C02F103/00
CPCC02F11/02C12N1/18C12N1/20Y02W10/20
Inventor 王慧荣梅荣武韦彦斐李明智张宇任旭峰
Owner ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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