A kind of culture medium for rapid growth of Dendrobium candidum in vitro

Abstract

The invention provides a culture medium for rapid growth of Dendrobium candidum in vitro, the culture medium is MS solid medium, and also includes 3-10g / L rhamnolipid, and 2-5g / L hydrophilic polymer material . The present invention adds a hydrophilic polymer material to the culture medium, which has a slow-release effect, can promote the uniform distribution of nutrients in the culture medium, adjust the release speed of nutrients, meet the continuous and balanced absorption of nutrients by seedlings, and further promote the development of iron sheet The growth and development of Dendrobium; Adding rhamnolipid in the medium can inhibit fungi and bacteria, and can effectively reduce the pollution of the medium.

Description

technical field [0001] The invention relates to a culture medium for dendrobium candidum, in particular to a culture medium for rapid growth of dendrobium candidum in vitro. Background technique [0002] Dendrobium officinale is one of the rarest Chinese herbal medicines in my country. It is known as the "gold in medicine". Liver and other health effects. [0003] Wild Dendrobium officinale has strict requirements on the natural ecological conditions, the natural reproduction rate is extremely low, the growth is slow, and the resources are scarce. Due to long-term excavation, the ecological environment has deteriorated, and many rare species are on the verge of extinction. It has been listed as a rare and endangered medicinal plant under national key protection. , which has seriously affected the development and utilization of Dendrobium officinale. [0004] Generally, conventional MS medium is used in the production of Dendrobium candidum. After the seedlings of Dendrobium...

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Problems solved by technology

[0003] Wild Dendrobium officinale has strict requirements on the natural ecological conditions, the natural reproduction rate is extremely low, the growth is slow, and the resources are scarce. Due to long-term excavation, the ecological environment has deteriorated, and many rare species are on the verge of extinction. It has been listed as a rare and endangered medicinal plant under national key protection. , which has seriously affected the development and utilization of Dendrobium officinale

[0004] Generally, conventional MS medium is used in the production of Dendrobium candidum. After the seedlings of Dendrobium candidum are inoculated into the medium, due to the high initial concentration of various nutrients in the medium, the speed of absorption of nutrients by the seedlings is relatively slow. Leaves turn yellow, seedlings shrink, and even cause seedlings to die

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