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A kind of indoor rapid cultivation method of wild barley

A breeding method and barley technology, applied in the field of indoor rapid cultivation of wild barley, can solve the problems of low seed germination rate, weak plants, slow growth, etc., and achieve the effects of eliminating salt tolerance and drought resistance, neat emergence and vigorous growth

Inactive Publication Date: 2017-03-08
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because it belongs to wild germplasm, the biggest challenge when cultivating it indoors is low seed germination rate, slow growth, and weak plants; if no special treatment is done, its germination rate can only reach 30-50% under normal conditions. It takes 40-50 days to grow to the 4-leaf stage, and the leaves are small and yellow, which is not conducive to scientific research as a research material
[0003] Studies have shown that, as a halophytic wild barley, an appropriate amount of NaCl can promote the germination, growth, and photosynthesis of wild barley seeds, but there is no NaCl-based, suitable for rapid indoor germination of wild barley, and enhances the photosynthesis of wild barley seeds. An effective way to grow plants and improve the uniformity of seedlings

Method used

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  • A kind of indoor rapid cultivation method of wild barley
  • A kind of indoor rapid cultivation method of wild barley
  • A kind of indoor rapid cultivation method of wild barley

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for indoor rapid cultivation of wild barley includes the following steps:

[0020] 1) Remove the seeds of wild barley, select neat seeds and treat them at -20℃ for 48h. After washing the wild barley seeds with distilled water, use 100mg / L of gibberellin before seed germination at a low temperature of 0-4℃. deal with;

[0021] 2) The pre-treated wild barley seeds obtained in step 1) are simply rinsed with distilled water, and then evenly sprinkled on the seed rack of the seed germination box for germination. The nutrient solution in the germination box is a nutrient solution containing 5mmol / L NaCl; germination The indoor conditions are 24 / 18℃, dark, and wild barley seeds can germinate after 3 to 5 days, and the germination rate can reach 82%;

[0022] 3) Change the seedlings obtained in step 2) to light culture, 25℃, 16h light and 16℃, 8h without light, alternately, the light intensity is 600μmol / (m 2 ·S), the culture medium is hydroponics, and the composition is a n...

Embodiment 2

[0027] An indoor rapid cultivation method for wild barley, the steps are the same as in Example 1, except that the relevant temperature and NaCl concentration are adjusted, and it mainly includes the following steps:

[0028] 1) Remove the seeds of wild barley, select uniform seeds and treat them at -18℃ for 48 hours. After washing the wild barley seeds with distilled water, use 150mg / L of gibberellin for seed germination at a low temperature of 0-4℃ Pretreatment

[0029] 2) The pre-treated wild barley seeds obtained in step 1) are simply rinsed with distilled water, and then evenly sprinkled on the seed rack of the seed germination box for germination. The nutrient solution in the germination box is a nutrient solution containing 10mmol / L NaCl; for germination The indoor conditions are 26℃16h / 16℃8h, dark, the wild barley seeds can germinate after 3 to 5 days, and the germination rate can reach 85%;

[0030] 3) Change the seedlings obtained in step 2) to light culture, 24℃, 16h ligh...

Embodiment 3

[0035] An indoor rapid cultivation method for wild barley, the steps are the same as in Example 2, except that the relevant temperature, time and NaCl concentration are adjusted, and it mainly includes the following steps:

[0036] 1) Remove the seeds of wild barley, select uniform seeds and treat them at -16℃ for 24 hours. After washing the wild barley seeds with distilled water, use 200mg / L of gibberellin for seed germination at a low temperature of 0-4℃ Pretreatment

[0037] 2) The pre-treated wild barley seeds obtained in step 1) are simply rinsed with distilled water, and then evenly sprinkled on the seed rack of the seed germination box for germination. The nutrient solution in the germination box is a nutrient solution containing 15mmol / L NaCl; for germination The indoor conditions are 20℃16h / 16℃8h, dark, the wild barley seeds can germinate after 3 to 5 days, and the germination rate can reach 80%;

[0038] 3) Change the seedlings obtained in step 2) to light culture, 24℃, 16...

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Abstract

The invention discloses a hordeum brevisubulatum indoor quick cultivation method. The hordeum brevisubulatum indoor quick cultivation method comprises performing pre-germination treatment on low-temperature-treated hordeum brevisubulatum seeds through gibberellin, washing and then germinating in a nutrient solution containing NaCl, and performing light culture on germinated seedlings inside a hydroponic culture medium; after the germinated seedlings grow for 14-20 days, transplanting uniform seedlings to an earth culture environment; irrigating the seedlings with a doubled nutrient solution containing NaCl, and performing light culture on the seedlings. The hordeum brevisubulatum indoor quick cultivation method has the advantage of solving the problem of low germinating speed, weak plant and slow growth of the wild forage of hordeum brevisubulatum indoors, being capable of rapidly breaking dormancy of the hordeum brevisubulatum seeds and achieving a germination rate of higher than 80% within 3-5 days, obtaining uniform germination, and ensuring that the seedlings grow vigorously in the nutrient solution containing NaCl. The hordeum brevisubulatum indoor quick cultivation method is significant to the aspects such as indoor study of poor biological repeatability of biological characteristics and salt-enduring and drought-resistant performance of the hordeum brevisubulatum to eliminating poor plant growth and low growth speed.

Description

Technical field [0001] The invention relates to the field of plant cultivation, in particular to an indoor rapid cultivation method of wild barley. Background technique [0002] Wild barley (Hordeum brevisubulatum (Trin.) Link) belongs to the perennial herbaceous barley of the Gramineae family. As one of the most salt-tolerant halophytes in the Gramineae Triticeae family, it is closely related to barley and wheat. . It has the characteristics of high yield, good palatability, and high nutritional value, and is used as the dominant germplasm resource for new varieties of crops and forage hybrids. At present, hybrid experiments have been carried out with forages such as Elymus dahurica, and the munition No. 1 wild barley with high production performance and strong salt resistance has been cultivated. But because it belongs to wild germplasm, the biggest challenge when cultivating indoors is the low seed germination rate, slow growth, and weak plants. Without special treatment, it...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01C1/00A01G31/00
Inventor 王春梅田福平王晓力杨红善杨晓张茜胡宇伍国强袁慧君王旭荣褚敏路远
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS