A kind of cultivation method of Cymbidium

A cultivation method, the technology of Cymbidium, applied in the field of cultivation of Cymbidium, can solve the problems of limiting protocorm propagation speed, variation, and affecting the quality of seedlings, so as to shorten the subculture time, increase the proliferation rate, reduce the The effect of training costs

Active Publication Date: 2017-11-10
MIANYANG XIANLONG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of cultivation method for Cymbidium phalanx in view of the problems that the current propagation of Cymbidium cymbidium by tissue culture technology inevitably produces variation, affects the quality of seedlings, and limits the propagation speed of protocorms. method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Huofenghuang is a very popular variety of Cymbidium, but during the tissue culture process, the protocorm-like proliferation rate is slow and the mutation rate is high. This variety is used as an example to illustrate the effect of this technology in actual production.

[0029] Configure liquid proliferation medium, wherein the concentration of each component is: 500mg / L (NH 4 ) 2 SO 4 , 250mg / L MgSO 4 ·7H 2 O, 525mg / L KNO 3 , 250mg / L KH 2 PO 4 、200mg / L Ca 3 (PO 4 ) 2 , 28mg / L Fe 2 (C 4 h 4 o 6 ) 3 2H 2 O, 7.5mg / L MnSO 4 4H 2 0. 20000mg / L sugar, adjust the pH value of the liquid proliferation medium to 5.8, and use a high-pressure steam sterilizer to sterilize for about 25 minutes.

[0030] Configure solid proliferation medium, wherein the concentration of each component is: 500mg / L (NH 4 ) 2 SO 4 , 250mg / L MgSO 4 ·7H 2 O, 250mg / L KH 2 PO 4 , 200mg / L Ca(NO 3 ) 2 4H 2 O, 28mg / L FeSO4 ·7H 2 O, 7.5mg / L MnSO 4 4H 2 O, 0.2mg / L 6-BA, 0.1mg / L NAA,...

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PUM

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Abstract

The invention discloses a method for cultivating Cymbidium cymbidium, aiming to solve the problem that when the current tissue culture technique is used to propagate Cymbidium cymbidium, variation inevitably occurs, which affects the quality of seedlings and limits the propagation speed of protocorms. The method comprises the following steps: using bud top clone tissue to induce protocorm-like, protocorm-like subculture multiplication, protocorm-like differentiation to produce adventitious buds, and cultivating strong adventitious buds to obtain seedlings. The invention can effectively solve the variation problem in the production of Cymbidium cymbidium seedlings, reduce the occurrence of variation, increase the value-added rate, shorten the subculture time by more than half, and increase the protocorm-like multiplication rate in a disguised form. It is determined that the invention increases the value-added rate of protocorm-like protocorms by 80-120%, and reduces the variation rate by 5-9%, effectively solving the contradiction between the value-added rate and the variation rate of the protocorm-like Cymbidium. The invention has important significance for rapid propagation of Cymbidium seedlings and industrialized large-scale production, and has good market prospect and application value.

Description

technical field [0001] The invention relates to the field of agriculture, in particular to the field of orchid cultivation, in particular to a method for cultivating Cymbidium cymbidium. The invention can effectively solve the variation problem in the cultivation of the Cymbidium cymbidium, and has good effect. Background technique [0002] Cymbidium grandifloruim (Cymbidium grandifloruim) is a large-flowered epiphytic species in the genus Cymbidium. It has rich and gorgeous colors, tall and straight flower branches, huge flowers, super long flowering period, light fragrance, and noble temperament. , has extremely high ornamental value and commodity value, and is deeply loved by people all over the world. [0003] Cymbidium has long been widely planted in Japan, South Korea, the United States, New Zealand, Europe, Taiwan and other places, and the Cymbidium industry in my country is also booming, with annual sales of more than one million pots. With the vigorous development...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李茜茜曾玉冰
Owner MIANYANG XIANLONG BIOTECH CO LTD
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