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A kind of method of isolation breeding of single flower of sunflower inbred line

A sunflower single and sunflower technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of time-consuming and laborious, shorten the breeding process of sunflower inbred lines, etc., and achieve the effect of shortening the breeding process.

Active Publication Date: 2017-06-16
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the lack of time-consuming and labor-intensive breeding methods of sunflower inbred lines single-pan self-bred breeding inbred lines in the prior art, the present invention provides a method for single-flower isolation breeding of sunflower inbred lines, using a self-set Sunflower inbred line single flower isolation breeding device, the method can shorten the selection process of sunflower inbred line

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Breeding of Xinshikui No. 9 restorer line XX2-u111111R

[0026] Xinshikui No. 9 is an edible sunflower hybrid with high yield, good comprehensive economic traits, and strong stress resistance selected by the applicant Xinjiang Academy of Land Reclamation Science. It was a hybrid combination of BH3-Ⅱ332333 and XX2-u111111 in 2007. In July 2012, it passed the approval of the Crop Variety Approval Committee of Xinjiang Uygur Autonomous Region, and the approval number was Xinshenshikui 2012 No. 12.

[0027] The characteristics of Xinshikui No. 9: the plant height is 188.3 cm, the number of leaves is 28, and the leaves are green. The ligulate flowers are yellow, with a large amount of tubular pollen. The diameter of the disk is 18.6 cm, and the inclination is grade 3. This variety is a mid-early maturing hybrid with a growth period of 104 days. The thousand-grain weight is 143 grams, and the kernel yield is 56.8%. The plants grow neatly and are resistant to lod...

Embodiment 2

[0034] Example 2: Breeding of Xinshikui No. 9 maintainer line BH3-Ⅱ332333B and sterile line BH3-Ⅱ332333A:

[0035] (1) Production of the single-flower isolation device: Cut the parchment paper into paper pieces with a size of 3cm×3cm, then roll the paper pieces into a cylinder with a diameter of about 0.4cm, glue the joints firmly with glue, and let them air dry for later use.

[0036] (2) Selection of single sunflower flowers: 1 day before the stigmas of Xinshikui No. 9 CMS line BH3-Ⅱ332333A and maintainer line BH3-Ⅱ332333B flowered, 10 stigmas were evenly selected on the sunflower disk, and the surrounding area of ​​each stigma was Remove the stigma with a knife as a sign.

[0037] (3) Isolation of single sunflower flowers: put the prepared parchment single flower isolation paper tube on the selected stigma, and fix it with hairpins.

[0038] (4) Pollination of single sunflower flowers: The single-flowered Xinshikui No. 9 maintainer line BH3-Ⅱ332333B was self-pollinated wit...

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Abstract

The invention provides a sunflower inbred line single flower isolation breeding method. A self-arranged sunflower inbred line single flower isolation breeding device is used for the breeding of the sunflower inbred line; parchment bags are used for producing single flower isolation devices, which sleeve on the chosen stigmas and fixed with hairpins; a sterile line and corresponding maintainer line pollination method is as below: the maintainer line of the single flower paternal, which is a male parent, is subjected to self pollination by using a medical swab, and then maintainer line pollen is taken by the medical swab, so as to pollinate the appropriate sterile line that is female; restorer pollination is as below: self-pollination of the restorer of a single flower is conducted by a medical swab, thereby establishing the sunflower inbred line single flower isolation breeding method. The method shortens the breeding process of sunflower inbred line by at least two generations compared with a conventional method, and has practical role and significance.

Description

technical field [0001] The invention relates to the technical field of plant propagation, in particular to the technical field of breeding of sunflower inbred lines. Background technique [0002] sunflower( Helianthus annuus L. ) is one of the five major economic crops in the world. It has the characteristics of salt-alkali resistance, drought resistance, and barrenness. It is also the main oil crop in northern my country. fields and saline-alkali land, so sunflower plays an important role in agricultural production. All countries in the world have generally carried out three-line research on sunflower strains, maintainer lines, and restorer lines in the study of sunflower heterogeneity utilization. my country has carried out research on sunflower breeding since the 1970s. [0003] The selection of sunflower inbred lines is the basis of sunflower hybrid breeding and the basic condition for cultivating excellent hybrids. Only by cultivating inbred lines with excellent traits...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 段维刘胜利陈寅初柳延涛王鹏李万云赵刚杨涛侯东升
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI