Maize gene zmravl1 and functional site and its use

Abstract

The present invention relates to maize gene ZmRAVL1 and functional site and use thereof. The present invention locates the functional site that controls the leaf angle phenotype to 240bp through fine mapping, and the insertion or deletion of this region produces different angle phenotypes. The present invention confirms that the improved inbred line by utilizing the excellent natural variation derived from the ruminant grass can increase the yield of maize under high planting density, and broadens the sources of excellent alleles available in plant breeding; the present invention adopts the genetic engineering technology (RNAi) It is confirmed that reducing the expression of ZmRAVL1 can affect the plant type, such as reducing the leaf angle, and provides excellent gene resources for genetic engineering breeding; the present invention generates more excellent alleles through gene editing technology, and greatly shortens the time of excellent alleles. The selection process provides a new idea for obtaining usable excellent alleles in breeding practice; the invention combines molecular assisted selection technology, can rapidly and accurately improve or generate excellent inbred lines, and provide excellent alleles for wide application. possible.

Description

technical field [0001] The invention belongs to the field of plant gene map-based cloning and molecular breeding, and particularly relates to a maize gene ZmRAVL1 and related functional sites and corresponding uses. Background technique [0002] Corn is the world's largest food crop, and now the world's total corn production has exceeded 1 billion tons. In China, like the United States, corn is mainly used as feed. However, China's corn acreage is similar to that of the United States, but yields and total yields are about 60% of those of the United States. The growth space of corn planting area in my country is close to the limit. What is more serious is that the harsh natural environment such as water shortage and frequent extreme weather is irreversible. Simply relying on increasing the sown area to improve my country's maize yield is already very limited. The fundamental way out is to fully tap the huge potential of maize genetic resources. The main way to ensure the co...

AI Technical Summary

Problems solved by technology

[0008] In summary, although some progress has been made in the genetic mapping of maize leaf angle, and breakthroughs have been made in the cloning of individual leaf angle genes, the research on the entire maize leaf angle is still mainly at the preliminary location. Horizontally, the genetic and molecular regulatory network established by maize leaf angle is far from elucidated

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