Portable quick detection method for pathogenic bacteria

Abstract

The invention discloses a portable quick detection method for pathogenic bacteria. The portable quick detection method comprises the following steps: step 1, adding magnetic beads into a to-be-detected substance, putting the to-be-detected substance into a heating groove, heating the to-be-detected substance at a constant temperature through a constant temperature control unit, putting a nucleic acid extraction module into the to-be-detected substance, and sucking out the magnetic beads adsorbing nucleic acid according to magnetic regions of the nucleic acid extraction module; step 2, heating the to-be-detected substance through a heater; step 3, emitting laser by a laser emitter, enabling laser to pass through laser via holes and irradiate on the to-be-detected substance, enabling a laser sensor to receive the laser from the laser via holes, analyzing the laser through a control circuit board, and displaying detection data of the to-be-detected substance on a display module. The portable quick detection method is economical, portable, and accurate and efficient in detection.

Description

technical field [0001] The invention relates to the field of automatic control, in particular to a portable rapid detection method for pathogenic bacteria. Background technique [0002] In recent years, due to the frequent occurrence of foodborne pathogenic microorganism poisoning incidents, the mutation or cross-border transmission of animal and plant pathogens, and the safety of genetically modified ingredients, biosafety issues have become more and more prominent. Detection technology and detection equipment in the field of biosafety have become one of the research hotspots. For example, in 2013, New Zealand's "botulism" milk powder incident and Germany's "poisonous cucumber" and "adulterated horse meat ingredients" incidents occurred, resulting in the deteriorating food safety situation at home and abroad. In this context, any rapid detection technology that is beneficial to pathogenic microorganisms and their genetic components has great application value and market pr...

AI Technical Summary

Problems solved by technology

Not to mention the sensitivity and accuracy of the above-mentioned commercial system, in terms of its weight and selling price, it is not suitable for on-site detection of food microorganisms

The real-time turbidimeter and other ordinary PCR instruments of Japan's Eiken Company can also be applied to the rapid detection of food microorganism genes, but they do not integrate nucleic acid extraction components, the degree of automation is low, and the equipment is cumbersome, so it is only suitable for laboratory testing.

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