Application of amylase in cigar fermentation and quality improvement

A cigar and amylase technology, applied in the field of tobacco processing, can solve the problems that flue-cured tobacco enzymatic preparations cannot be applied to cigars, low sugar-to-nitrogen ratio, etc., and achieve the effects of improving use value, increasing sugar/nitrogen ratio, and balancing pH.

Inactive Publication Date: 2015-09-09
HUBEI CHINA TOBACCO IND
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Due to the low sugar-to-nitrogen ratio of cigar tobacco raw materials, flue-cured tobacco enzyme preparations cannot be applied t

Method used

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  • Application of amylase in cigar fermentation and quality improvement
  • Application of amylase in cigar fermentation and quality improvement

Examples

Experimental program
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Example Embodiment

[0026] Example 1

[0027] For the application of amylase in cigar fermentation and upgrading, the steps are as follows:

[0028] The alpha-amylase and glucoamylase raw materials are dissolved in purified water to prepare a 10kg enzyme solution to obtain the enzyme activity in the enzyme solution: alpha-amylase is 80 U / ml and glucoamylase is 800 U / ml.

[0029] In the upper part of the above cigar core, the smoke is regenerated in vacuum and humidified until the moisture content of the tobacco leaves is 15%. The stem is first soaked in 7kg enzyme solution for 30 minutes, and then 3kg enzyme solution (the density of the enzyme solution is 10 3 kg / m 3 According to calculation, the volume of 3kg enzyme solution is 3000ml) spray evenly applied to 30kg tobacco leaves, that is, the ratio of enzyme solution spray mass to tobacco leaf mass is 1:10, and each gram of tobacco leaf (original tobacco) corresponds to the α- The amylase is 8U, and the glucoamylase is 80U. Let it stand for 24 hours, ...

Example Embodiment

[0031] Example 2

[0032] The alpha-amylase and glucoamylase raw materials are dissolved in purified water to prepare a 10kg enzyme solution to obtain the enzyme activity in the enzyme solution: alpha-amylase is 40 U / ml and glucoamylase is 560 U / ml.

[0033] In the upper part of the above cigar core, the tobacco is humidified under vacuum and humidified until the moisture content of the tobacco leaves is 16%. The stem is first soaked in 6.25kg enzyme solution for 30 minutes; then 3.75kg enzyme solution (the density of the enzyme solution is 10 3 kg / m 3 Calculation) The spray is evenly applied to 30kg tobacco leaves, that is, the ratio of the mass of the enzyme solution spray to the mass of the tobacco leaf is 1:8, and the corresponding α-amylase and glucoamylase in each gram of tobacco leaf (raw tobacco) are allocated to the enzyme solution spray. 70U; stand for 12h, after moisture balance, adjust the moisture content of tobacco leaves to 24%, put it into fermentation chamber for ar...

Example Embodiment

[0035] Example 3

[0036] The α-amylase and glucoamylase raw materials are dissolved in purified water to prepare a 10kg enzyme solution to obtain the enzyme activity in the enzyme solution: alpha-amylase is 60 U / ml, and glucoamylase is 720 U / ml.

[0037] In the upper part of the above cigar core, the smoke is vacuumed and humidified until the moisture content of the tobacco leaves is 15%. The stem is first soaked in 7.5kg enzyme solution for 30 minutes, and then 2.5kg enzyme solution (the density of the enzyme solution is 10 3 kg / m 3 Calculated) spray evenly applied to 30kg tobacco leaves, that is, the ratio of the quality of the enzyme solution spray to the quality of the tobacco leaf is 1:12, and the corresponding α-amylase in each gram of tobacco leaf (original tobacco) allocated to the enzyme solution spray is 5U, and the glucoamylase is 60U. After standing for 18h, after the moisture balance, adjust the moisture content of the tobacco leaves to 26%, then put them into the fer...

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Abstract

The invention discloses application of amylase in cigar fermentation and quality improvement. The application is characterized by comprising the following steps of (a) conducting vacuum humidifying on cigar tobacco leaves alcoholized naturally, soaking stems in an enzyme solution prepared by alpha-amylase, saccharifying enzyme and water or a buffer solution for 10-30 min, wherein the activity of the alpha-amylase in the enzyme solution is 40-80 U/ml, and the activity of the saccharifying enzyme is 400-800 U/ml; (b) evenly spraying the enzyme solution on the tobacco leaves, wherein the ratio of the enzyme solution to tobacco leaf quality is 1:8-12; (c) standing the tobacco leaves for 12-24 hours, regulating moisture content of the tobacco leaves after moisture balance for fermentation. The bitter taste of the tobacco leaves processed by the processing technology is obviously improved, the fragrance, smoke and taste features are improved, and quality improved.

Description

technical field [0001] The invention relates to the field of tobacco processing, in particular to the application of an amylase in fermenting and improving the quality of cigar tobacco. Background technique [0002] Starch-like carbohydrates exist in tobacco leaves, which on the one hand affect the combustibility of cigarettes, and on the other hand, starch will produce a burnt smell when smoking, which will have a negative impact on smoke. The residual starch in the raw tobacco is caused by incomplete starch conversion in the tobacco leaves. According to literature reports, the starch content in foreign tobacco leaves is about 1-2%, and the starch content in Chinese tobacco leaves is about 4-6%. [0003] At present, a small amount of starch remains in domestic tobacco leaves during the brewing process. Starch affects the burning speed and completeness of burning when smoking, and produces burnt smell, which affects the quality of smoking. Under certain conditions, starch c...

Claims

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Application Information

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IPC IPC(8): A24B15/20
CPCA24B15/20
Inventor 胡捷刘洋潘勇周斌
Owner HUBEI CHINA TOBACCO IND
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