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Application of amylase in cigar fermentation and quality improvement

A cigar and amylase technology, applied in the field of tobacco processing, can solve the problems that flue-cured tobacco enzymatic preparations cannot be applied to cigars, low sugar-to-nitrogen ratio, etc., and achieve the effects of improving use value, increasing sugar/nitrogen ratio, and balancing pH.

Inactive Publication Date: 2015-09-09
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low sugar-to-nitrogen ratio of cigar tobacco raw materials, flue-cured tobacco enzyme preparations cannot be applied to cigar tobacco raw materials. Therefore, the effect of some additives and amylase on cigar tobacco remains to be further studied.

Method used

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  • Application of amylase in cigar fermentation and quality improvement
  • Application of amylase in cigar fermentation and quality improvement

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The application of amylase in cigar tobacco fermentation and quality improvement, the steps are as follows:

[0028] Dissolve the raw materials of α-amylase and glucoamylase in purified water to prepare 10kg of enzyme solution. The enzyme activity in the enzyme solution is: 80U / ml for α-amylase and 800U / ml for glucoamylase.

[0029] Vacuum the upper part of the above-mentioned cigar filler, humidify the tobacco leaves until the moisture content is 15%, soak the handle in 7kg of enzyme solution for 30 minutes, and then add 3kg of enzyme solution (the density of the obtained enzyme solution is 10% of the density of pure water) 3 kg / m 3 Calculated, the volume of 3kg enzyme solution is 3000ml) and the spray is evenly applied to 30kg tobacco leaves, that is, the mass ratio of enzyme solution spray to tobacco leaves is 1:10, and the corresponding α- Amylase is 8U and glucoamylase is 80U. After standing still for 24 hours, after the moisture is balanced, the moisture content...

Embodiment 2

[0032] Dissolve the raw materials of α-amylase and glucoamylase in purified water to prepare 10kg of enzyme solution. The enzyme activity in the enzyme solution is: 40U / ml for α-amylase and 560U / ml for glucoamylase.

[0033] Vacuum remoisturize the tobacco on the upper part of the above-mentioned cigar filler, humidify until the moisture content of the tobacco leaves is 16%, first soak the stem in 6.25kg of enzyme solution for 30min; 3 kg / m 3 Calculation) spray is evenly applied on the 30kg tobacco leaves, that is, the mass ratio of the enzyme solution spray to the tobacco leaves is 1:8, and the α-amylase corresponding to the enzyme solution spray in each gram of tobacco leaves (original tobacco) is 5U, and the glucoamylase is 5U. 70U; stand still for 12 hours, after the water balance, adjust the moisture content of the tobacco leaves to 24%, put them into the fermentation room for artificial fermentation, the environment of the fermentation room is controlled at a temperature...

Embodiment 3

[0036] Dissolve α-amylase and glucoamylase raw materials in purified water, prepare 10kg of enzyme solution, and obtain the enzyme activity in the enzyme solution: α-amylase is 60U / ml, glucoamylase is 720U / ml.

[0037] Vacuum the upper part of the above-mentioned cigar filler, humidify the tobacco leaves until the moisture content is 15%, soak the stem in 7.5kg of enzyme solution for 30 minutes, and then add 2.5kg of enzyme solution (the density of the prepared enzyme solution is 10% of the density of pure water) 3 kg / m 3 ) spray evenly applied to 30kg of tobacco leaves, that is, the mass ratio of the enzyme solution spray to the tobacco leaves is 1:12, and the α-amylase corresponding to the enzyme solution spray in each gram of tobacco leaves (original tobacco) is 5U, and the glucoamylase is 5U. 60U. After standing still for 18 hours, after the water balance, adjust the moisture content of the tobacco leaves to 26%, and put them into the fermentation room for artificial ferm...

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Abstract

The invention discloses application of amylase in cigar fermentation and quality improvement. The application is characterized by comprising the following steps of (a) conducting vacuum humidifying on cigar tobacco leaves alcoholized naturally, soaking stems in an enzyme solution prepared by alpha-amylase, saccharifying enzyme and water or a buffer solution for 10-30 min, wherein the activity of the alpha-amylase in the enzyme solution is 40-80 U / ml, and the activity of the saccharifying enzyme is 400-800 U / ml; (b) evenly spraying the enzyme solution on the tobacco leaves, wherein the ratio of the enzyme solution to tobacco leaf quality is 1:8-12; (c) standing the tobacco leaves for 12-24 hours, regulating moisture content of the tobacco leaves after moisture balance for fermentation. The bitter taste of the tobacco leaves processed by the processing technology is obviously improved, the fragrance, smoke and taste features are improved, and quality improved.

Description

technical field [0001] The invention relates to the field of tobacco processing, in particular to the application of an amylase in fermenting and improving the quality of cigar tobacco. Background technique [0002] Starch-like carbohydrates exist in tobacco leaves, which on the one hand affect the combustibility of cigarettes, and on the other hand, starch will produce a burnt smell when smoking, which will have a negative impact on smoke. The residual starch in the raw tobacco is caused by incomplete starch conversion in the tobacco leaves. According to literature reports, the starch content in foreign tobacco leaves is about 1-2%, and the starch content in Chinese tobacco leaves is about 4-6%. [0003] At present, a small amount of starch remains in domestic tobacco leaves during the brewing process. Starch affects the burning speed and completeness of burning when smoking, and produces burnt smell, which affects the quality of smoking. Under certain conditions, starch c...

Claims

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Application Information

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IPC IPC(8): A24B15/20
CPCA24B15/20
Inventor 胡捷刘洋潘勇周斌
Owner HUBEI CHINA TOBACCO IND
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