Method for detecting lateral limitation area of living plant cytomembrane protein
A technology of laterally restricted area and cell membrane, which is applied in the field of detection of laterally restricted area of living plant cell membrane proteins, can solve the problems that have not been clearly reported, and achieve the effect of simple method and good repeatability
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[0038] Example 1. Detection of the laterally restricted area of phototropin phot1 on the cell membrane in Arabidopsis
[0039] The protein used to detect the laterally restricted area on the membrane is the Arabidopsis phototropin phot1, which is expressed on the epidermal cell membrane of the Arabidopsis hypocotyl. The phot1 tag was tagged using the green fluorescent protein GFP.
[0040] 1. Plant material
[0041] 1. Obtaining transgenic Arabidopsis
[0042] The Arabidopsis thaliana transformed into the fusion gene phot1-GFP under the phot1-5 mutant background used in the present invention comes from the document "Sakamoto and Briggs. Cellular and subcellular localization of phototropin 1. Plant Cell, 2002, 14:1723-1735" Publicly available and available to the public from the Institute of Botany, Chinese Academy of Sciences.
[0043] 2. Cultivation of Arabidopsis seeds
[0044] Transgenic Arabidopsis seeds were placed in a 1.5ml centrifuge tube, soaked in 2.5% (volume ...
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