Pharmaceutical composition of chloroquine and vincristine

A technology of vincristine and chloroquine, which is applied in the field of drug combination of chloroquine and vincristine, can solve the problems of high cost, high toxicity and side effects, and achieve the effect of low toxicity, significant effect and less dosage

Active Publication Date: 2015-09-16
HANGZHOU FANPU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, there are many tumors in children, and they are usually treated with surgery or chemotherapy. For example, the treatment methods for children with retinoblastoma are mainly surgical enucleation of the eyeball and local chemotherapy. Although the method of enucleation of the eyeball can cure the tumor, it has gradually been adopted due to the high cost. Cytotoxic drugs are currently the mainstream chemotherapy for various children with solid tumors; chemotherapy is limited to traditional cytotoxic drugs vincristine, carboplatin, cyclophosphamide, vincristine (VCR ) is the most commonly used, the microtubule inhibitor vincristine has a broad-spectrum anti-tumor effect, but has high toxicity and side effects; the lysosome inhibitor chloroquine (chloroquine, CQ) is a weak base that acts on lysosomes The acidic environment of the enzyme body degrades various proteins and organelles in the cell. It is used clinically to treat malaria. It is found that chloroquine can inhibit autophagy, a physiological mechanism and metabolic pathway of cell self-degradation, and autophagy is beneficial to the survival of tumor cells. Usually has a promoting effect, so chloroquine is used in experimental treatment and clinical trials of various malignant tumors, including breast cancer, melanoma, liver cancer, pancreatic cancer and other adult high-grade malignant tumors

Method used

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  • Pharmaceutical composition of chloroquine and vincristine
  • Pharmaceutical composition of chloroquine and vincristine
  • Pharmaceutical composition of chloroquine and vincristine

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Embodiment 1, a drug combination of chloroquine and vincristine, including chloroquine and vincristine, wherein the content of chloroquine is 2-40 μM, and the content of vincristine is 10 nM.

[0017] The specific implementation process is,

[0018] Retinoblastoma HXO-Rb44 cells were cultured in 1640 medium (containing 10% fetal bovine serum), inoculated in 96-well plates when they were growing well, and cultured with drugs for 24 hours. The relative number of cells was detected by CCK-8 method, and the cells were calculated. Inhibition rate of drugs on cells;

[0019] Cell line and culture method: retinoblastoma cell line HXO-Rb44, which is a suspension cell, cultured in 1640 medium containing 10% fetal bovine serum in an incubator at 37°C, containing 5% carbon dioxide and saturated water vapor;

[0020] Main reagents: chloroquine (CQ), vincristine (VCR); 40% acrylamide gel stock solution, Tris base, TEMED, SDS, protease inhibitors, protein loading standards, CCK8 kit...

Embodiment 2

[0025] Embodiment 2, a drug combination of chloroquine and vincristine, including chloroquine and vincristine, wherein the content of chloroquine is 5-50 μM, and the content of vincristine is 20nM;

[0026] The specific implementation process is,

[0027] Wilms tumor G401 cells were cultured in McCoy5A medium (containing 10% fetal bovine serum), and seeded in 96-well plates when the growth status was good. Add vincristine and chloroquine to co-culture for 24 hours, then detect the relative number of cells by the CCK-8 method, and calculate the inhibitory rate of the drug on the cells;

[0028] Cell line and culture method: Wilms tumor G401, the cells are adherent cells, cultured in McCoy5A medium containing 10% fetal bovine serum in a 37°C incubator, containing 5% carbon dioxide and saturated water vapor;

[0029] Main reagents: chloroquine (CQ), vincristine (VCR); 40% acrylamide gel stock solution, Tris base, TEMED, SDS, protease inhibitors, protein loading standards, CCK8 k...

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Abstract

The invention discloses a pharmaceutical composition of chloroquine and vincristine. The pharmaceutical composition comprises 2-40mu M of chloroquine, 10nM of vincristine, or 5-50mu M of chloroquine and 20nM of vincristine. Chloroquine and vincristine of two dosages can synergically kill and inhibit multiple children solid tumors including retinoblastoma and nephroblastoma, and can be used as a chemotherapeutic medicine. Compared with an ordinary medicine, the pharmaceutical composition is small in dosage, low in toxicity and remarkable in effect in killing and inhibiting retinoblastoma and nephroblastoma.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to a medicine combination of chloroquine and vincristine. Background technique [0002] At present, there are many tumors in children, and they are usually treated with surgery or chemotherapy. For example, the treatment methods for children with retinoblastoma are mainly surgical enucleation of the eyeball and local chemotherapy. Although the method of enucleation of the eyeball can cure the tumor, it has gradually been adopted due to the high cost. Cytotoxic drugs are currently the mainstream chemotherapy for various children with solid tumors; chemotherapy is limited to traditional cytotoxic drugs vincristine, carboplatin, cyclophosphamide, vincristine (VCR ) is the most commonly used, the microtubule inhibitor vincristine has a broad-spectrum anti-tumor effect, but has high toxicity and side effects; the lysosome inhibitor chloroquine (chloroquine, CQ) is a weak base that acts...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/475A61P35/00A61K31/4706
Inventor 陈希
Owner HANGZHOU FANPU BIOTECH CO LTD
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