Application and method of immunosuppressant rapamycin in improving the yield of pullulan polysaccharide from Aureobasidium pullulans
A technology of Aureobasidium pullulans and an immunosuppressant, which is applied to the application of the immunosuppressant rapamycin in improving the yield of A. pullulan polysaccharide, and the use of the immunosuppressant rapamycin to improve the A. pullulans In the field of pullulan polysaccharide production, it can achieve the effects of high yield, low fermentation cost and strong technical economy.
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Embodiment 1
[0019] Aureobasidium pullulans with the preservation number CCTCC NO: M 2012223 was inoculated into a shake flask containing 50 mL of seed medium (the volume of the shake flask was 500 mL), and cultured at a temperature of 25 ° C and a rotation speed of 220 rpm for 48 hour; then take the culture solution and inoculate it in a 500mL shake flask according to the inoculum size of 10%. 120 hours; then detect the content of polymalic acid and pullulan in the culture medium. The results showed that the yield of polymalic acid in this example was 48.01 g / L, the yield of pullulan was 38.6 g / L, and the cell amount of Aureobasidium pullulans was 19.5 g / L.
[0020] At the same time, a comparative test was carried out according to the same method as above, except that no rapamycin was added to the fermentation medium, and the contents of polymalic acid and pullulan in the culture liquid were detected after the fermentation was completed. The results showed that the yield of polymalic aci...
Embodiment 2
[0024]Aureobasidium pullulans with the preservation number CCTCC NO: M 2012223 was inoculated into a shake flask containing 50 mL of seed medium (the volume of the shake flask was 500 mL), and cultured at a temperature of 23 ° C and a rotation speed of 300 rpm for 96 hour; then take the culture solution and inoculate it into a 500mL shake flask according to the inoculum size of 10%. 150 hours; then detect the content of polymalic acid and pullulan in the culture medium. The results showed that the yield of polymalic acid in this example was 47.9 g / L, the yield of pullulan was 39.8 g / L, and the cell amount of Aureobasidium pullulans was 21 g / L.
[0025] At the same time, a comparative test was carried out according to the same method as above, except that no rapamycin was added to the fermentation medium, and the contents of polymalic acid and pullulan in the culture liquid were detected after the fermentation was completed. The results showed that the yield of polymalic acid ...
Embodiment 3
[0029] Aureobasidium pullulans with the preservation number CCTCC NO: M 2012223 was inoculated into a shake flask containing 50 mL of seed medium (the volume of the shake flask was 500 mL), and cultured at a temperature of 30 ° C and a rotation speed of 180 rpm for 36 hour; then take the culture solution and inoculate it in a 500mL shake flask according to the inoculum size of 10%. 96 hours; then detect the content of polymalic acid and pullulan in the culture medium. The results showed that the yield of polymalic acid in this example was 24.26 g / L, the yield of pullulan was 42 g / L, and the cell mass of Aureobasidium pullulans was 19 g / L.
[0030] At the same time, a comparative test was carried out according to the same method as above, except that no rapamycin was added to the fermentation medium, and the contents of polymalic acid and pullulan in the culture liquid were detected after the fermentation was completed. The results showed that the yield of polymalic acid in th...
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