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Simplified sugarcane test-tube plantlet rooting and seedling method

A technology for test-tube seedlings and sugarcane, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of reducing production efficiency, restricting production efficiency, poor quality of test-tube seedlings, etc., saving manpower, saving energy input, Easy to use effect

Active Publication Date: 2015-09-23
SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It takes more than 15 days for common sugarcane test-tube seedlings to take root, and the quality of test-tube seedlings is poor, and the production cost is high, which seriously restricts production efficiency and reduces production benefits.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0031] A simplified sugarcane test-tube plantlet rooting and seedling raising method is characterized in that it comprises the following steps:

[0032] (1) Prepare rooting medium: prepare rooting medium according to the following formula: 1 / 2MS macroelements + MS trace elements + MS iron salt + MS organic matter + naphthalene acetic acid 5-20 mg / L + sucrose 50 g / L;

[0033] (2) Sterilization of the rooting medium: the rooting medium was sterilized under high temperature and high pressure at 121° C. for 5 minutes;

[0034] (3) aseptic tube transfer: in the aseptic room or ultra-clean workbench, the sugarcane test-tube plantlet that has completed the multiplication culture cycle is transferred to the cooling rooting medium;

[0035] (4) rooting cultivation: the sugarcane test-tube plantlet in the rooting medium is moved into the cultivation chamber, and the control cultivation chamber environment is 25° C. of temperature, 80% of humidity, and 1200 lux of light intensity, and cu...

Embodiment 2

[0043] A simplified sugarcane test-tube plantlet rooting and seedling raising method is characterized in that it comprises the following steps:

[0044](1) Prepare rooting medium: prepare rooting medium according to the following formula: 1 / 2MS macroelements + MS trace elements + MS iron salt + MS organic matter + naphthalene acetic acid 5-20 mg / L + sucrose 50 g / L;

[0045] (2) Sterilization of the rooting medium: the rooting medium was sterilized under high temperature and high pressure at 121° C. for 10 minutes;

[0046] (3) aseptic tube transfer: in the aseptic room or ultra-clean workbench, the sugarcane test-tube plantlet that has completed the multiplication culture cycle is transferred to the cooling rooting medium;

[0047] (4) rooting cultivation: the sugarcane test-tube plantlet in the rooting medium is moved into the cultivation chamber, and the control cultivation chamber environment is 28°C of temperature, 70% of humidity, and 1000lux of light intensity, and culti...

Embodiment 3

[0055] A simplified sugarcane test-tube plantlet rooting and seedling raising method is characterized in that it comprises the following steps:

[0056] (1) Prepare rooting medium: prepare rooting medium according to the following formula: 1 / 2MS macroelements + MS trace elements + MS iron salt + MS organic matter + naphthalene acetic acid 5-20mg / L+ sucrose 50g / L; (2) rooting culture Base sterilization: sterilize the rooting medium at 121°C for 8 minutes under high temperature and high pressure;

[0057] (3) aseptic tube transfer: in the aseptic room or ultra-clean workbench, the sugarcane test-tube plantlet that has completed the multiplication culture cycle is transferred to the cooling rooting medium;

[0058] (4) rooting cultivation: the sugarcane test-tube plantlet in the rooting medium is moved into the cultivation room, and the environment of the control cultivation room is 30°C of temperature, 75% of humidity, and 2000lux of light intensity, and cultivates for 72 hours;...

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Abstract

The invention relates to a simplified sugarcane test-tube plantlet rooting and seedling method. The method comprises steps as follows: a sugarcane test-tube plantlet undergone a multiplication culture cycle is transferred to a rooting culture medium and cultured for 24-96 hours in a culture room; the rootless test-tube plantlet is divided, sterilized for 3-6 minutes and then planted in a planting pot containing a culture medium, and after a young leaf of the test-tube plantlet grows, a nursery bed management method is the same with a traditional nursery bed management method. According to the seedling method, the rootless test-tube plantlet replaces a traditional test-tube plantlet with a root for transplantation, autotrophic and heterotrophic rooting replaces traditional heterotrophic rooting, the rooting culture time of the sugarcane test-tube plantlet is shortened from 15 days to 1-4 days, the production efficiency is greatly improved, the production cost is reduced, and large-scale production and popularization of health sugarcane test-tube plantlets are facilitated.

Description

technical field [0001] The invention relates to a heterotrophic+photosynthetic autotrophic rooting method for plant test-tube seedlings, in particular to a simplified method for rooting and raising seedlings of sugarcane test-tube seedlings. Background technique [0002] Plant tissue culture generally goes through four stages, that is, primary culture (or establishment of a sterile culture system), subculture proliferation culture, rooting culture and seedling hardening and transplanting. At present, the sugarcane test-tube seedling rapid breeding technology that has been applied on a large scale in production also includes the above four stages. However, this traditional sugarcane test-tube plantlet production process is complex and requires high environmental conditions for cultivation, resulting in high cost of sugarcane tissue culture industrial production of test-tube plantlets, which has been unacceptable by the market and has limited the cultivation and production of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 何为中刘红坚翁梦苓陈荣发刘璐
Owner SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI
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