Novel bacterial biofilm culture device suitable for atomic force microscope (AFM) to perform in-situ detection

Abstract

The invention discloses a novel bacterial biofilm culture device suitable for an atomic force microscope (AFM) to perform in-situ and high-flux detection on the micro-nanostructure and biomechanical characteristics of biofilms. Biofilms have a wide application to the field of sewage treatment, and during the treatment process, the fluid shear stress has an impact on the physiological properties of bacteria, but the conventional bacterial biofilm culture device cannot meet the requirements of the AFM for real-time and in-situ detection. According to the novel bacterial biofilm culture device suitable for the atomic force microscope (AFM) to perform in-situ detection, provided by the invention, the micro-fluidic technique and the characteristic of the AFM are combined, so that the culture device, of which the culture part comprises a culture substrate, small culture rooms and a cover plate (8 small detection rooms are integrated on the same one culture substrate), is designed; the device can be embedded into an AFM detection platform to stimulate a sewage treatment system and perform in-situ detection on the micro-nanostructure and biomechanical characteristics of bacterial biofilms. The device is made of polycarbonate, can perform high temperature sterilization, and is reusable, economical and environment-friendly. A plurality of detection samples are integrated together, therefore, the detection time of the AFM is shortened, and the characteristic of high flux is achieved.

Description

technical field [0001] The invention relates to a novel bacterial biofilm cultivation device suitable for in-situ and high-throughput detection of biofilm micro-nano structures and biomechanical properties under sewage treatment conditions by an atomic force microscope. Background technique [0002] Bacterial biofilm is a microbial population in which bacterial cells adhere to each other and attach to the surface of objects. Bacteria are usually embedded in autocrine extracellular polymers (including DNA, proteins, and polysaccharides), and are widely present in water-containing objects (including industrial pipelines, medical devices and biological tissue) surfaces. [0003] Biofilm is widely used in the field of sewage treatment, and the research on the micro-nano structure and biomechanical properties of biofilm surface in the process of sewage treatment is of great significance. Bacteria show completely different physical and chemical properties when they grow in biofil...

AI Technical Summary

Problems solved by technology

Culture substrate materials can only be placed in fluidized chambers or microwell plates. After other tests, the substrate materials with biofilms are taken out for atomic force microscope observation, and the characteristics of biofilms cannot be characterized in situ.

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