A kind of solid phase extraction column and its application
A solid-phase extraction column and toxoid technology, which is used in measurement devices, instruments, scientific instruments, etc., can solve the problems of high price of immunoaffinity columns, inability to purify multiple substrates and multiple toxins at the same time, and achieve stable purification methods. , strong practicability, wide range of effects
Active Publication Date: 2016-08-24
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology
However, the commercialized immunoaffinity column is very expensive. Even the cheapest HLB column costs about 20 yuan per column, while the immunoaffinity column used in the pretreatment of the national standard GBT 23501-2009 even costs as much as 20 yuan per column. 80-100 yuan
Moreover, most commercial solid-phase extraction columns are aimed at the purification of a certain toxin or a certain matrix, and cannot achieve the purpose of purifying multiple toxins at the same time.
At present, there is still a lack of an inexpensive solid-phase extraction column that can be effectively used to simultaneously enrich and purify four type A trichothecenes in corn, wheat and rice
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Embodiment 1
[0030] Embodiment 1 prepares solid-phase extraction column
[0031] The schematic diagram of the solid phase extraction column is shown in figure 1 , mainly consists of four parts: packed column (volume 3mL), upper sieve plate (thickness 2mm), lower sieve plate (thickness 2mm) and filler (50mg, height about 5cm);
[0032] The filler is multi-walled carbon nanotubes (Nanjing Xianfeng Nano Material Technology Co., Ltd., model 8nm i.d., 10-30μm length, 500m 2 / g).
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Abstract
The invention provides a solid-phase extraction column. A filler of the solid-phase extraction column is a multiwall carbon nano tube. The solid-phase extraction column provided by the invention can be used for carrying out enrichment and purification on four A-type trichothecene toxins in crops of maize, rice, wheat and the like at the same time. The solid-phase extraction column provided by the invention is simple to prepare, is easy to operate, and has good enrichment and purification effects on the four A-type trichothecene toxins in the crops.
Description
technical field [0001] The invention relates to the technical field of agricultural product safety detection, in particular to a solid-phase extraction column and its application. Background technique [0002] Type A trichothecenes are a class of mycotoxins produced by Fusarium fungi, mainly including T-2 toxin (T-2), HT2 toxin (HT2), new solanisis fusarium enol (NEO) and There are four kinds of diacetyl simonenol (DAS). These four toxins can widely contaminate crops such as corn, wheat, and rice. After humans and animals eat such seriously contaminated grains, they can cause myocardial damage, gastrointestinal epithelial hemorrhage, skin tissue necrosis, hematopoietic tissue destruction, and immunosuppression. Nervous system disorder, cardiovascular system damage, etc., can cause death in severe cases. [0003] The existing purification methods for type A trichothecene toxoids are mainly solid-phase extraction column purification, and commercial solid-phase extraction col...
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IPC IPC(8): G01N30/60G01N30/08
Inventor 韩铮赵志辉董茂锋宋卫国聂冬霞司文帅
Owner SHANGHAI ACAD OF AGRI SCI