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Hollow mesoporous copper sulfide compound modified by hyaluronic acid and preparation method and application thereof

A technology of hyaluronic acid modification and mesoporous copper sulfide, which is applied in the field of medicine, can solve the problems of poor targeting, low biocompatibility, and weak water solubility of copper sulfide, and achieve good targeting and high biocompatibility , the effect of strong drug loading properties

Inactive Publication Date: 2015-09-30
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above situation, in order to overcome the defects of the prior art, the object of the present invention is to provide a hyaluronic acid-modified hollow mesoporous coppe

Method used

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  • Hollow mesoporous copper sulfide compound modified by hyaluronic acid and preparation method and application thereof

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Effect test

Embodiment 1

[0015] The hollow mesoporous copper sulfide complex modified by hyaluronic acid of the present invention is implemented by the following methods in specific implementation:

[0016] (1), 0.75g of CuSO 4 ·5H 2 Dissolve O in 200ml water, stir, add 6.0g polyvinylpyrrolidone, react at room temperature for 10-20min, add 1.2g sodium hydroxide, stir well, add dropwise 12ml hydrazine hydrate, stir for 5-10min, then add 1.29ml ammonium sulfate, stir 1h, centrifuge with ultrapure water at 15000rpm for 5-10min, wash with water until neutral, and freeze-dry for 48h to form hollow mesoporous copper sulfide nanoparticles;

[0017] (2) Add 200 mg of hollow mesoporous copper sulfide nanoparticles to 200 ml of ultrapure water, ultrasonically disperse for 10 to 30 minutes, then add 400 mg of mercaptoethylamine under stirring, stir for 24 hours, and centrifuge at 15,000 rpm for 5 to 10 minutes to obtain a precipitate. Redissolve in water, then centrifuge at 15000rpm for 5-10min to obtain a pre...

Embodiment 2

[0021] The hollow mesoporous copper sulfide complex modified by hyaluronic acid of the present invention is implemented by the following steps:

[0022] (1), 0.7g of CuSO 4 ·5H 2 Dissolve O in 180ml of water, stir, add 5.5g of polyvinylpyrrolidone, react at room temperature for 10-20min, add 1.1g of sodium hydroxide, stir well, add dropwise 10ml of hydrazine hydrate, stir for 5-10min, then add 1.2ml of ammonium sulfate, stir 1h, centrifuge with ultrapure water at 15000rpm for 5-10min, wash with water until neutral, and freeze-dry for 46-50h to form hollow mesoporous copper sulfide nanoparticles;

[0023] (2) Add 180 mg of hollow mesoporous copper sulfide nanoparticles to 180 ml of ultrapure water, disperse ultrasonically for 10 to 30 minutes, then add 360 mg of mercaptoethylamine under stirring, stir for 24 hours, and centrifuge at 15,000 rpm for 5 to 10 minutes to obtain a precipitate. Redissolve in water, then centrifuge at 15000rpm for 5-10min to obtain a precipitate, whi...

Embodiment 3

[0027] The hollow mesoporous copper sulfide complex modified by hyaluronic acid of the present invention can also be realized by the following methods:

[0028] (1), 0.8g of CuSO 4 ·5H 2 Dissolve O in 220ml water, stir, add 6.5g polyvinylpyrrolidone, react at room temperature for 10-20min, add 1.3g sodium hydroxide, stir well, add 14ml hydrazine hydrate dropwise, stir for 5-10min, then add 1.4ml ammonium sulfate, stir 1h, centrifuge with ultrapure water at 15000rpm for 5-10min, wash with water until neutral, and freeze-dry for 46-50h to form hollow mesoporous copper sulfide nanoparticles;

[0029](2) Add 220 mg of hollow mesoporous copper sulfide nanoparticles to 220 ml of ultrapure water, ultrasonically disperse for 10-30 minutes, then add 440 mg of mercaptoethylamine under stirring, stir for 24 hours, and centrifuge at 15,000 rpm for 5-10 minutes to obtain a precipitate. Redissolve in water, then centrifuge at 15000rpm for 5-10min to obtain a precipitate, which is then red...

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Abstract

The invention relates to a hollow mesoporous copper sulfide compound modified by hyaluronic acid and a preparation method and application thereof, and the problems that the water solubility of copper sulfide is poor, the targeting property is poor, and the biological compatibility is low can be effectively solved. The method comprises the steps that hollow mesoporous copper sulfide nanoparticles are bonded to the surfaces of copper sulfide nanoparticles, mercaptoethylamine are connected to the bonded copper sulfide nanoparticles, hyaluronic acid modification is conducted through amido bond reactions, and the hollow mesoporous copper sulfide compound modified by the hyaluronic acid is formed; the particle diameter ranges from 50 nm to 250 nm, the mass ratio of the hyaluronic acid to the hollow mesoporous copper sulfide is 1-3 to 3, and the molecular weight of the hyaluronic acid ranges from 40 kDa to 400 kDa. According to the hollow mesoporous copper sulfide compound modified by the hyaluronic acid and the preparation method and application thereof, the synthesis technology is easy, the non-targeting problems of a traditional light thermal therapy technology and a chemotherapy technology are solved, and damage to normal tissues is reduced in the therapeutic process; the hollow mesoporous copper sulfide compound modified by the hyaluronic acid and the preparation method and application thereof have the advantages of being efficient, controllable, strong in drug loading property, high in biological compatibility, good in targeting property, low in cost and the like, and the economic and social benefits are great.

Description

technical field [0001] The invention relates to medicine, in particular to a hyaluronic acid-modified hollow mesoporous copper sulfide compound and its preparation method and application. Background technique [0002] At present, as a safe and efficient pharmaceutical carrier, nanomaterials show broad development space in the treatment of tumors. As carrier materials, they should have the characteristics of small particle size, low toxicity, good biocompatibility, and stable performance. . Hollow mesoporous copper sulfide has a unique cage-like structure, outstanding drug-loading capacity, small particle size, stable physical and chemical properties, and low toxicity to organisms. Its water solubility and targeting can be enhanced by modifying some groups. It is beneficial for the carrier to flow with the blood in the living body to reach the tumor site, reduce the damage to normal tissues, and improve the therapeutic effect. [0003] Copper sulfide nanoparticles, as a new...

Claims

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Application Information

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IPC IPC(8): A61K47/36A61K47/48A61K31/728A61K41/00A61P35/00A61K49/00
Inventor 冯倩华侯琳张振中张媛媛单晓宁
Owner ZHENGZHOU UNIV
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