Method for determining buoyancy contribution of space between gas vesicle and grouped cells of cyanobacteria
A technology of colony cells and pseudo-ghost cells, which is applied in the field of measuring the buoyancy contribution of cyanobacterial pseudo-vacuoles and interspace between colony cells, and achieves the effect of high detection accuracy and simple method.
Inactive Publication Date: 2015-09-30
CHINA INST OF WATER RESOURCES & HYDROPOWER RES
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During the resuscitation process, pseudovacua are considered to be the buoyancy-providing factors for the resuscitation of algal cells, but the applicant's recent research results found that the intercellular space of the cyanobacteria population can also provide buoyancy for the algal population, and the intercellular space of the algal population is very likely It is the key factor for the algae population to float up, and this research result is the first report
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[0027] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0028] In the embodiment of the present invention, a method for measuring the buoyancy contribution of cyanobacterial pseudovacuoles and interstitial cells of the population, the specific method is:
[0029] (1) Take 1000ml of lake water in the field lake, filter through a 0.45um glass fiber membrane, remove the cyanobacteria cells, and obtain water without cyanobacteria cells for use;
[0030] (2) Select 10ml cyanobacteria bloom sample A in the field;
[003...
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The invention discloses a method for determining buoyancy contribution of space between gas vesicle and grouped cells of cyanobacteria. The method comprises the following specific steps: (1) taking a water body of 1000ml from a lack in the field, and filtering the water body through a glass fiber membrane of 0.45 micron to remove cyanobacteria cells, thereby obtaining a water body without cyanobacteria cells for later use; (2) selecting a cyanobacterial bloom sample A of 10ml in the field; (3) adding a cyanobacterial bloom sample A of 1ml into a water body of 99ml without cyanobacteria cells to obtain mixed liquor B; and (4) processing the mixed liquor B through ultrasonic waves with frequency being 45Hz for 5 minutes to obtain mixed liquor C. The method can be used for accurately determining buoyancy contribution of a space between gas vesicle and grouped cells of cyanobacteria during a floating process of cyanobacteria cells, is simple, and is high in detection precision.
Description
technical field [0001] The invention relates to a method for measuring the buoyancy contribution of cyanobacteria pseudovacuates and the space between group cells in the process of algae cell floating, in particular to a method for measuring the buoyancy contribution of cyanobacteria pseudovacua and the space between group cells. Background technique [0002] Cyanobacterial blooms have become one of the serious environmental problems faced by lakes and reservoirs. The formation mechanism of cyanobacterial blooms is divided into processes such as overwintering, recovery, biomass increase, and aggregation. During the resuscitation process, pseudovacua are considered to be the buoyancy-providing factors for the resuscitation of algal cells, but the applicant's recent research results found that the intercellular space of the cyanobacteria population can also provide buoyancy for the algal population, and the intercellular space of the algal population is very likely It is the k...
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IPC IPC(8): G01N33/00
Inventor 张永生彭文启吴文强蔚辉
Owner CHINA INST OF WATER RESOURCES & HYDROPOWER RES