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High activity Pleurotus eryngii liquid spawn and fermentation method thereof

A technology of liquid strains and highly active apricots, which is applied in botany equipment and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problem of shortening the age of strains, small diameter of mycelium balls, and the gap in multiplication algebra and other problems, to achieve the effect of shortening the age of the bacteria and the gap of the multiplication algebra, the diameter of the mycelium ball is small, and the distribution is uniform

Inactive Publication Date: 2015-10-07
TIANJIN ZHONGTIAN JINGKE SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem solved by the present invention is to overcome the defects of the existing Pleurotus eryngii liquid strains prepared by aerated fermentation, and the scientific compounding in the medium of each stage of the strain cultivation process can improve the cold resistance, heat stress and immunity of the strains. The active Chinese herbal medicine extract and the adaptive raw material sawdust powder are added inoculated in stages and fermented at variable temperatures to shorten the age and proliferation algebra gap of the strains, and the coated chitosan is added in a timely manner to adjust the viscosity of the fermentation medium. Cultivate a Pleurotus eryngii liquid strain with strong fermented hyphae, high activity, small mycelium ball diameter, high density, uniform distribution, and short fermentation period

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 raw material preparation

[0052] 1. Preparation of Chinese herbal medicine extract:

[0053] The preparation method of the Chinese herbal medicine extract comprises the steps of: weighing 30 parts of astragalus, 30 parts of dried ginger, 30 parts of tangerine peel, 25 parts of bezoar, 25 parts of paeonol, 20 parts of licorice, and 18 parts of anemarrhena in parts by weight. 18 parts of honeysuckle, 17 parts of Houttuynia cordata, 15 parts of red peony, 15 parts of scutellaria baicalensis, 10 parts of Chuanxiong, and 8 parts of angelica; put them in an ultrasonic cleaner filled with 0.2% sodium bicarbonate solution for 12 minutes at 200W and 30KHz, Drain, crush the above-mentioned Chinese herbal medicine to a particle size of less than 2 mm, put it in a container and mix evenly and add 5 times the weight of water to obtain a mixed material, control the temperature at 80°C for 3 hours, then lower the temperature to 45°C, and adjust the pH value with lactic a...

Embodiment 2

[0061] A method for fermenting liquid strains of Pleurotus eryngii with high activity, comprising the steps of:

[0062] 1) Take the preserved Pleurotus eryngii slant strain 0.2cm 2 Inoculate 2 pieces of the strain block of the above-mentioned bacteria on the plate medium for activation, and culture at a constant temperature of 25°C for 10 days, and activate twice in this way to obtain plate seeds;

[0063] 2) Use a puncher with a diameter of 0.5 cm to intercept the flat seed 0.2 cm 2 3 pieces of the strain block were inserted into a 250mL Erlenmeyer flask, and the amount of liquid seed culture medium was 100mL. They were cultured at a constant temperature of 25°C for 2 days, then placed in a constant temperature shaker, and shaken at 25°C and 100r / min for 3 days. liquid seeds;

[0064] 3) The liquid seeds were inoculated in a 500 mL shake flask with an inoculum amount of 10%, and the liquid volume of the shake flask culture medium was 250 mL, and cultured at 25° C. and 150 ...

Embodiment 3

[0075] A method for fermenting liquid strains of Pleurotus eryngii with high activity, comprising the steps of:

[0076] Step 1) to 4) with embodiment 2;

[0077] 5) First inoculate the seeds of the seed tank in a 30L fermenter with an inoculum amount of 8%, and ferment at a constant temperature of 30°C and an aeration rate of 6L / min for 12 hours, and then cool down to 18°C ​​at a rate of 0.3°C / min. ℃, add 7g coated chitosan and 900mL seed tank seeds into the fermenter, ferment at constant temperature for 10h under the condition of ventilation rate 6L / min, and finally raise the temperature to 24℃ at the rate of 0.2℃ / min, and then increase the temperature at the rate of 0.2℃ / min. Ferment at constant temperature for 45 hours under certain conditions to obtain liquid strains of Pleurotus eryngii with high activity;

[0078] Described plate medium composition is the same as embodiment 2;

[0079] The liquid seed medium consists of: glucose 30g / L, yeast extract juice 5g / L, pepton...

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Abstract

Belonging to the technical field of Pleurotus eryngii cultivation, the invention discloses a high activity Pleurotus eryngii liquid spawn and a fermentation method thereof. Scientific compounding with a Chinese herb extract able to improve spawns' resistance to cold and heat stress, immunity and sundry bacteria resistant capacity is carried out in each stage medium of liquid seeds, shake flask seeds, seed tank seeds and fermentation tank liquid spawns, at the same time scientific compounding with sawdust powder having raw material adaptability and defoaming agent functions is performed, and by means of supplemental inoculation and subsection temperature-changing fermentation, the spawn age and proliferation algebra gap are shortened, fed-batch of coated chitosan with special functions is conducted in proper time to culture the Pleurotus eryngii liquid spawn characterized by robust fermentative hyphae, high activity, small mycelium pellet diameter, large density and uniform distribution. The mycelium pellet density is 4.5-5.5*10<5> / L, the mycelium pellet diameter is 0.5-0.7mm, the mycelium pellet dry weight is 90-100g / L, the germination time is 4-5h, and the exopolysaccharide is 3.0-3.5g / L.

Description

technical field [0001] The invention relates to the technical field of Pleurotus eryngii cultivation, in particular to a liquid strain of Pleurotus eryngii with high activity and a fermentation method thereof. Background technique [0002] Pleurotus eryngii (PleurotuseryngiiQuel.), also known as Pleurotus eryngii, has the advantages of thick flesh and crisp texture, especially its dense, firm, milky white and refreshing stipe. It is called "king of oyster mushrooms" and "scallop mushrooms". , with almond fragrance and abalone taste, suitable for fresh-keeping and processing, and is deeply loved by people. It is rich in nutrients, rich in protein, carbohydrates, vitamins and minerals such as calcium, magnesium, copper, zinc, etc., which can improve human immune function. It has the effects of anti-cancer, lowering blood fat, moisturizing the stomach and beautifying the human body, and has extremely high cultivation value and commercial value. [0003] At present, the cultiva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/04C12R1/645
CPCC12N1/14A01G18/00
Inventor 李建树
Owner TIANJIN ZHONGTIAN JINGKE SCI & TECH
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