Extraction method of antibacterial peptide of rice field eel

An extraction method and antimicrobial peptide technology, applied in the field of eel antimicrobial peptide extraction, to achieve the effects of stable antibacterial activity, strong killing effect, and strong heat resistance and freeze-thaw resistance

Inactive Publication Date: 2015-10-14
CHONGQING DOUHAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, antimicrobial peptides fro...

Method used

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Embodiment

[0021] A method for extracting rice field eel antimicrobial peptide of the present embodiment is characterized in that it comprises the following specific steps:

[0022] 1) Preparation of crude product:

[0023] (1) Take the liver and intestines of fresh eels, wash them with sterilized saline, weigh them, freeze them and cut them into pieces;

[0024] (2) Add 5% acetic acid at a ratio of 1:1 (W / V);

[0025] (3) Use a tissue masher to mash 3 times, each time for 2 minutes;

[0026] (4) 100°C water bath for 10 minutes, then rapidly cooled, centrifuged at 8000 rpm for 30 minutes to discard the precipitate, and collect the supernatant;

[0027] (5) Put the supernatant on ice and ultrasonically break it 10 times, 3s each time; stir and extract at 4°C for 24 hours, centrifuge at 8000r / min for 30min, and take the supernatant;

[0028] (6) Mix the precipitate with an equal volume of 5% acetic acid, stir and extract again, and centrifuge to get the supernatant;

[0029] (7) Combin...

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PUM

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Abstract

The invention discloses an extraction method of antibacterial peptide of rice field eel. With the liver and intestines of rice field eel as raw materials, the antibacterial peptide of rice field eel with molecular weight of about 6,500Da is obtained by the steps of preparing a crude product of antibacterial peptide and purifying the crude product of antibacterial peptide; and the antibacterial peptide has the advantages of strong resistance to heat and freeze thawing, stable antibacterial activity and relatively strong killing effect on aeromonas hydrophila, escherichia coli and staphylococcus aureus.

Description

technical field [0001] The invention relates to an extraction method of antibacterial peptide, in particular to an extraction method of rice field eel antibacterial peptide. Background technique [0002] Antibacterial skin is a new generation of antibacterial agent discovered in recent years, which plays an important role in the immune function of the body. Research on antibacterial skin in aquatic animals started relatively late. Antimicrobial peptide research in aquatic animals began in 1988. Japanese scholar Nakamura et al. purified Tachyplesin from Asian horseshoe crab (Tachypleustridentatus) by acid extraction. Later, it was reported in shellfish and fish. However, antimicrobial peptides from rice field eel have been rarely reported so far. [0003] Monopterus albus, scientific name: Monopterus albus, is one of the main economic aquatic products in my country. It is rich in nutrients and has the reputation of "matching ginseng with rice field eels in Slight Heat". Th...

Claims

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Application Information

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IPC IPC(8): C07K14/46C07K1/36C07K1/16
CPCC07K14/461
Inventor 陈春林宁发子宁成勇
Owner CHONGQING DOUHAO BIOTECH
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