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A kind of preparation method of Bacillus coagulans liu-g1 viable bacteria preparation producing neutral protease

A technology of bacillus coagulans and neutral protease, applied in the field of preparation of live bacillus coagulans Liu-g1 preparation

Active Publication Date: 2018-08-03
BEIJING UNIV OF AGRI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method for preparing live bacterial preparations from Bacillus coagulans producing neutral protease has not yet been reported in relevant literature and patents at home and abroad.

Method used

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  • A kind of preparation method of Bacillus coagulans liu-g1 viable bacteria preparation producing neutral protease
  • A kind of preparation method of Bacillus coagulans liu-g1 viable bacteria preparation producing neutral protease
  • A kind of preparation method of Bacillus coagulans liu-g1 viable bacteria preparation producing neutral protease

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Embodiment 1

[0027] Embodiment 1, the preparation of the bacillus coagulans Liu-g1 live bacterial preparation that produces neutral protease

[0028] 1. Activation of strains

[0029] The slant or plate culture of Bacillus coagulans Liu-g1 was inoculated into a sterilized 5mL BHI medium test tube, and cultured on a shaker at 45°C with a rotation speed of 150r / min for 12h to activate the culture for one generation. Then inoculum with 2% inoculum was transplanted into a sterilized 5mL basal medium test tube, and cultured on a shaker at 37°C with a rotation speed of 150r / min for 48h to activate and cultivate the second generation to obtain activated strains.

[0030] The formula of Bacillus coagulans Liu-g1 basal medium is (mass volume ratio concentration, W / V): 1% glucose, 1% peptone, 0.2% NaCl, 1000 mL distilled water, pH 7.0.

[0031] 2. Optimization of fermentation medium formula

[0032] (1) Single factor multi-level test to determine the fermentation medium formula

[0033] Determina...

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Abstract

The invention relates to a preparation method of a neutral protease-producing Bacillus coagulans (Bacillus coagulans) Liu-g1 (CGMCC No. 10790) live bacterial preparation, which is suitable for the production of functional live bacterial preparations for feeding in livestock and poultry aquaculture . The present invention utilizes a neutral protease-producing Bacillus coagulans Liu-g1 strain screened from traditional cheese, and prepares a live bacterial preparation with a high number of viable bacteria through processes such as activation, expanded culture, high-density fermentation, centrifugal concentration, and freeze-drying. . In the present invention, by optimizing the fermentation medium and fermentation conditions, the number of live bacteria of the Liu-g1 strain can reach more than 9.0×109CFU / mL by fermentation in a full-automatic fermenter, and the number of live bacteria of the live bacteria preparation after freeze-drying can reach 1011CFU / g or more. The living bacteria preparation prepared by the invention has low price and high content of living bacteria, can be used as biological additives and microecological preparations for poultry and livestock feed, and has the characteristics of increasing animal growth speed, saving feed consumption, reducing morbidity, reducing feeding costs and the like.

Description

technical field [0001] The invention relates to a preparation method of a neutral protease-producing bacillus coagulans Liu-g1 live bacterial preparation, which is suitable for the production of functional microecological preparations for livestock and poultry breeding. Background technique [0002] Feed microecological preparations are complex biological preparations that are fermented, purified, dried and refined by adopting animal intestinal beneficial microorganisms approved by the Ministry of Agriculture. They are green biological additives that reduce or replace the use of antibiotics. [0003] In 1989, the US Food and Drug Administration (FDA) and the American Association of Feed Control Officials published a list of 42 microbial strains that can be directly fed and generally considered safe, including Bacillus coagulans. The feed additives released by the Ministry of Agriculture of my country in the Catalog of Feed Additives (2013) include Bacillus coagulans, which c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/07
CPCC12N1/20C12N1/205C12R2001/07
Inventor 刘慧张红星谢远红郭茜高秀芝熊利霞金君华
Owner BEIJING UNIV OF AGRI