Systems, apparatus, and methods for sorting particles

A detection system, a branched technology, applied in the implementation of digital logic, culturing cells alone, numbering, sending fluids, pumping fluids, and determining the best response strips, and indeed, the system is also part of the field of light, which can solve expensive, FACS utilization limitations, difficult to manufacture and operate and other issues

Active Publication Date: 2015-10-28
NANOCELLECT BIOMEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, flow cytometers, especially those with sorting capabilities, still have a large footprint, are expensive, and are technically difficult to manufacture and operate
[0009] In other words, the FACS system has not fundamentally changed since its inception, and thus, for all practical purposes, the utilization of FACS is limited to the common core facilities of well-funded institutions

Method used

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  • Systems, apparatus, and methods for sorting particles
  • Systems, apparatus, and methods for sorting particles
  • Systems, apparatus, and methods for sorting particles

Examples

Experimental program
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Effect test

example 1

[0374] Example 1: First-stage CTC enrichment using a hydrodynamic microfluidic device.

[0375] The hydrodynamic properties can be used to obtain 100-fold or higher enrichment of CTCs prior to microFACS sorting (see Example 3). In an effort to achieve >100-fold enrichment, several helical channel devices were designed. Several helical enrichment structures were designed for the first-stage enrichment of CTCs. The helical device structure takes advantage of the physical properties of cells under laminar flow conditions, where inertial lift and Dean forces allow focusing and separation of cells. The balance between inertial lift and Dean's force allows balancing position for any type of sample based on size. This protocol results in efficient separation of target large cells from a mixture of smaller cells. The general structure and concept are shown in figure 2 A-2C, which has approximate dimensions. In the first step, a mixture of cells of different sizes (eg, CTCs in bl...

example 2

[0382] Example 2: Hydrodynamically assisted CTC-binding antibody

[0383] The design was tested in which antibodies were added to cancer cells and the mixture was injected through a hybrid structure to achieve rapid and efficient cell labeling. Several commercially available antibodies were tested against EpCAM, an epithelial marker of CTCs. The results indicated that samples could potentially be labeled by the hybrid architecture disposable chip in 1-2 minutes (compared to 20-60 minutes). This approach is possible in the OncoSorter system due to the disposable nature of the chip used in the microfluidic detector system. Initial tests showed excellent labeling efficiency. In some embodiments, the microfluidic detector system is automated and adapted for use in a clinical setting where there are no scientists trained in cell handling and flow cytometry.

[0384] Fluid dynamics simulation software is used to design and analyze the hybrid structure to improve the binding effic...

example 3

[0388] Example 3: Benchtop Microfluidic Detector for CTC Isolation

[0389] Microfluidic detectors were developed to complete the enumeration and isolation of CTCs. In some cases, in-house designed microfluidic fluorescence-activated cell sorters (microfluidic detectors) were modified for CTC isolation and analysis. A design modification of the alpha prototype maximizes collection efficiency to ensure that nearly all CTCs are sorted. AutoCAD designs were generated to fabricate microfluidic detectors using PDMS in the Nano3 and at the Lo lab at UCSD ( Figure 10 A-B). The main channel was designed to be 200 microns wide and split into three 50 micron sample collection channels with a height of 80 microns. Chip holders are also designed to allow for Figure 10 C) chip replacement. The holder ensures precise alignment of the microfluidic channels with the optical axis by using alignment marks already implemented into the device, thereby eliminating any potential fluctuations...

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Abstract

Provided here are cell detection systems, fluidic devices, structures and techniques related to particle and cell sorting and detection in fluid, for example sorting specific subpopulations of cell types. A method for verification of sorting of particles includes receiving a first detection signal that is associated with optical characteristics of a particle in a first channel. A sorting channel of a plurality of second channels is determined based on the first detection signal, thereby determining the sorting of the particle into the sorting channel based on the optical characteristics of the particle. A sorting signal for sorting the particle from the first channel into the sorting channel is transmitted. A second detection signal is received that is associated with the presence of the particle in the sorting channel. The sorting of the particle from the first channel into the sorting channel is verified based on the second detection signal.

Description

[0001] Cross References to Related Applications [0002] This application claims U.S. Provisional Application No. 61 / 794015, filed March 15, 2013, entitled "Systems, Apparatus, and Methods for Particle Sorting," and entitled "Systems and Methods for Cellular Detection," filed October 15, 2012. Priority to U.S. Provisional Application No. 61 / 714091, the disclosure of which is incorporated herein by reference in its entirety. [0003] statement of government support [0004] One or more inventions disclosed herein were made with United States Government support awarded by: National Institutes of Health (NIH) Grant Nos. HHSN2612011 00096C, 9R44GM103677-02, HHSN261201200072C, 8R43GM103470-2. The US Government has certain rights in the one or more inventions described. Background technique [0005] Flow cytometry (FC) devices and systems can be used to characterize and analyze particles in fluids, such as cells, based on their optical responses when interrogated in a serial fashi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/00
CPCG01N33/57492B01L3/502761B01L2200/0652B01L2300/0636B01L2300/087B01L2300/088B01L2300/0883G01N15/1429G01N15/1434G01N15/1459G01N15/1463G01N15/1484G01N21/6486G01N33/582G01N2015/1006G01N2015/1075G01N2015/1081G01N2015/1486G01N2015/149G01N2201/06113G01N2333/70596
Inventor S·H·曹J·莫拉基斯Y·罗T-F·吴I·奎格利K·庄P·蓬卡W·阿莱尼克
Owner NANOCELLECT BIOMEDICAL
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