Daun negokilo polysaccharide and application thereof in preparation of immunoregulatory and antineoplastic drug and functional food

A functional food and immune regulation technology, applied in the field of medicine, can solve problems such as no related research, and achieve the effects of inhibiting tumor growth, strong anti-angiogenesis, and inhibiting weight loss

Active Publication Date: 2015-11-04
MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As an important component of it, the two compounds have been found to have anti-diabetic activity and blood pressure-lowering effect in rec...

Method used

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  • Daun negokilo polysaccharide and application thereof in preparation of immunoregulatory and antineoplastic drug and functional food
  • Daun negokilo polysaccharide and application thereof in preparation of immunoregulatory and antineoplastic drug and functional food
  • Daun negokilo polysaccharide and application thereof in preparation of immunoregulatory and antineoplastic drug and functional food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1, Extraction, preparation and analysis of Mingyuecao polysaccharide

[0023] The preparation method of Mingyuechao polysaccharide is as follows: take 50g of Mingyuechao stem and leaf powder, add 20 times the mass of deionized water, reflux extraction at 80-100°C for 2 hours, centrifuge, take the supernatant, and add 5 times the mass of deionized water for precipitation, Reflux extraction at 80-100°C for 2 hours, centrifuge, combine the filtrate, add a certain amount of 95% ethanol to the filtrate to make the final concentration of ethanol 75%, ethanol precipitation overnight, centrifuge to remove the supernatant, the precipitate is deproteinized and decolorized, and freeze-dried. The finally obtained biologically active polysaccharide is the moon grass polysaccharide of the present invention.

[0024] According to the HPLC molecular weight determination of the described Crescent Moon grass polysaccharide, the weight average molecular weight is 20-2000KDa, the t...

Embodiment 2

[0025] Example 2, the promoting effect of Mingyuecao polysaccharide on the phagocytosis of neutral red by RAW264.7 cells

[0026] Mouse mononuclear macrophage RAW264.7 cells were cultured in DMEM high glucose medium containing 10% fetal bovine serum. Cells in the logarithmic growth phase were taken at 1.0×10 5 The concentration of cells / mL was inoculated in a 96-well plate in 5% CO 2 , 37°C, cultured in a constant temperature incubator with saturated humidity until the cells adhered to the wall (about 6-8h), and then added 6 concentrations of polysaccharides containing different concentrations (1,5,10,25,50,100μg / ml) , the positive drug is lentinan, the final concentration is 2 μg / ml, set 4 parallel wells for each concentration, take it out after 12 hours of culture, absorb the original medium, wash it once with PBS at room temperature, add 100 μL 0.075% neutral red dye to each well solution, continue to incubate for 30min, absorb the neutral red, wash twice with PBS, lyse w...

Embodiment 3

[0032] Example 3, the effect of Mingyuecao polysaccharide on promoting the secretion of nitric oxide from RAW264.7 cells

[0033] Mouse mononuclear macrophage RAW264.7 cells were cultured in DMEM high glucose medium containing 10% fetal bovine serum. Cells in the logarithmic growth phase were inoculated in 96-well plates at a concentration of 1.0x105 cells / mL, cultured in a constant temperature incubator with 5% CO2, 37°C, and saturated humidity until the cells adhered to the wall (about 6-8 hours), and then added There are 6 concentrations of polysaccharides of different concentrations (1, 5, 10, 25, 50, 100 μg / ml), the positive drug is lentinan, the final concentration is 2 μg / ml, 4 parallel wells are set for each concentration, and after 24 hours of incubation Take it out, take 50 μL of the culture medium into another 96-well plate, and detect it according to the instruction of the nitric oxide detection kit (Beyontian Bioreagent Co., Ltd.), that is, the Grignard reagent me...

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Abstract

The invention provides daun negokilo polysaccharide and application thereof in preparation of immunoregulatory and antineoplastic drug and functional food. The daun negokilo polysaccharide can promote mononuclear macrophage strain to devour neutral red and release nitric oxide, can promote proliferation of cell B and cell T, can remarkably improve spleen and thymus indexes of immunosuppressed mice, transformation and proliferation of the cell T and the cell B and leucocyte level of the immunosuppressed mice, can obviously inhibit growing of tumor tissue and can protect organism, inhibit lowering of body weight and increase body weight. Polysaccharide serves as a main functional component, has multiple healthcare functions, is purely natural in source, thereby being free of side effect, and can be developed to be immunoregulatory, antineoplastic and anti-angiogenic drug and healthcare products, thereby having good market application prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a bright moon grass polysaccharide and its application in the preparation of drugs and functional foods for immune regulation and antitumor. Background technique [0002] In recent years, low immune function, including primary and secondary immune function deficiency, deficiency or damage, has been widely concerned by researchers at home and abroad, because they themselves and the various complications induced, especially infection, often Make the patient's condition worse, difficult to treat, and can cause serious consequences, even life-threatening. Therefore, finding safe drugs to improve the body's immune function will save more patients, and has a good application prospect in the health care industry. [0003] In addition, tumor is a difficult topic in the medical field in the 21st century, and it is the disease with the highest mortality rate in the world. Fin...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P37/02A61P35/00A23L1/09
Inventor 管华诗郝杰杰周晓琳敦云楼于广利夏萱
Owner MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
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